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Recombinant DNA Resources

The following links are meant as a "virtual appendix" to the 7.02 Recombinant DNA Methods (RDM) manual. Enjoy!
New England Biolabs (NEB) NEB is a Massachusetts-company that is the leader in the isolation and production of restriction enzymes. Their website has a number of excellent resources related to restriction and other DNA/RNA modifying enzymes. Every laboratory scientist I know has a copy of the NEB catalog--it's just that useful!
Restriction Enzymes Overview This page from New England Biolabs gives an overview of restriction enzymes, specifically their role in nature and the three types of REs (Type I, Type II, and Type III).
The BLAST Tutorial Tutorial on searching online databases.
BLAST Tutorial Worksheet (pdf) Download this worksheet before beginning the BLAST tutorial!
BLAST tutorial answer key (pdf) Answers to the BLAST tutorial worksheet.
DNAi "DNA interactive" site from Cold Spring Harbor DNA Learning Center.
DNAi-Manipulations
(requires Macromedia Flash Player 6 or later)
Subpage of DNAi containing animations of RDM topics (restriction enzymes, ligations, PCR, etc.)

DNA in 3D!

This page from the University of Arizona Department of Chemistry contains some great 3D views of the DNA double helix, as well as G-C basepairing.
Chalfie paper on GFP The original Science paper describing GFP's use as a reporter of protein expression.
(accessed through MIT Libraries; for MIT users only)