HEADER HYDROLASE (SERINE PROTEINASE) 26-OCT-81 3PTN 3PTN 3
COMPND TRYPSIN (TRIGONAL, 2.4 M AMMONIUM SULFATE) (E.C.3.4.21.4) 3PTN 4
SOURCE BOVINE (BOS TAURUS) PANCREAS 3PTN 5
AUTHOR J.WALTER,W.STEIGEMANN,T.P.SINGH,H.BARTUNIK,W.BODE,R.HUBER 3PTN 6
REVDAT 6 09-APR-85 3PTNE 1 JRNL 3PTNE 1
REVDAT 5 14-MAR-85 3PTND 3 SEQRES ATOM 3PTND 1
REVDAT 4 23-FEB-84 3PTNC 1 REMARK 3PTNC 1
REVDAT 3 30-SEP-83 3PTNB 1 REVDAT 3PTNB 1
REVDAT 2 15-APR-82 3PTNA 1 REMARK 3PTNB 2
REVDAT 1 04-MAR-82 3PTN 0 3PTNB 3
JRNL AUTH J.WALTER,W.STEIGEMANN,T.P.SINGH,H.BARTUNIK,W.BODE, 3PTN 7
JRNL AUTH 2 R.HUBER 3PTN 8
JRNL TITL ON THE DISORDERED ACTIVATION DOMAIN IN 3PTN 9
JRNL TITL 2 TRYPSINOGEN. CHEMICAL LABELLING AND 3PTNE 2
JRNL TITL 3 LOW-*TEMPERATURE CRYSTALLOGRAPHY 3PTNE 3
JRNL REF ACTA CRYSTALLOGR.,SECT.B V. 38 1462 1982 3PTNE 4
JRNL REFN ASTM ACBCAR DK ISSN 0567-7408 107 3PTNE 5
REMARK 1 3PTN 14
REMARK 1 REFERENCE 1 3PTNC 2
REMARK 1 AUTH M.MARQUART,J.WALTER,J.DEISENHOFER,W.BODE,R.HUBER 3PTNC 3
REMARK 1 TITL THE GEOMETRY OF THE REACTIVE SITE AND OF THE 3PTNC 4
REMARK 1 TITL 2 PEPTIDE GROUPS IN TRYPSIN, TRYPSINOGEN AND ITS 3PTNC 5
REMARK 1 TITL 3 COMPLEXES WITH INHIBITORS 3PTNC 6
REMARK 1 REF ACTA CRYSTALLOGR.,SECT.B V. 39 480 1983 3PTNC 7
REMARK 1 REFN ASTM ASBSDK DK ISSN 0108-7681 622 3PTNC 8
REMARK 1 REFERENCE 2 3PTNC 9
REMARK 1 AUTH H.FEHLHAMMER,W.BODE,R.HUBER 3PTN 16
REMARK 1 TITL CRYSTAL STRUCTURE OF BOVINE TRYPSINOGEN AT 1.8 3PTN 17
REMARK 1 TITL 2 ANGSTROMS RESOLUTION. /II$. CRYSTALLOGRAPHIC 3PTN 18
REMARK 1 TITL 3 REFINEMENT, REFINED CRYSTAL STRUCTURE AND 3PTN 19
REMARK 1 TITL 4 COMPARISON WITH BOVINE TRYPSIN 3PTN 20
REMARK 1 REF J.MOL.BIOL. V. 111 415 1977 3PTN 21
REMARK 1 REFN ASTM JMOBAK UK ISSN 0022-2836 070 3PTNA 1
REMARK 1 REFERENCE 3 3PTNC 10
REMARK 1 AUTH W.BODE,P.SCHWAGER,R.HUBER 3PTN 24
REMARK 1 TITL STRUCTURAL STUDIES ON THE PANCREATIC TRYPSIN 3PTN 25
REMARK 1 TITL 2 INHIBITOR-TRYPSIN COMPLEX AND ITS FREE COMPONENTS. 3PTN 26
REMARK 1 TITL 3 STRUCTURE AND FUNCTION RELATIONSHIPS IN SERINE 3PTN 27
REMARK 1 TITL 4 PROTEASE INHIBITION AND CATALYSIS 3PTN 28
REMARK 1 REF MIAMI WINTER SYMP. V. 11 43 1976 3PTN 29
REMARK 1 REFN ASTM MIWSAE US ISSN 0097-0808 926 3PTN 30
REMARK 1 REFERENCE 4 3PTNC 11
REMARK 1 AUTH W.BODE,P.SCHWAGER 3PTN 32
REMARK 1 TITL THE REFINED CRYSTAL STRUCTURE OF BOVINE 3PTN 33
REMARK 1 TITL 2 BETA-*TRYPSIN AT 1.8 ANGSTROMS RESOLUTION. 3PTN 34
REMARK 1 TITL 3 /II$. CRYSTALLOGRAPHIC REFINEMENT, CALCIUM BINDING 3PTN 35
REMARK 1 TITL 4 SITE, BENZAMIDINE BINDING SITE AND ACTIVE SITE AT 3PTN 36
REMARK 1 TITL 5 $P*H 7.0 3PTN 37
REMARK 1 REF J.MOL.BIOL. V. 98 693 1975 3PTN 38
REMARK 1 REFN ASTM JMOBAK UK ISSN 0022-2836 070 3PTN 39
REMARK 1 REFERENCE 5 3PTNC 12
REMARK 1 AUTH H.FEHLHAMMER,W.BODE 3PTN 41
REMARK 1 TITL THE REFINED CRYSTAL STRUCTURE OF BOVINE 3PTN 42
REMARK 1 TITL 2 BETA-*TRYPSIN AT 1.8 ANGSTROMS RESOLUTION. 3PTN 43
REMARK 1 TITL 3 I. CRYSTALLIZATION, DATA COLLECTION AND APPLICATION 3PTN 44REMARK 1 TITL 4 OF PATTERSON SEARCH TECHNIQUES 3PTN 45
REMARK 1 REF J.MOL.BIOL. V. 98 683 1975 3PTN 46
REMARK 1 REFN ASTM JMOBAK UK ISSN 0022-2836 070 3PTN 47
REMARK 1 REFERENCE 6 3PTNC 13
REMARK 1 AUTH W.BODE,P.SCHWAGER 3PTN 49
REMARK 1 TITL THE SINGLE CALCIUM-BINDING SITE OF CRYSTALLINE 3PTN 50
REMARK 1 TITL 2 BOVINE BETA-TRYPSIN 3PTN 51
REMARK 1 REF /FEBS$ LETT. V. 56 139 1975 3PTN 52
REMARK 1 REFN ASTM FEBLAL NE ISSN 0014-5793 165 3PTN 53
REMARK 1 REFERENCE 7 3PTNC 14
REMARK 1 EDIT M.O.DAYHOFF 3PTN 55
REMARK 1 REF ATLAS OF PROTEIN SEQUENCE V. 5 105 1972 3PTN 56
REMARK 1 REF 2 AND STRUCTURE (DATA SECTION) 3PTN 57
REMARK 1 PUBL NATIONAL BIOMEDICAL RESEARCH FOUNDATION, 3PTN 58
REMARK 1 PUBL 2 SILVER SPRING,MD. 3PTN 59
REMARK 1 REFN ISBN 0-912466-02-2 435 3PTN 60
REMARK 2 3PTN 61
REMARK 2 RESOLUTION. 1.7 ANGSTROMS. 3PTN 62
REMARK 3 3PTN 63
REMARK 3 REFINEMENT. DEISENHOFER*S VERSION OF THE JACK AND LEVITT 3PTN 64
REMARK 3 REFINEMENT PROCEDURE COMBINING CRYSTALLOGRAPHIC AND ENERGY 3PTN 65
REMARK 3 REFINEMENT. THE DIAGONAL-MATRIX LEAST-SQUARES METHOD WAS 3PTN 66
REMARK 3 ALSO USED TO REFINE ISOTROPIC TEMPERATURE FACTORS. 3PTN 67
REMARK 3 (A.JACK,M.LEVITT,ACTA CRYST.,A34,931-935,1978). 3PTN 68
REMARK 3 THE R-VALUE FOR REFLECTIONS WITHIN THE SHELL 1.7 TO 6.5 3PTN 69
REMARK 3 ANGSTROMS IS 0.198. 3PTN 70
REMARK 4 3PTN 71
REMARK 4 AN OCCUPANCY OF 0.0 INDICATES THAT NO SIGNIFICANT ELECTRON 3PTN 72
REMARK 4 DENSITY WAS FOUND IN THE FINAL FOURIER MAP. 3PTN 73
REMARK 5 3PTNA 2
REMARK 5 CORRECTION. INSERT MISSING CODEN FOR REFERENCE 1. 3PTNA 3
REMARK 5 15-APR-82. 3PTNA 4
REMARK 6 3PTNB 4
REMARK 6 CORRECTION. INSERT REVDAT RECORDS. 30-SEP-83. 3PTNB 5
REMARK 7 3PTNC 15
REMARK 7 CORRECTION. INSERT NEW PUBLICATION AS REFERENCE 1 AND 3PTNC 16
REMARK 7 RENUMBER THE OTHERS. 23-FEB-84. 3PTNC 17
REMARK 8 3PTND 2
REMARK 8 CORRECTION. CHANGE RESIDUE 165 FROM ASN TO ASP AND CHANGE 3PTND 3
REMARK 8 RESIDUE 186 FROM GLN TO GLU, UPON DEPOSITORS INSTRUCTIONS. 3PTND 4
REMARK 8 REVISE ATOM AND SEQRES RECORDS ACCORDINGLY. 14-MAR-85. 3PTND 5
REMARK 9 3PTNE 6
REMARK 9 CORRECTION. UPDATE JRNL REFERENCE TO REFLECT PUBLICATION. 3PTNE 7
REMARK 9 09-APR-85. 3PTNE 8
SEQRES 1 223 ILE VAL GLY GLY TYR THR CYS GLY ALA ASN THR VAL PRO 3PTN 74
SEQRES 2 223 TYR GLN VAL SER LEU ASN SER GLY TYR HIS PHE CYS GLY 3PTN 75
SEQRES 3 223 GLY SER LEU ILE ASN SER GLN TRP VAL VAL SER ALA ALA 3PTN 76
SEQRES 4 223 HIS CYS TYR LYS SER GLY ILE GLN VAL ARG LEU GLY GLU 3PTN 77
SEQRES 5 223 ASP ASN ILE ASN VAL VAL GLU GLY ASN GLU GLN PHE ILE 3PTN 78
SEQRES 6 223 SER ALA SER LYS SER ILE VAL HIS PRO SER TYR ASN SER 3PTN 79
SEQRES 7 223 ASN THR LEU ASN ASN ASP ILE MET LEU ILE LYS LEU LYS 3PTN 80
SEQRES 8 223 SER ALA ALA SER LEU ASN SER ARG VAL ALA SER ILE SER 3PTN 81
SEQRES 9 223 LEU PRO THR SER CYS ALA SER ALA GLY THR GLN CYS LEU 3PTN 82
SEQRES 10 223 ILE SER GLY TRP GLY ASN THR LYS SER SER GLY THR SER 3PTN 83
SEQRES 11 223 TYR PRO ASP VAL LEU LYS CYS LEU LYS ALA PRO ILE LEU 3PTN 84
SEQRES 12 223 SER ASP SER SER CYS LYS SER ALA TYR PRO GLY GLN ILE 3PTND 6
SEQRES 13 223 THR SER ASN MET PHE CYS ALA GLY TYR LEU GLU GLY GLY 3PTND 7
SEQRES 14 223 LYS ASP SER CYS GLN GLY ASP SER GLY GLY PRO VAL VAL 3PTN 87
SEQRES 15 223 CYS SER GLY LYS LEU GLN GLY ILE VAL SER TRP GLY SER 3PTN 88
SEQRES 16 223 GLY CYS ALA GLN LYS ASN LYS PRO GLY VAL TYR THR LYS 3PTN 89
SEQRES 17 223 VAL CYS ASN TYR VAL SER TRP ILE LYS GLN THR ILE ALA 3PTN 90
SEQRES 18 223 SER ASN 3PTN 91
FTNOTE 1 3PTN 92
FTNOTE 1 SEE REMARK 4. 3PTN 93
HET CA 480 1 CALCIUM (II) ION 3PTN 94
FORMUL 2 CA CA1 ++ 3PTN 95
FORMUL 3 HOH *82(H2 O1) 3PTN 96
HELIX 1 H1 SER 164 ILE 176 1 SNGL ALPHA TURN,REST IRREG. 3PTN 97
HELIX 2 H2 LYS 230 VAL 235 5 CONTIGUOUS WITH H3 3PTN 98
HELIX 3 H3 SER 236 ASN 245 1 CONTIGUOUS WITH H2 3PTN 99
SSBOND 1 CYS 22 CYS 157 3PTN 100
SSBOND 2 CYS 42 CYS 58 3PTN 101
SSBOND 3 CYS 128 CYS 232 3PTN 102
SSBOND 4 CYS 136 CYS 201 3PTN 103
SSBOND 5 CYS 168 CYS 182 3PTN 104
SSBOND 6 CYS 191 CYS 220 3PTN 105
CRYST1 55.110 55.110 109.380 90.00 90.00 120.00 P 31 2 1 6 3PTN 106
ORIGX1 1.000000 .577350 0.000000 0.00000 3PTN 107
ORIGX2 0.000000 1.154701 0.000000 0.00000 3PTN 108
ORIGX3 0.000000 0.000000 1.000000 0.00000 3PTN 109
SCALE1 .018146 .010476 0.000000 0.00000 3PTN 110
SCALE2 0.000000 .020953 0.000000 0.00000 3PTN 111
SCALE3 0.000000 0.000000 .009142 0.00000 3PTN 112
ATOM 1 N ILE 16 51.997 15.638 48.154 1.00 16.35 3PTN 113
ATOM 2 CA ILE 16 52.054 14.531 49.128 1.00 16.35 3PTN 114
ATOM 3 C ILE 16 53.326 14.579 49.971 1.00 16.35 3PTN 115
ATOM 4 O ILE 16 54.442 14.581 49.399 1.00 16.35 3PTN 116
ATOM 5 CB ILE 16 52.014 13.177 48.379 1.00 16.35 3PTN 117
ATOM 6 CG1 ILE 16 50.788 13.051 47.452 1.00 17.77 3PTN 118
ATOM 7 CG2 ILE 16 52.126 11.959 49.318 1.00 17.77 3PTN 119
ATOM 8 CD1 ILE 16 49.487 12.807 48.245 1.00 17.77 3PTN 120
ATOM 9 N VAL 17 53.168 14.567 51.274 1.00 13.81 3PTN 121
ATOM 10 CA VAL 17 54.289 14.479 52.218 1.00 13.81 3PTN 122
LOCUS BTTRYPAP 805 bp RNA MAM 06-JUN-1991
DEFINITION Bovine mRNA for pancreatic anionic trypsinogen.
ACCESSION X54703
KEYWORDS trypsin.
SOURCE cow.
ORGANISM Bos taurus
Eukaryotae; mitochondrial eukaryotes; Metazoa/Eumycota group;
Metazoa; Eumetazoa; Bilateria; Coelomata; Deuterostomia; Chordata;
Vertebrata; Gnathostomata; Osteichthyes; Sarcopterygii; Choanata;
Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Artiodactyla;
Ruminantia; Pecora; Bovoidea; Bovidae; Bovinae; Bos.
REFERENCE 1 (bases 1 to 805)
AUTHORS Le Huerou,I.
TITLE Direct Submission
JOURNAL Submitted (28-AUG-1990) to the EMBL/GenBank/DDBJ databases. Le
Huerou I., Centre National de la Recherche Scientifique, Centre de
Biochimie et de Biologie Moleculaire, CBM3, 31, Chemin Joseph
Aiguier, B.P. 71, 13402 Marseille Cedex 9, France
REFERENCE 2 (bases 1 to 805)
AUTHORS Le Huerou,I., Wicker,C., Guilloteau,P., Toullec,R. and
Puigserver,A.
TITLE Isolation and nucleotide sequence of cDNA clone for bovine
pancreatic anionic trypsinogen. Structural identity within the
trypsin family
JOURNAL Eur. J. Biochem. 193 (3), 767-773 (1990)
MEDLINE 91065383
COMMENT Data kindly reviewed (05-FEB-1991) by Huerou I.
NCBI gi: 829
FEATURES Location/Qualifiers
source 1..805
/organism="Bos taurus"
/strain="Friesian Holstein"
/dev_stage="adult"
/tissue_type="pancreas"
/clone_lib="pUC9"
/clone="PB10"
mRNA <1..805
CDS 9..752
/EC_number="3.4.21.4"
/note="NCBI gi: 830"
/codon_start=1
/product="trypsinogen anionic precursor"
/translation="MHPLLILAFVGAAVAFPSDDDDKIVGGYTCAENSVPYQVSLNAG
YHFCGGSLINDQWVVSAAHCYQYHIQVRLGEYNIDVLEGGEQFIDASKIIRHPKYSSW
TLDNDILLIKLSTPAVINARVSTLLLPSACASAGTECLISGWGNTLSSGVNYPDLLQC
LVAPLLSHADCEASYPGQITNNMICAGFLEGGKDSCQGDSGGPVACNGQLQGIVSWGY
GCAQKGKPGVYTKVCNYVDWIQETIAANS"
sig_peptide 9..53
misc_feature 54..77
/product="activation peptide"
mat_peptide 78..749
/EC_number="3.4.21.4"
/product="trypsin"