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Course Content: Equipment and Computer Resources:
In addition to powerful analytical instrumentation, one
computer-controlled bioreactor will be available for each team of (2) students.
Students will also be able to monitor their experiments remotely, from their
home. Furthermore, in the lab, each student will have dedicated access to a PC
loaded with Internet-based software, and with sofware for data analysis, word
processing and graphic manipulation software. Some of the pieces of equipment that will be used in the course are pictured below.
Wave bioreactor. In this run, hybridoma cells were grown for production of monoclonal
antibodies.
(Photo courtesy J-F Hamel) |
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An E. coli fermentation for the production of collagen. The bioreactor's working volume is 5 liters. The tubes attached to the steel headplate deliver acid, base, and nutrient feed to the culture. The controller to the left of the bioreactor vessel uses tunable P-I-D controllers to maintain agitation, pH, temperature, and dissolved oxygen (DO) according to the process design parameters.
(Photo courtesy J-F Hamel) |
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The mass spectrometer (bottom right) measures the composition of the bioreactor inlet and outlet gas, allowing for the calculation of Oxygen Uptake Rate (OUR), Carbon Dioxide Evolution Rate (CER), oxygen mass transfer coefficient, and Respiratory Quotient (RQ). The rotary valve (upper left) cycles through the sample lines, allowing for the analysis of multiple bioreactors.
(Photo courtesy J-F Hamel)
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