Roger D. Kamm, Ph.D.
Cecil and Ida Green Distinguished Professor of Biological and Mechanical Engineering
Lab web site
Phone: (617) 253-5330
Fax: (617) 258-8559
Administrative Assistant: Sossy Megerdichian
Courses: Fluid Mechanics (2.25; 2.005), Molecular, Cellular and Tissue Biomechanics (20.310, 20.410) and Quantitative Physiology: Organ Transport Systems (6.022J)
Current research activities in the Kamm Laboratory can be grouped into three broad categories:
Tissue engineering and microfluidics. Interest in developing vascular networks in vitro has led to new activities in the design and fabrication of novel microfluidic systems that provide an environment for growing three-dimensional vascular networks within a microfluidic platform that allows for simultaneous control over a wide range of biochemical factors and biophysical factors. Time-lapse imaging also provides the opportunity for real-time control of these factors to achieve a desired outcome. Computational models are also being developed to simulate the process of angiogenesis, and to couple with the experiments.
Our laboratory also develops new scaffolds for tissue engineering comprised of self-assembling peptides. These scaffold have the advantage of having a filamentous structure similar to that of the extracellular matrix in terms of stiffness and fibril size and density. In addition, the peptides can be functionalized to present specific growth factors or cytokines required for specific tissue function. Current efforts are directed toward the development of microvascular networks, the major obstacle in the creation of vascularized organs.
Cellular rheology. The cytoskeleton is comprised of a filamentous network (actin, microtubules and intermediate filaments) capable of polymerization, depolymerization, cross-linking, and branching in response to both biochemical and mechanical stimuli. Our laboratory is conducting experiments in living cells to probe this dynamic behavior and also developing computational models to simulate it.
Multi-scale modeling is one of our goals in which we plan to couple cytoskeletal modeling with molecular dynamics simulation of cytoskeletal proteins, or proteins that link the cytoskeleton to the extracellular matrix (focal adhesions) or neighboring cells (adherens junctions).
Molecular mechanics. Current projects include studies into the formation and structure of oligomers and protofibrils of amyloid-b protein using computational (molecular dynamics) and experimental approaches. These oligomers are of interest because of their central role in causing the cytotoxicity associated with Alzheimer’s disease. Other studies are directed toward understanding the conformational changes that occur in certain intracellular proteins, and the changes in protein binding affinities and enzymatic activity that result from the changes in conformation. This process, known as mechanotransduction, is the fundamental mechanism by which cells sense mechanical force.
Click here for a complete list of publications
Kothapalli CR, van Veen E, de Valence S, Chung S, Zervantonakis IK, Gertler FB, Kamm RD. A high-throughput microfluidic assay to study neurite response to growth factor gradients. Lab Chip. 2011 Feb 7; 11 (3) :497-507. PMID:21107471.
Zervantonakis IK, Kothapalli CR, Chung S, Sudo R, Kamm RD. Microfluidic devices for studying heterotypic cell-cell interactions and tissue specimen cultures under controlled microenvironments. Biomicrofluidics. 2011 Mar 30; 5(1):13406. PMID:21522496.
Jeon JS, Chung S, Kamm RD, Charest JL. Hot embossing for fabrication of a microfluidic 3D cell culture platform. Biomed Microdevices. 2011 Apr; 13(2):325-33. PMID:21113663; PMC3117225.
Wood L, Kamm R, Asada H. Stochastic modeling and identification of emergent behaviors of an endothelial cell population in angiogenic pattern formation. 2011 May; 30(6)659-677.
Wan CR, Chung S, Kamm RD. Differentiation of embryonic stem cells into cardiomyocytes in a compliant microfluidic system. Ann Biomed Eng. 2011 Jun; 39 (6) :1840-7. PMID:21336802.
Polacheck WJ, Charest JL, Kamm RD. Interstitial flow influences direction of tumor cell migration through competing mechanisms. Proc Natl Acad Sci U S A. 2011 Jul 5; 108 (27):11115-20. PMID:21690404; PMCID: PMC3131352.
Shin Y, Jeon JS, Han S, Jung GS, Shin S, Lee SH, Sudo R, Kamm RD, Chung S. In vitro 3D collective sprouting angiogenesis under orchestrated ANG-1 and VEGF gradients. Lab Chip. 2011 Jul 7; 11 (13) :2175-81. PMID:21617793.
Kim T, Hwang W, Kamm RD. Dynamic role of cross-linking proteins in actin rheology. Biophys J. 2011 Oct 5;101(7):1597-603. PMID: 21961585
Borau C, Kamm RD, García-Aznar JM. Mechano-sensing and cell migration: a 3D model approach. Phys Biol. 2011 Dec;8(6):066008. Epub 2011 Nov 25. PMID: 22120116
Murrell M, Kamm R, Matsudaira P. Substrate viscosity enhances correlation in epithelial sheet movement. Biophys J. 2011 Jul 20;101(2):297-306.
Murrell M, Kamm R, Matsudaira P. Tension, free space, and cell damage in a microfluidic wound healing assay. PLoS One. 2011;6(9):e24283. Epub 2011 Sep 6.
Luo Y, Zervantonakis IK, Oh SB, Kamm RD, Barbasthathis G. Spectrally resolved multidepth fluorescence imaging. J Biomed Opt. 2011 Sep;16(9): 096015. PMID: 21950929.
Jeong GS, Han S, Shin Y, Kwon GH, Kamm RD, Lee SH, Chung S. Sprouting angiogenesis under a chemical gradient regulated by interaction with endothelial monolayer in microfluidic platform, Anal Chem. Epub 2011 Oct 10. PMID: 21985643.
Rahim NA, Pelet S, Kamm RD, So PT. Methodological considerations for global analysis of cellular FLIM/FRET measurements. J Biomed Opt. 2012 Feb;17(2):026013.
Wong KH, Chan JM, Kamm RD, Tien J. Microfluidic Models of Vascular Functions. Annu Rev Biomed Eng. 2012 Apr 23. [Epub ahead of print]
Farahat WA, Wood LB, Zervantonakis IK, Schor A, Ong S, Neal D, Kamm RD, Asada H. Ensemble Analysis of Angiogenic Growth in Three-Dimensional Microfluidic Cell Cultures, PLoS One, 7(5), 2012.
Yuchun Liu, Swee-Hin Teoh, Mark SK Chong, Eddy SM Lee, Citra N Mattar, Nau’shil K Randhawa, Zhiyong Zhang, Roger D Kamm, Nicholas M Fisk, Mahesh Choolani, Jerry K Y Chan. Vasculogenic and Osteogenesis-Enhancing Potential of Human Umbilical Cord Blood Endothelial Colony-Forming Cells. Stem Cells (2012) IN PRESS.
Choong Kim, Seok Chung, Liu Yuchun, Min-Cheol Kim Jerry K. Y. Chan, H. Harry Asada and Roger D. Kamm In vitro angiogenesis assay for the study of cell encapsulation therapy. Lab Chip, 2012, DOI:10.1039/C2LC40182G