Fall 2003
TR 10-11:30 1-150
Prof. JoAnne Stubbe
Introduction to methods used to elucidate the mechanism of enzyme-catalyzed reactions. Application of steady-state and presteady-state kinetics, isotope effect measurements, site-directed mutagenesis, and mechanism-based inhibitors as tools to investigate the mechanisms of enzymes that have been well-characterized structurally.
