Virtual Lab

The goal of the "Virtual Lab" is to provide you with pictures of many of the reagents/apparatus that you will be using in the 7.02 laboratory. We hope this will help you visualize the procedures that you are reading about in the laboratory manual--and thus allow you to be better prepared for lab!

Take a tour of the 7.02 teaching lab!

Protein Biochemistry

Lab Day	What's in the "Virtual Lab"?
PBC 1 Virtual Lab	cell lysis, centrifugation, Bgal assay pictures
PBC 2 Virtual Lab	PD-10 and DEAE column pictures
PBC 4 Virtual Lab, Part One *	Setting up SDS-PAGE apparatus, loading/running gel pictures
PBC 4 Virtual Lab, Part Two	Bradford assay, Coomassie staining/destaining, gel drying pictures
PBC 5 Virtual Lab*	Setting up the Western "sandwich," elecroblotter, blocking membrane pictures
*there are no pictures for PBC Days 3 and 6

Microbial Genetics

Lab Day	What's in the "Virtual Lab"?
GEN 1 Virtual Lab, Part One	Transferring liquid from one container to another using sterile technique
GEN 1 Virtual Lab, Part Two	Transposon mutagenesis, plating bacterial cultures, and streaking bacterial strains on agar plates
GEN 2 Virtual Lab	Sample results of Day 1 streaking and pictures related to phenotypic characterization ("patching")
GEN 3 Virtual Lab	Pictures of patched plates from Day 2
GEN 4-6 Virtual Lab	Pictures related to P1 transduction

Recombinant DNA Methods

Lab Day	What's in the "Virtual Lab"?
RDM 1 Virtual Lab	Agarose gel electrophoresis, isolating DNA fragments from a low-melt gel
RDM 2 and 2.5 Virtual Lab	Pictures related to bacterial transformation and PCR
RDM 3-5 Virtual Lab	No new pictures, but links to related "virtual lab" pages!