Streaking Phage

CBB - last updated: September 1, 1998

(1) make lawn of 7155 using:

- two drops of plating bacteria

- added to 2.5 ml soft agar in small culture tube in warmer

- vortex quickly

- spread on plate surface

- allow ~10 minutes to set

(2) sterilize platinum wire in flame.

(3) use a 1 ml glass pipet to touch surface of phage stock. then touch center of a lawn transferring phage stock moisture to the plate.

(4) use platinum wire to drag through the droplet in multiple directions.

(5) place in 30degC room overnight.