Ion regulation in ion-poor acidic water by the blackskirt tetra, a fish native to the Amazon River

Physiological Zoology, Volume 70, Issue 4, July - August 1997, Pages 428-435
Gonzalez, R J;
Dalton, V M; Patrick, M L

 

 

Summary of Points:

·        The ionoregulatory capabilities of the blackskirt tetra (Gymnocorymbus ternetzi) were examined, which is native to ion-poor acidic waters of the Amazon River.

·        Examination of Na+ uptake, which was only slightly sensitive to the uptake blocker amiloride, revealed several specializations for uptake in these waters. Kinetic analysis of Na+ uptake (at pH 6.5) revealed a high maximum rate of uptake and a low Michaelis-Menten constant, which allows the tetras to take up Na+ at high rates even at very low water levels.

·        At pH 4.5, a pH where they experience sizable ion disturbances, they displayed several mechanisms to restore balance. Kinetic analysis at pH 4.5 revealed that the maximum uptake rate rose 67% while the Michaelis-Menten constant remained unchanged.

·        Further tests showed that the upregulation of Na+ uptake occurred within 12 h in response to a doubling of Na+ efflux. Despite these specializations of the Na+ uptake mechanism, blackskirt tetras were not especially tolerant of low pH. Upon exposure to pH 4.0, they experienced a massive loss of Na+ due to a fourfold increase of Na+ efflux (relative to pH 5.0) and an 80% inhibition of uptake.

·        Measurement of Na+ efflux in waters with different Ca2+ levels and in the presence of LaCl, a strong Ca2+ competitor, correlated the stimulation of Na+ efflux at low pH with a low branchial affinity for Ca2+. These tests indicate that blackskirt tetras possess abilities to resist the disruptive effects of moderately low pH but cannot survive in waters with a pH of 4.0 or less because of leaching of Ca2+ from branchial tight junctions, which stimulates ion losses.