Solutions for high pH native gels

7x Native Gel Upper (Stacking) Buffer

5.7 g Tris base

pH to 6.7 with H3PO4

water to 100 mL

4x Native Gel Lower (Separating) Buffer

18.2 g Tris base

pH to 8.9 with HCl

water to 100 mL

50x Running Buffer

7.5 g Tris base

36 g Glycine

Water to 250 mL

3x Sample Buffer

3 mL glycerol

0.6 mL 50x running buffer

6.4 mL H2O

bromophenol blue

Sample Gel Recipes:

Separating (Lower): 7.5%

H2O 6 mL

lower buffer 3 mL

30/0.8% acryl/bis 3 mL

TEMED 7 µl

10% APS 84 µl

Stacking (Upper) 4.3%

H2O 5 mL

upper buffer 1 mL

30/0.8% acryl/bis 1 mL

TEMED 8 µl

10% APS 40 µl