Irvine lab publications


  1. 1.Yamanaka YJ, Szeto GL, Gierahn TM, Forcier TL, Benedict KF, Brefo MSN, Lauffenburger DA, Irvine DJ, Love JC, “Cellular barcodes for efficiently profiling single-cell secretory responses by microengraving,” Anal. Chem. online (2012).

  2. 2.        DeMuth PC, Moon JJ, Suh H, Hammond PT, and Irvine DJ, “Releasable layer-by-layer assembly of stabilized lipid nanocapsules on microneedles for enhanced transcutaneous vaccine delivery,” ACS Nano in press.

  3. 3.  DeMuth PC, Garcia-Beltran WF, Ai-Ling ML, Hammond PT, and Irvine DJ, “Composite dissolving microneedles for coordinated control of antigen and adjuvant delivery kinetics in transcutaneous vaccination,” Adv. Funct. Mater. in press.

  4. 4.   Stephan MT, Stephan S, Bak SP, Chen J, and Irvine DJ, “Synapse-directed delivery of immunomodulators using T-cell-conjugated nanoparticles,” Biomaterials. 33(23) 5776-5787 (2012).

  5. 5.   Moon JJ, Huang B, and Irvine DJ, “Engineering nano- and micro-particles to tune immunity,” Advanced Materials. 24(28) 3724-3746 (2012).

  6. 6.     Moon JJ, Suh H, Li AV, Ockenhouse CF, Yadava A, and Irvine DJ, “Enhancing humoral responses to a malaria antigen with nanoparticle vaccines that expand Tfh cells and promote germinal center induction,” Proc. Natl. Acad. Sci., U.S.A. 109 1080-1085 (2012).

  7. 7.   Moon JJ, Suh H, Polhemus ME, Ockenhouse CF, Yadava A, and Irvine DJ, “Antigen-displaying lipid-enveloped PLGA nanoparticles as delivery agents for a Plasmodium vivax malaria vaccine,” PLoS ONE 7, e31472 (2012).

  8. 8.     Bershteyn A, Hanson MC, Crespo MP, Moon JJ, Li AV, Suh H, and Irvine DJ, “Robust IgG responses to nanograms of antigen using a biomimetic lipid-coated particle vaccine,” Journal of Controlled Release 157 3543-365 (2012).

  9. 9.     Metallo CM, Gameiro PA, Bell EL, Mattaini KR, Yang J, Hiller K, Jewell CM, Johnson ZR, Irvine DJ, Guarente L, Kelleher JK, Vander Heiden MG, Iliopoulos O, and Stephanopoulos G, “Reductive glutamine metabolism by IDH1 mediates lipogenesis under hypoxia,” Nature 481, 380–384 (2012).


  1. 10.  Jewell CM,  Jung JM, Atukorale PU, Carney RP, Stellacci F, and Irvine DJ, “Oligonucleotide delivery by cell-penetrating “striped” nanoparticles,” Angew. Chem. Intl. Ed. 50 12312-12315 (2011).

  2. 11.      Jewell CM, Bustamante Lopez SC, and Irvine DJ, “In situ engineering of the lymph node microenvironment via intranodal injection of adjuvant-releasing polymer particles,” Proc. Natl. Acad. Sci., U.S.A. 108(38) 15745-15750 (2011).  PMCID: PMC3179077

  3. 12.      Liu H, Kwong B, and Irvine DJ, “Membrane Anchored Immunostimulatory Oligonucleotides for in vivo Cell Modification and Localized Immunotherapy,” Angew. Chemie Intl. Ed. 50 7052-7055 (2011). PMCID: PMC3166645

  4. 13.      Kwong B, Liu H, and Irvine DJ, “Induction of potent anti-tumor responses while eliminating systemic side effects via liposome-anchored combinatorial immunotherapy,” Biomaterials 32(22) 5134-5147 (2011).

  5. 14.      De Temmerman M-L, Rejman J, Demeester J, Irvine DJ, Gander B, and De Smedt SC, “Particulate vaccines: on the quest for optimal delivery and immune response,” Drug Discovery Today 16 569-582 (2011).

  6. 15.      Wang Y and Irvine DJ, “Engineering chemoattractant gradients using chemokine-releasing polysaccharide microspheres,” Biomaterials 32(21) 4903-4913 (2011)PMCID: PMC3139910

  7. 16.      Yoo J-W, Irvine DJ, Discher DE, and Mitragotri S, “Bio-inspired, bioengineering and biomimetic drug delivery carriers,” Nature Reviews Drug Discovery 10(7) 521-535 (2011).

  8. 17.      Stephan MT and Irvine DJ, “Enhancing cell therapies from the outside in: Cell surface engineering using synthetic nanomaterials,” Nano Today 6 309-325 (2011). PMCID: PMC2935928

  9. 18.      Su X, Fricke J, Kavanagh D, and Irvine DJ, “In vitro and in vivo mRNA delivery using lipid-enveloped pH-responsive polymer nanoparticles,” Molecular Pharmaceutics 8(3) 774-787 (2011).

  10. 19.    Irvine, D. J. Drug delivery: One nanoparticle, one kill. Nature Materials 10, 342–343 (2011).

  11. 20.     Irvine DJ and Jewell CM, “Vaccine and Immunotherapy Delivery”, in Comprehensive Biomaterials, Ed. P. Ducheyne, Elsevier Press (2011).

  12. 21.    Martinez-Martin N, Fernandez-Arenas E, Cemerski A, Delgado P, Turner M, Neuser J, Irvine DJ, Huang B, Bustelo XR, Shaw A, and Alarcon B, “T cell receptor internalization from the immunological synapse is mediated by TC21 and RhoG GTPase-dependent phagocytosis,” Immunity 35(2) 208-222 (2011).

  13. 22.      Palmer MJ, Mahajan VS, Chen J, Irvine, DJ, and Lauffenburger DA, “Signaling thresholds govern heterogeneity in IL-7-receptor-mediated responses of naïve CD8+ T cells,” Immunology and Cell Biology 89(5) 581-594 (2011).  **Highlighted as an Outstanding Observation by the journal.

  14. 23.      Melvin AT, Welf ES, Wang Y, Irvine DJ, Haugh JM, “In chemotaxing fibroblasts, both high-fidelity and weakly biased cell movements track the localization of PI3K signaling,” Biophysical Journal 100(8) 1893-1901 (2011)**Highlighted as a Featured Article by the journal.

  15. 24.      Moon JJ, Suh H, Bershteyn A, Stephan MT, Liu H, Huang B, Sohail M, Luo S, Um SH, Chiu W, and Irvine DJ, “Interbilayer-crosslinked multilamellar vesicles for potent humoral and cellular immune responses,” Nature Materials 10(3) 243-251 (2011).

  16. 25.      Ackerman ME, Moldt B., Wyatt, RT, Dugast A, McAndrew E, Tsoukas S, Jost S, Berger CT, Sciaranghella G, Liu Q, Irvine DJ, Burton DR, and Alter G, “A robust, high-throughput assay to determine the phagocytic activity of clinical antibody samples,” Journal of Immunological Methods 366(1-2) 8-19 (2011).


  1. 26.        DeMuth P, Su X, Samuel RE, Hammond PT, and Irvine DJ, “Nano-Layered microneedles for transcutaneous delivery of polymer nanoparticles and plasmid DNA,” Advanced Materials, 22(43) 4851-4856 (2010).  (cover article)

  2. 27.         Stephan M, Moon JJ, Um SH, Bershteyn A, and Irvine DJ, “Therapeutic cell engineering with surface-conjugated synthetic nanoparticles,” Nature Medicine, 16(9) 1035-1041 (2010).

  3. 28.         Atala A, Irvine DJ, Moses M, and Shaunak S, “Wound healing versus regeneration: Role of the tissue environment in regenerative medicine,” MRS Bulletin 35(8) 597-606 (2010).

  4. 29.         Phee H, Dzhagalov I, Mollenauer M, Wang Y, Irvine DJ, Robey E, and Weiss A, “Regulation of thymocyte positive selection and motility by GIT2,” Nature Immunology 11(6) 503-11 (2010).

  5. 30.         Swiston AJ, Gilbert JB, Irvine DJ, Cohen RE, and Rubner MF, “Freely suspended cellular “backpacks” lead to cell self-assembly,” Biomacromolecules 11 1826-1832 (2010).


  1. 31.        Hori Y, Stern PJ, Hynes RO, and Irvine DJ, “Engulfing tumors with synthetic extracellular matrices for cancer immunotherapy,” Biomaterials 30 6757-6767 (2009).

  2. 32.         Su X, Kim BS, Kim SR, Hammond PT, and Irvine DJ, “Layer-by-Layer-Assembled multilayer films for transcutaneous drug and vaccine delivery,” ACS Nano 3(11) 3719-3729 (2009).

  3. 33.         Hu Y, Atukorale PU, Lu JJ, Moon JJ, Um SH, Cho EC, Wang Y, Chen J, and Irvine DJ, “Cytosolic delivery mediated via electrostatic surface binding of protein, virus, or siRNA cargos to pH-responsive core-shell gel particles,” Biomacromolecules,10 756-765 (2009).

  4. 34.         Maldonado RA, Soriano MA, Perdomo C, Sigrist K, Irvine DJ, Decker T, and Glimcher LH, “Control of T helper cell differentiation through cytokine receptor inclusion in the immunological synapse,” Journal of Experimental Medicine 206 877-892 (2009).

  5. 35.         Hori Y, Winans AM, and Irvine DJ, “Modular injectable matrices based on alginate solution/microsphere mixtures that gel in situ and co-deliver immunomodulatory factors,” Acta Biomaterialia 5 969-982 (2009).  PMCID: PMC3042245

  6. 36.         Miller AC, Bershteyn A, Tan W, Hammond PT, Cohen RE, and Irvine DJ, “Block copolymer micelles as nanocontainers for controlled release of proteins from biocompatible oil phases,” Biomacromolecules 10 732-741 (2009).


  1. 37.         Hori Y, Winans AM, Huang CC, Horrigan EM, and Irvine DJ, “Injectable dendritic cell-carrying alginate gels for immunization and immunotherapy,” Biomaterials 29 3671-3682 (2008).

  2. 38.        Swiston AJ, Cheng C, Um SH, Irvine DJ, Cohen RE, and Rubner MF, “Surface functionalization of living cells with multilayer patches,” Nano Letters 8 4446-4453 (2008).

  3. 39.        Verma A, Uzun O, Hu Y, Watson N, Chen S, Irvine DJ, and Stellacci F, “Cell penetrating nanoparticles: the role of ligand shell structure,” Nature Materials, 7 588-595 (2008). PMCID: PMC2684029

  4. 40.        Bershteyn A, Chaparro J, Yau R, Kim M, Reinherz E, Ferreira-Moita L, and Irvine DJ, “Polymer-Supported lipid shells, onions, and flowers,” Soft Matter 4 1787-1791 (2008).  PMCID: PMC2743563

  5. 41.        Miller AC, Bennett RD, Hammond, PT, Irvine DJ, and Cohen RE, “Functional nanocavity arrays via amphiphilic block copolymer thin films,” Macromolecules 41(5) 1739-1744 (2008).

  6. 42.        Irvine DJ, Stachowiak AN, and Y Hori, “Lymphoid tissue engineering: Invoking lymphoid tissue neogenesis in immunotherapy and models of immunity,” Seminars in Immunology 20 137-146 (2008).

  7. 43.        Stachowiak AN and Irvine DJ, “Inverse opal hydrogel-collagen composite scaffolds as a supportive microenvironment for immune cell migration,” Journal of Biomedical Materials Research 85A 815-828 (2008).

2007 and earlier

  1. 44.        Hu Y, Litwin T, Nagaraja AR, Kwong B, Katz J, Watson N, and Irvine DJ, “Cytosolic delivery of membrane-impermeable molecules in dendritic cells using pH-responsive core-shell nanoparticles,” Nano Letters, 7(10); 3056-3064 (2007). [Abstract]

  2. 45.        Irvine DJ, Doh J, and Huang B, “Patterned surfaces as tools to study ligand recognition and synapse formation by T cells,” Current Opinion in Immunology 19 463-469, (2007).

  3. 46.       Irvine DJ, Doh J, “Synthetic surfaces as artificial antigen presenting cells in the study of T cell receptor triggering and immunological synapse formation,” Seminars in Immunology 19 245-254 (2007).

  4. 47.       Stachowiak AN, Wang Y, Huang Y-C, and Irvine DJ, “Homeostatic lymphoid chemokines synergize with adhesion ligands to trigger T and B lymphocyte chemokinesis,” The Journal of Immunology, 177(4) 2340-2348 (2006). [full text]

  5. 48.        Doh J, and Irvine DJ, “Immunological Synapse Arrays: Patterned Protein Surfaces that Template Immunological Synapse Structure Formation in T Cells,” J. Doh and D.J. Irvine, Proceedings of the National Academy of Sciences, U.S.A., 103(15) 5700-5705 (2006). [full text]

  6. 49.        Kim H, Cohen RE, Hammond PT, and Irvine DJ, “Live Lymphocyte Arrays for Biosensing,” Advanced Functional Materials, 16 1313-1323 (2006). [Abstract]

  7. 50.        "Composition-tunable properties of amphiphilic comb copolymers containing protected methacrylic acid groups for multicomponent protein patterning," J.S. Katz, J. Doh, and D.J. Irvine, Langmuir 22 (1) 353-359 (2006).   [Abstract]  [PDF]

  8. 51.       Bennett, R.D., Hart, A.J., Miller, A.C., Hammond, P.T., Irvine, D.J., and R.E. Cohen, “Creating patterned carbon nanotube catalysts through the microcontact printing of block copolymer micellar thin films,” Langmuir 22(20) 8273-8276 (2006). [Abstract]

  9. 52.      "Strategies for controlling the planar arrangement of block copolymer micelles and inorganic nanoclusters," R.D. Bennette, A.C. Miller, N.T. Kohen, P.T. Hammond, D.J. Irvine, and R.E. Cohen, Macromolecules 38 (26) 10728-10735 (2005).   [Abstract]  [PDF]

  10. 53."Synthesis of protein-loaded hydrogel particles in an aqueous two-phase system for coincident antigen and CpG oligonucleotide delivery to antigen-presenting cells," S. Jain, W.T. Yap, and D.J. Irvine, Biomacromolecules 6 (5) 2590-2600 (2005).   [Abstract]  [PDF]

  11. 54."beta-amino ester polymers facilitate in vivo DNA transfection and adjuvant plasmid DNA immunization," J.R. Greenland, H.N. Liu, D. Berry, D.G. Anderson, W.K. Kim, D.J. Irvine, R. Langer, and N.L. Letvin, Molecular Therapy 12 (1) 164-170 (2005).   [Abstract]  [PDF]

  12. 55."Directed cell migration via chemoattractants released from degradable microspheres," X. Zhao, S. Jain, B. Larman, S. Gonzalez, and D.J. Irvine, Biomaterials 26 (24) 5048-5063 (2005).   [Abstract]  [PDF]

  13. 56."Bioactive hydrogels with an ordered cellular structure combine interconnected macroporosity and robust mechanical properties," A.N. Stachowiak, A. Bershteyn, and D.J. Irvine, Adv. Mater. 17 (4) 399 (2005).

  14. 57."A role for the immunological synapse in lineage commitment of CD4 lymphocytes," R.A. Maldonado, D.J. Irvine, R. Schreiber, and L.H. Glimcher, Nature 431 527-532 (2004).   [Abstract]  [PDF]

  15. 58."Photogenerated polyelectrolyte bilayers from an aqueous-processible photoresist for multiple protein patterning," J. Doh, and D.J. Irvine, J. Am. Chem. Soc., 126 (30) 9170-9171 (2004).  [Abstract]  [PDF]

  16. 59."CD4 enhances T cell sensitivity to antigen by coordinating Lck accumulation at the immunological synapse," Q.J. Li, A.R. Dinner, S.Y. Qi, D.J. Irvine, J.B. Huppa, M.M. Davis, and A.K. Chakraborty, Nature Immunology , 5(8) 791-799 (2004).  [Abstract]   [PDF]

  17. 60."T cell killing does not require the formation of a stable mature immunological synapse," M.A. Purbhoo, D.J. Irvine, J.B. Huppa , and M.M. Davis, Nature Immunology , 5 524-530 (2004).  [Abstract]   [PDF]

  18. 61."Large Area Two-Dimensional B Cell Arrays for Sensing and Cell-Sorting Applications," H. Kim, J. Doh, D.J. Irvine, R.E. Cohen, and P.T. Hammond, Biomacromolecules , 5 822-827 (2004).    [Abstract]   [PDF]

  19. 62."Function-specific variations in the immunological synapses formed by cytotoxic T cells," D.J. Irvine, Proc. Natl. Acad. Sci. USA 100 , 13739-13740 (2003).   [Abstract]   [PDF]

  20. 63."Engineering biomaterials for control of immune cell functions," D.J. Irvine, A. Stachowiak, and S. Jain, Materials Science Forum , 426-4 3213-3218 (2003).

  21. 64."Dynamics of cell surface molecules during T cell recognition," M.M.Davis, M. Krogsgaard, J.B.Huppa, C. Suman, M.A. Purbhoo, D.J. Irvine, L.C. Wu, and L. Ehrlich, Annual Review of Biochemistry , 72 712-742 (2003).   [Abstract]    [PDF]

  22. 65."Direct observation of ligand recognition by T cells," D.J. Irvine, M.A. Purbhoo, M. Krogsgaard, and M.M. Davis, Nature, 419(6909) 845-849 (2002).   [Abstract]   [PDF]

  23. 66."Rapid signaling-induced receptor reorientation in the formation of the immunological synapse," W. Moss, D.J. Irvine, M. Krummel, and M.M. Davis, Proc. Natl. Acad. Sci. USA 99, 15024-15029 (2002).   [Abstract]   [PDF]

  24. 67."Co-regulation of cell adhesion by nanoscale RGD organization and mechanical stimulus," Lily Y. Koo, Darrell J. Irvine, Anne M. Mayes, Douglas A. Lauffenburger, and Linda G. Griffith, J. Cell Sci. 115 1423-1433 (2002).    [Abstract]   [PDF]

  25. 68."Simulations of Cell-Surface Integrin Binding to Nanoscale-Clustered Adhesion Ligands," D.J. Irvine, K.-A. Hue, A.M. Mayes, and L.G. Griffith, Biophys. J., 82(1) 120-132 (2002).  [Abstract]   [PDF]

  26. 69."Nanoscale Clustering of RGD Peptides at Surfaces Using Comb Polymers. 2. Surface Segregation of Comb Polymers in Polylactide," D.J. Irvine, A.-V. Ruzette, A.M. Mayes, and L.G. Griffith, Biomacromolecules, 2(2) 545-556 (2001).   [Abstract]   [PDF]

  27. 70."Nanoscale Clustering of RGD Peptides at Surfaces Using Comb Polymers. 1. Synthesis and Characterization of Comb Thin Films," D.J. Irvine, A.M. Mayes, and L.G. Griffith, Biomacromolecules, 2(1) 85-94 (2001).  [Abstract]  [PDF]

  28. 71."Polymer Latexes for Cell-Resistant or Cell-Interactive Surfaces," P. Banerjee, D.J. Irvine, A.M. Mayes, and L.G. Griffith, J. Biomed. Mater. Res., 50(3) 331-339 (2000).  [Abstract]  [PDF]

  29. 72."Tailored Polymer Surfaces for Controlled Cell Interactions," A.M. Mayes, D.J. Irvine, and L.G. Griffith, Mater. Res. Soc. Symp. Proc., 530, 73-84 (1998).

  30. 73."Comparison of Tethered Star and Linear Poly(Ethylene Oxide) for Control of Biomaterials Surface Properties," D.J. Irvine, A.M. Mayes, S.K. Satija, J.G. Barker, S.J. Sofia-Allgor, and L.G. Griffith, Journal of Biomedical Materials Research, 40(3) 498-509 (1998). [Abstract]  [PDF]

  31. 74."Design of Biocompatible Surfaces," A.M. Mayes, D.G. Walton, D.J. Irvine, and L. Griffith, Amer. Chem. Soc. Proc. Div. Mat. Sci. Eng., 75, 78-79 (1996).

  32. 75."Self-Consistent Field Analysis of Grafted Star Polymers," D.J. Irvine, A.M. Mayes, and L. Griffith-Cima, Macromolecules, 29(18) 6037-6043 (1996). [Abstract]  [PDF]

  33. 76."Computer Simulations of Self-Assembling Comb copolymers," D.J. Irvine, D. Gersappe, and A.C.Balazs, Langmuir, 11(10) 3848-3855 (1995). [Abstract]  [PDF]

  34. 77."The Use of Graft Copolymers to Bind Immiscible Blends," D. Gersappe, D. Irvine, A.C. Balazs, Y. Liu, J. Sokolov, M. Rafailovich, S. Schwarz, and D.G. Peiffer, Science, 265(5175) 1072-1074 (1994). [Abstract]  [PDF]

  35. 78."Contrasting the Compatibilizing Activities of Comb and Linear Copolymers," D. Gersappe, P.K. Harm, D. Irvine, and A.C. Balazs, Macromolecules, 27 (3) 720-724 (1994). [Abstract]  [PDF]