Supplemental Table 1.  A subset of genes regulated by 20% glucose.  The data shown were used to generate Fig. 6A.  Fold change is shown for cells grown in YPD+20% glucose for 30/60/120/240 minutes.   The complete set of microarray data can be downloaded from our web site (see Methods).

 

A. A subset of genes up-regulated by 20% glucose.

ID

Name

Gene Function

Fold Change

YFL014W

HSP12

Heat shock protein of 12kDa, induced by heat, osmostress, oxidative stress and stationary phase

31/25.2/6.4/8.1

YDL222C

 

Protein with similarity to Sur7p

16.2/2.3/1.5/1.8

YHR087W

 

Protein of unknown function

15.9/5.5/4.7/3.9

YHR033W

 

Protein with similarity to Pro1p

15.2/2.9/1.3/1.3

YGR088W

CTT1

Catalase T (cytosolic)

15.2/2.5/1.1/1.4

YOL084W

 

Protein of unknown function; member of the major facilitator superfamily (MFS)

14.6/2.6/1.3/1.2

YDL223C

 

Protein of unknown function

12.9/2.5/0.9/1

YOL150C

 

Protein of unknown function

12.9/3.1/3.3/3.4

YGR144W

THI4

Thiamine-repressed protein essential for growth in the absence of thiamine

12.5/3.4/2.6/2.8

YDL023C

 

Protein of unknown function

11.9/3.1/3.2/2.9

YPL223C

GRE1

Protein induced by osmotic stress

11.9/2.4/1.1/1.2

YGR049W

SCM4

Protein that suppresses temperature-sensitive allele of CDC4 when overexpressed

10/2.3/2.6/2.7

YMR169C

ALD3

Probable aldehyde dehydrogenase (NAD(P)+), likely cytosolic

9.3/2.6/1.3/1.5

YOL151W

GRE2

Protein involved in diamide tolerance and induced by osmotic stress

9.3/3.3/2.7/2.4

YMR170C

ALD2

Aldehyde dehydrogenase (NAD(P)+), likely cytosolic

8.3/2/1.3/1.6

YDL022W

GPD1

Glycerol-3-phosphate dehydrogenase (NAD+) (cytoplasmic); involved in glycerol production converting glycerol-3-phosphate and NAD+ to dihydroxyacetone phosphate and NADH

8/2.1/2/1.7

YML131W

 

Protein of unknown function

7.8/3.2/2.2/1.2

YJL088W

ARG3

Ornithine carbamyltransferase, catalyzes the sixth step in the arginine biosynthesis pathway

7.7/1.8/1.8/1.8

YJL108C

 

Protein of unknown function; member of the major facilitator superfamily (MFS)

7/5.4/1.9/1.8

YJL116C

NCA3

Protein involved in regulation of synthesis of Atp6p and Atp8p

6.8/4.7/3.6/2.9

YMR173W

DDR48

Stress protein induced by heat shock, DNA damage, or osmotic stress

5.9/3.7/1.3/1.4

YJL107C

 

Protein of unknown function

5.8/3.4/1.9/1.8

YHR103W

SBE22

Protein required for bud growth

5.6/1.8/1.6/1.7

YOR177C

 

Protein of unknown function, putative coiled-coil protein induced early in sporulation

5.5/3.3/1.1/1.2

YMR090W

 

Protein with similarity to malate dehydrogenases

5.3/3.5/1.8/2.1

YMR250W

 

Protein with similarity to glutamate decarboxylase

5.1/1.7/1/1.1

YGR043C

 

Protein with strong similarity to Tal1p

4.8/3.2/1.7/1.5

YOR161C

 

Protein of unknown function, member of the major facilitator superfamily (MFS)

4.8/3.9/1.7/1.6

YHR095W

 

Protein of unknown function

4.7/2.5/2/1.6

YHR039C

 

Protein with strong similarity to aldehyde dehydrogenases

4.6/2.1/1.6/2.1

YER062C

HOR2

DL-glycerol phosphate phosphatase

4.5/2.3/1.9/1.7

YML070W

DAK1

Putative dihydroxyacetone kinase

4/2.4/1.5/1.5

YHR139C

SPS100

Sporulation specific protein involved in spore wall formation; first appears at 12h of sporulation

3.6/1.7/1.5/1.3

YLR108C

 

Protein of unknown function

3.5/2.7/2.9/2.6

YGR059W

SPR3

Sporulation-specific septin

3.4/2/1.2/1.2

YMR173W-A

 

Stress protein induced by heat shock, DNA damage, or osmotic stress

3.4/2.5/1.4/1.2

YOL053C-A

SPE2

Stress protein induced by DNA damage, heat shock, osmotic shock and oxidative stress

3.3/2.5/1.4/1.4

YBR222C

FAT2

Peroxisomal AMP-binding protein

3.2/1.6/1.3/1.2

YNR076W

PAU6

Protein with strong similarity to members of the PAU1 family

3/1.6/1.5/4.1

YLR338W

 

Protein of unknown function

2.9/1.5/1.2/1.6

YER119C-A

 

Protein of unknown function

2.9/1.6/0.9/0.9

YIL108W

 

Protein of unknown function

2.8/1.5/1.3/1.3

YFR022W

 

Protein with similarity to Rod1p

2.8/2.2/1.2/1.2

YJR078W

 

Protein with similarity to indoleamine 2,3-dioxygenase

2.5/1.6/1.1/1

YDR368W

YPR1

Protein with similarity to members of the aldo/keto reductase family

2.4/1.6/1.5/1.4

YGL141W

 

Putative ubiquitin-protein ligase

2.3/1.7/1.1/1.9

YJL093C

TOK1

Two-domain outwardly-rectifying voltage-gated potassium channel

2.3/1.6/1.2/1.1

YLR046C

 

Protein with similarity to Rtm1p

2.2/1.6/1.6/1.6

YLR030W

 

Protein of unknown function

2.2/1.6/1.3/1.2

YJR162C

 

Protein with similarity to subtelomerically-encoded proteins including Gin11p and Ykl225p

2.1/1.6/1.5/1.1

 


 

 

B. A subset of genes down-regulated by 20% glucose.

ID

Name

Gene Function

Fold Change

YJL218W

 

Protein with similarity to E. coli galactoside O-acetyltransferase

-5.5/-2.1/-1.2/-1.8

YJL217W

 

Protein of unknown function

-5.4/-2.2/-1/-1.4

YBL028C

 

Protein of unknown function

-3.4/-1.8/-1.2/-1.2

YLR214W

FRE1

Ferric and cupric reductase; acts on ferric iron chelates external to the cell

-3.3/-1.6/-1/-1

YOR342C

 

Protein of unknown function

-3.2/-1.7/-1.2/-1.3

YFR001W

 

Protein of unknown function

-2.9/-1.8/-1.2/-1.3

YNL078W

 

Protein of unknown function

-2.8/-1.6/1/-1.4

YDR357C

 

Protein of unknown function

-2.6/-1.9/-1/-1.2

YAL033W

POP5

Subunit of both RNase P and RNase MRP

-2.6/-1.6/-1.3/-1.2

YPR124W

CTR1

Copper transport protein; required for high-affinity uptake of copper

-2.6/-2.1/-1.5/-1.3

YIL127C

 

Protein of unknown function

-2.5/-1.6/-1.3/-1.2

YFR015C

GSY1

UDP-glucose-starch glucosyltransferase (glycogen synthetase) isoform 1

-2.5/-1.8/-3.1/-3.2

YGR177C

ATF2

Sterol O-acetyltransferase; acetylates certain toxic steroids such as pregnenolone to render them excretable

-2.5/-1.8/-1.4/-1.3

YGR035C

 

Protein of unknown function

-2.5/-1.8/-1.6/-1.9

YBR154C

RPB5

Shared subunit of RNA polymerases I, II, and III (ABC27)

-2.4/-1.6/-1/-1.1

YJR067C

YAE1

Essential protein of unknown function

-2.4/-1.5/-1.5/-1.3

YBR083W

TEC1

Transcriptional activator; involved with STE12 in pseudohyphal formation

-2.4/-1.7/-1.3/-1.3

YHR055C

CUP1-2

Metallothionein (copper chelatin); identical to Cup1Ap

-2.3/-1.9/-1.4/-2.4

YNL174W

 

Protein of unknown function

-2.3/-1.7/-1.3/-1.2

YOR294W

RRS1

Regulator of ribosome synthesis

-2.3/-1.7/-1.3/-1.1

YGL029W

CGR1

Predicted coiled-coil protein, down-regulated after diauxic shift

-2.3/-1.5/-1.4/-1.3

YPR082C

DIB1

Protein required for mitotic spindle formation or function

-2.3/-1.8/-1.2/-1.2

YLR460C

 

Protein with strong similarity to Ycr102p

-2.3/-1.8/-1.7/-1.7

YDR152W

 

Protein of unknown function

-2.3/-1.8/-1.4/-1.4

YOR004W

 

Protein of unknown function

-2.3/-1.6/-1.1/-1.1

YMR136W

GAT2

Protein of unknown function

-2.3/-1.7/-2.2/-2.2

YDL037C

 

Protein with similarity to glucan 1,4-alpha-glucosidase

-2.2/-1.8/-1.5/-1.5

YMR193C-A

 

Protein of unknown function

-2.2/-1.7/1.1/-1.1

YDR469W

 

Protein with similarity to C. elegans dpy-30 gene product, GB

-2.2/-1.8/-1.4/-1.3

YGR076C

MRPL25

Mitochondrial ribosomal protein of the large subunit YMR26 (YmL25)

-2.2/-1.7/-1.6/-1.5

YBR024W

SCO2

Protein possibly involved in stability of Cox1p and Cox2p; homolog of Sco1p

-2.2/-1.7/-1.1/-1.5

YGR272C

 

Protein of unknown function

-2.2/-1.6/-1.1/1

YNL110C

 

Protein of unknown function

-2.2/-1.5/-1.1/-1.3

YKL109W

HAP4

Transcription factor with acidic activation domain, component of Hap2p-Hap3p-Hap4p-Hap5p complex involved in activation of CCAAT box-containing genes

-2.1/-1.7/-2.7/-3

YLR066W

SPC3

Subunit of signal peptidase complex

-2.1/-1.5/-1.5/-1.4

YLL062C

 

Protein of unknown function

-2/-1.6/-2.2/-2.4

YKL186C

MTR2

Protein involved in mRNA transport, has similarity to E. coli mbeA

-2/-1.6/-1.1/-1.4

YBR298C

MAL31

High affinity maltose/H+ symporter (maltose permease) member of the sugar permease family

-2/-1.6/-1.8/-2.2

YPR143W

 

Protein of unknown function

-2/-1.7/-1.4/-1.2

YDR041W

 

Mitochondrial ribosomal protein

-2/-1.5/-1.3/-1.2

YNL114C

 

Protein of unknown function, questionable ORF

-1.9/-1.5/-1.1/-1.2

YGR030C

POP6

Subunit of both RNase P and RNase MRP

-1.9/-1.6/-1/-1.1

YJR133W

XPT1

Xanthine phosphoribosyl transferase

-1.9/-1.5/-1.3/-1.1

YJR101W

 

Protein with similarity to superoxide dismutase

-1.9/-1.5/-1.1/-1.3

YPL158C

 

Protein of unknown function

-1.9/-1.5/1.3/1

YHR081W

 

Protein of unknown function

-1.9/-1.6/-1.5/-1.4

YKR083C

 

Protein with similarity to paramyosin

-1.9/-1.5/-1.3/-1.3

YBR201W

DER1

Protein involved in degradation of misfolded soluble proteins in the endoplasmic reticulum

-1.9/-1.5/-1.3/-1.3

YDR073W

SNF11

Component of SWI-SNF global transcription activator complex; acts to assist gene-specific activators through chromatin remodeling

-1.8/-1.5/-1.7/-1.7

YBL043W

ECM13

Protein possibly involved in cell wall structure or biosynthesis

-1.8/-1.5/1.1/-1.2