Jacquin C. Niles, M.D., Ph.D.

Jacquin C. Niles, M.D., Ph.D.
Assistant Professor, Department of Biological Engineering

Email: jcniles@mit.edu
Office: 16-733
Phone: (617) 324-3701

Administrative Assistant: Denise MacPhail

Research Focus

Research in my lab emphasizes the development of new molecular tools aimed at facilitating investigation of human pathogens that have been difficult to study due to an inability to readily and efficiently manipulate their genomes.  The lab is especially focused on Plasmodium falciparum, the parasitic agent causing malaria, of which there are some 600 million cases and 1-2 million deaths annually in developing countries. While knowledge of this complex pathogen is rapidly improving in the post-genomic era, many fundamental aspects of its biology and success as a human pathogen remain inaccessible.

A major effort in the lab to overcome this difficulty uses fundamental biological and chemical concepts to design and build chemically regulated modules that can be used to control gene expression at both the transcriptional and post-transcriptional level.  Systematically, the biochemical and biophysical parameters governing the behavior of these synthetic modules will be established, and the insights gained will be used to optimize their design and function.  In addition to applying these tools to investigate P. falciparum biology, we are interested in integrating them with existing technologies to improve the range of useful programmed cellular behaviors achievable in other biological systems while using the smallest set of easily controlled inputs.

Publications

  1. Niles JC, Marletta MA.
    Related Articles, Compound (MeSH Keyword), Gene, Protein (RefSeq), Protein Clusters, Substance (MeSH Keyword), Taxonomy via GenBank, GEO Profiles, LinkOutUtilizing RNA aptamers to probe a physiologically important heme-regulated cellular network.
    ACS Chem Biol. 2006 Sep 19;1(8):515-24. Erratum in: ACS Chem Biol. 2006 Oct 20;1(9):601.
    PMID: 17168539 [PubMed - indexed for MEDLINE]

  2. Niles JC, Wishnok JS, Tannenbaum SR.
    Related Articles, Compound (MeSH Keyword), Substance (MeSH Keyword), LinkOutPeroxynitrite-induced oxidation and nitration products of guanine and 8-oxoguanine: structures and mechanisms of product formation.
    Nitric Oxide. 2006 Mar;14(2):109-21. Epub 2005 Dec 13. Review.
    PMID: 16352449 [PubMed - indexed for MEDLINE]

  3. Henderson PT, Neeley WL, Delaney JC, Gu F, Niles JC, Hah SS, Tannenbaum SR, Essigmann JM.
    Related Articles, Compound (MeSH Keyword), Substance (MeSH Keyword), Cited in PMC, LinkOutUrea lesion formation in DNA as a consequence of 7,8-dihydro-8-oxoguanine oxidation and hydrolysis provides a potent source of point mutations.
    Chem Res Toxicol. 2005 Jan;18(1):12-8.
    PMID: 15651843 [PubMed - indexed for MEDLINE]

  4. Niles JC, Wishnok JS, Tannenbaum SR.
    Related Articles, Compound (MeSH Keyword), Substance (MeSH Keyword), LinkOutSpiroiminodihydantoin and guanidinohydantoin are the dominant products of 8-oxoguanosine oxidation at low fluxes of peroxynitrite: mechanistic studies with 18O.
    Chem Res Toxicol. 2004 Nov;17(11):1510-9.
    PMID: 15540949 [PubMed - indexed for MEDLINE]

  5. Niles JC, Wishnok JS, Tannenbaum SR.
    Related Articles, Compound (MeSH Keyword), Substance (MeSH Keyword), LinkOutMass spectrometric identification of 4-hydroxy-2,5-dioxo-imidazolidine-4-carboxylic acid during oxidation of 8-oxoguanosine by peroxynitrite and KHSO5/CoCl2.
    Chem Res Toxicol. 2004 Nov;17(11):1501-9.
    PMID: 15540948 [PubMed - indexed for MEDLINE]

  6. Yu H, Niles JC, Wishnok JS, Tannenbaum SR.
    Related Articles, Compound (MeSH Keyword), Substance (MeSH Keyword), LinkOutSpirodihydantoin is a minor product of 5-hydroxyisourate in urate oxidation.
    Org Lett. 2004 Sep 16;6(19):3417-20.
    PMID: 15355066 [PubMed - indexed for MEDLINE]

  7. Lee JM, Niles JC, Wishnok JS, Tannenbaum SR.
    Related Articles, Compound (MeSH Keyword), Substance (MeSH Keyword), Cited in PMC, LinkOutPeroxynitrite reacts with 8-nitropurines to yield 8-oxopurines.
    Chem Res Toxicol. 2002 Jan;15(1):7-14.
    PMID: 11800591 [PubMed - indexed for MEDLINE]

  8. Niles JC, Wishnok JS, Tannenbaum SR.
    Related Articles, Compound (MeSH Keyword), Substance (MeSH Keyword), Cited in PMC, LinkOutAnovel nitroimidazole compound formed during the reaction of peroxynitrite with 2',3',5'-tri-O-acetyl-guanosine.
    J Am Chem Soc. 2001 Dec 12;123(49):12147-51.
    PMID: 11734012 [PubMed - indexed for MEDLINE]

  9. Niles JC, Wishnok JS, Tannenbaum SR.
    Related Articles, Compound (MeSH Keyword), Substance (MeSH Keyword), Cited in PMC, LinkOutSpiroiminodihydantoin is the major product of the 8-oxo-7,8-dihydroguanosine reaction with peroxynitrite in the presence of thiols and guanosine photooxidation by methylene blue.
    Org Lett. 2001 Apr 5;3(7):963-6.
    PMID: 11277770 [PubMed - indexed for MEDLINE]

  10. Niles JC, Wishnok JS, Tannenbaum SR.
    Related Articles, Compound (MeSH Keyword), Substance (MeSH Keyword), Cited in PMC, LinkOutAnovel nitration product formed during the reaction of peroxynitrite with 2',3',5'-tri-O-acetyl-7,8-dihydro-8-oxoguanosine: N-nitro-N'-[1-(2,3,5-tri-O-acetyl-beta-D-erythro-pentofuranosyl)- 2, 4-dioxoimidazolidin-5-ylidene]guanidine.
    Chem Res Toxicol. 2000 May;13(5):390-6.
    PMID: 10813656 [PubMed - indexed for MEDLINE]

  11. Niles JC, Burney S, Singh SP, Wishnok JS, Tannenbaum SR.
    Related Articles, Cited Articles, Free in PMC, Cited in PMC, LinkOutPeroxynitrite reaction products of 3',5'-di-O-acetyl-8-oxo-7, 8-dihydro-2'-deoxyguanosine.
    Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11729-34.
    PMID: 10518518 [PubMed - in process]

  12. Burney S, Niles JC, Dedon PC, Tannenbaum SR.
    Related Articles, Compound (MeSH Keyword), Substance (MeSH Keyword), Cited in PMC, LinkOutDNA damage in deoxynucleosides and oligonucleotides treated with peroxynitrite.
    Chem Res Toxicol. 1999 Jun;12(6):513-20.
    PMID: 10368314 [PubMed - indexed for MEDLINE]

  13. Tretyakova NY, Niles JC, Burney S, Wishnok JS, Tannenbaum SR.
    Related Articles, Compound (MeSH Keyword), Substance (MeSH Keyword), Cited in PMC, LinkOutPeroxynitrite-induced reactions of synthetic oligonucleotides containing 8-oxoguanine.
    Chem Res Toxicol. 1999 May;12(5):459-66.
    PMID: 10328757 [PubMed - indexed for MEDLINE]

  14. Burney S, Caulfield JL, Niles JC, Wishnok JS, Tannenbaum SR.
    Related Articles, Compound (MeSH Keyword), Substance (MeSH Keyword), Cited in PMC, LinkOutThe chemistry of DNA damage from nitric oxide and peroxynitrite.
    Mutat Res. 1999 Mar 8;424(1-2):37-49. Review.
    PMID: 10064848 [PubMed - indexed for MEDLINE]

  15. Butt JN, Niles J, Armstrong FA, Breton J, Thomson AJ.
    Related Articles, Compound (MeSH Keyword), Substance (MeSH Keyword), Cited in PMC, LinkOutFormation and properties of a stable 'high-potential' copper-iron-sulphur cluster in a ferredoxin.
    Nat Struct Biol. 1994 Jul;1(7):427-33.
    PMID: 7664060 [PubMed - indexed for MEDLINE]

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