MICROBIAL GENETICS DAY THREE

Today you will make P1 transducing lysates from each of your Ara- mutants. You will also have a chance to look at the results of your patching from Day 2.

MAKING P1 TRANSDUCING LYSATES

In prepartion for this part of the laboratory, you may want to review "transferring liquids via sterile technique" and "plating bacterial cultures" from earlier in the module.

RESULTS OF DAY TWO PATCHING

When you get your plates back, it may be useful to put the patching grids back on to help you orient your plate.	To analyze your data, line up the plates from minimal to rich (that is, in the same order that you patched them). Record the data for EACH grid square, and determine the phenotype of your mutants.

Here are closer views of a patched M9 plate, Mac Ara Kan plate, and LB Xgal Kan plates used for phenotypic characterization.

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