Enzymes



General Enzymes –35 backroom   dd 5-2-2002 AvdF

Data Sheets are in the black binder on top of freezer

All New England Biolab unless indicated otherwise.

 

Restriction Enzymes

 

AatII     GACGT/C              4    37   R0117S

AluI      AG/CT                2    37   R0137S

BamH1     G/GATCC        BamH1 /BSA 37   R0136L

BbsI      GAAGAC(N2)/          2    37   R0539S

BglI      GCCNNNN/NGGC         3    37   R0143S

BglII     A/GATCT              3    37   R0144S

BlpI      GC/TNAGC             4    37   R0585S

BstEII    G/GTNACC             3    60   R0162S

Bsu36I    CC/TNAGG       3/BSA     37   R0524S

ClaI      AT/CGAT   dam  4/BSA     37   R0197S

DpnI      GA/TC                4    37   R0176S

DraIII    CACNNN/GTG     3/BSA     37   R0510S

EcoRI     G/AATTC        EcoRI     37   R0101L

EcoRV     GAT/ATC        3/BSA     37   R0195S

HincII    GTPy/PuAC      3/BSA     37   R0103S

HindIII   A/AGCTT              2    37   R0104L

KpnI      GGTAC/C        1/BSA     37   R0142S

NcoI      C/CATGG              4    37   R0193S

NdeI      CA/TATG              4    37   R0111S

NheI      G/CTAGC        2/BSA     37   R0131S

NotI      GC/GGCCGC      3/BSA     37   R0189L

PstI      CTGCA/G        3/BSA     37   R0140S

PvuI      CGAT/CG        3/BSA     37   R0150S

PvuII     CAG/CTG              2    37   R0151S

SacI      GAGCT/C        1/BSA     37   R0156S

SacII     CCGC/GG              4    37   R0157S

SalI      G/TCGAC        Sal/BSA   37   R0138S

ScaI      AGT/ACT        Sca       37   R0122S

SmaI      CCC/GGG              4    25   R0141S

SnaBI     TAC/GTA        4/BSA     37   R0130S

SpeI      A/CTAGT        2/BSA     37   R0133S

SwaI      ATTT/AAAT      3/BSA     25   R0604S

XbaI      T/CTAGA        2/BSA     37   R0145L

XhoI      C/TCGAG        2/BSA     37   R0146L

XmaI      C/CCGGG        4/BSA     37   R0180S

XmnI      GAANN/NNTTC    2/BSA     37   R0194S        

 

 

 

Miscellaneous Enzymes (5-2-2002 AvdF)

 

Mung Bean nuclease             Mung Bean Buf        M0250S

T4 DNA Ligase                  T4 lig buf           M0202L

T4 polynucleotide kinase       T4 polynucl buf      M0201S

 

Polymerases

Deep VentR DNA Polymerase     ThermoPol buf       M0258L

DNA Polymerase I (E coli)      EcolPol             M0209L

DNA polymerase I Large fragment (Klenow) EcoPol buf M0210S

T4 DNA Polymerase                   T4 Pol Buf     M0203S

 

TakaRa Taq                               (Takara R001B)

HotStar Taq                              (Qiagen 203205)

ProofStar DNA Polymerase (proofread PCR) (Qiagen 202203)

PWO DNA polymerase                       (Roche 1-644955)

Platinum DNA Polymerase             (Life-Gibco 11304-11)

 

RNA Work

SuperscriptII RNAseH- reverse transcriptase   5x 1rst strand buffer (Invitrogen 18064-014)

 

T3-RNA Polymerase  transcription buf, DTT (Promega P2083)

T7 RNA Polymerase, transcription buff, DTT (Promega, P2075)

SP6 RNA Polymerase transcrip[tion mbuffer, DTT(Promega P1085)

RNAsin                                        (Promega N211B)

 

 

BP Ladders

 

100 bp ladder        Amersham 27 4001-01

Hyperladder I        Bioline Bio-33026

 

(PhiX HaeIII         302-6L)

(pBR322 MspI         303-11)

 

 

Protein MW markers

 

Kaleidoscope prestained markers          (Biorad 161-0324)