|
LB Agar 1% |
Amount |
Published |
Notes |
|
Typtone |
10 gm |
1% |
|
|
Yeast Extract |
5 gm |
0.5% |
|
|
NaCl |
5 gm |
0.5% |
For phage growth |
|
NaOH 1 N |
1ml |
1mM |
|
|
Agar |
10 gm |
1% |
|
|
Water |
1 liter |
LB Broth Amount Conc. Notes Tryptone 10 gm 1% Yeast Extract 5 gm 0.5% NaCl 5 gm 0.5% For phage growth NaOH 1 N 1ml 1mM Water 1 liter
Super Broth Amount Conc. Notes Tryptone 32 gm 3.2% Yeast Extract 20 gm 2% NaCl 5 gm 0.5% For phage growth NaOH 1 N 7 ml 7 mM Water 1 liter
Soft Agar Amount Conc. Notes Nutrient Broth 8 gms 0.8% NaCl 5gms 0.5% Agar 6.5gm 0.65 % Water 1 Liter
Dilution Fluid Amount Conc. Notes Tryptone 1 gm 0.1% NaCl 7 gm 0.7% MgS04 0.5 gm 2mM Water