CHP
|
71/2% 10% |
|||||||
|
SEPARATING |
1X |
2X |
3X |
4X |
1x |
2x |
3x |
|
Acrylamide 30:08 |
5 |
10 |
15 |
20 |
6.6 |
13.2 |
19.8 |
|
Water (ml) |
10 |
20 |
30 |
40 |
8.4 |
16.8 |
25.2 |
|
Lower Buffer |
5 |
10 |
15 |
20 |
5 |
10 |
15 |
|
10% APS (ul) |
100 |
200 |
300 |
400 |
100 |
200 |
300 |
|
TEMED (ul) |
10 |
20 |
30 |
40 |
10 |
20 |
30 |
|
12% 14% 16% |
||||
|
SEPARATING |
1X |
1X |
2X |
1.5x |
|
Acrylamide 30:08 |
8.4 |
9.3 |
18.6 |
16 |
|
Water (ml) |
6.6 |
5.7 |
11.4 |
6.5 |
|
Lower Buffer |
5 |
5 |
10 |
7.5 |
|
10% APS (ul) |
100 |
100 |
200 |
150 |
|
TEMED (ul) |
10 |
10 |
20 |
15 |
|
STACKING |
1X |
2X |
3X |
4X |
|
Acrylamide 30:08 |
1 |
2 |
3 |
4 |
|
Water (ml) |
6.5 |
13 |
19.5 |
26 |
|
Upper Buffer |
2.5 |
5 |
7.5 |
10 |
|
10% APS (ul) |
50 |
100 |
150 |
200 |
|
TEMED (ul) |
10 |
20 |
30 |
40 |
BioRad Acrylamide 30% T-> 150gm acrylamide/bis + 362 ml ddH20