Professor Dewey is a professor in the Mechanical Engineering Department and holds joint appointments in the Division of Biological Engineering and the Division of Health Sciences and Technology. He is
also Co-Director of the International Consortium for
Medical Imaging Technology (ICMIT)
goal is the development and promotion of advanced technology for medical
Professor Dewey's research interests include fluids
mechanics, cell dynamics and modeling, biomedical imaging, medical
informatics, and instrumentation.
effects of mechanical forces on living cells
The primary medical
problem that the Dewey research group investigates is
atherosclerosis. The lumen of the arterial wall is lined with
endothelial cells that respond to the mechanical forces of blood
flowing through the arteries. The endothelium protects the artery wall from
inflammatory reactions that result in atherosclerosis. Our lab was
the first to demonstrate the time-course with which endothelial cells
reorganize and reorient in the direction of fluid flow, and we
continue to use microscopy to probe the fundamental mechanisms of
this response. The mechanical structure of vascular endothelial
cells depends on a network of polymerized actin molecules, and we
use fluorescent dyes to track individual actin molecules as they
diffuse through the cell.
In our in vitro model system,
application of fluid shear force that is comparable to vascular
blood flow in vivo induces a major transient decrease in polymerization. We
believe that this alteration is correlated with the observed realignment of
the cells in the flow direction. Measurements of cell
motility and intercellular gap junction proteins are extending our understanding
of the interaction process. This research is a collaborative effort with
Prof. John Hartwig at the Brigham and Women's
Hospital, Harvard Medical School.
As part of this project, we are collaborating with Prof. James
McGrath at the University of Rochester to develop a computation
model of actin polymerization. The ability to peer inside individual
cells with modern fluorescence techniques has powered a profound
change in our quantitative understanding of cellular biological
function. For example, we reconstruct 3-dimensional images from
stereo image pairs taken with a scanning electron microscopy (SEM).
These images allow us to see the interaction of individual
structural features within the cells at the molecular level – e.g,
interactions between polymerized actin filaments and the actin
binding protein filamin-A.
Integration of experimental data from
A second major program within the lab is the
development of robust information systems for biological data. Our
goal is to develop a common technology that will capture data from
all of the major experimental systems in a single ontological
We have implemented this approach for gel
electrophoresis, microarrays and fluorescence-activated cell
sorting, demonstrating that data from these diverse sources can be
stored and interrogated within a single database or an integrated
network of databases. Currently we are incorporating mass
spectrometry and optical microscopy into this system. This
cross-disciplinary data integration effort is coordinated by the
Interoperable Informatics Infrastructure Consortium (I3C), which was
organized to promote and develop methodologies for data and tool
interoperability in the life sciences, based on open and public
specifications and protocols.
This work is based on a model
that was pioneered by the International Consortium for Medical Information Technology (ICMIT), which we founded to develop and
implement international data standards in medical imaging technologies
such as magnetic resonance imaging (MRI), computerized axial tomography
(CT) and X-rays. The Co-Director of the ICMIT is
Prof. Richard Kitney of Imperial College in London.
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Actin Dynamics in Vascular
Medical information infrastructure
Method of Indexing and Implicitly Discovering Document Logical Structures
A comprehensive physiome of the heart
Actin Dynamics in Motile Cells
Biomedical image analysis
Effects of fluid forces on vascular endothelium
Studying the Nanostructure of the Actin Cytoskeleton
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Post Doctoral Fellow:
Chan Young Park
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Matthieu Ferrant (visiting
student from UCL, Belgium)
(visting scholar from the Fraunhofer Institute in Berlin, Germany.
Shixin (Jason) Zhang
Yoshihiro Yasumuro (visiting student from Naist, Japan)
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Measurement and Instrumentation
2.771J / BE.453J / HST.958J -
Biomedical Information Technology
2.996 - Advance Topics in Mechanical
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Recent Selected Publication:
1. Satcher RL JR, Bussolari SR, Gimbrone MA JR, Dewey CF
JR. The Distribution of Fluid Forces on Model Arterial Endothelium
Model Arterial Endothelium Using Computational Fluid Dynamics.
J. Biomechanical Engineering 1992;114:309-316.
2. Tardy Y, McGrath JL, Hartwig JH, Dewey CF JR. Interpreting
Photoactivated Fluorescence Microscopy Measurements of Steady-State
Actin Dynamics. Biophys. J. 1995;69:1674-1682.
3. Dewey CF JR, Thomas JD, Kunt M, Hunter IW. Prospects for
Telediagnosis Using Ultrasound. Telemedicine Journal 1996;2:87-100.
4. Dewey CF JR. Virtual Files: The Key to Managing Medical Images (Abstract).
Ann. Proc. Fall Meeting of Biomedical Engineering Society; 1996.
5. Tardy Y, Resnick N, Nagel T, Gimbrone MA JR, Dewey CF
JR. Shear Stress Gradients Remodel Endothelial Monolayers in Vitro
Via a Cell Proliferation-Migration-Loss Cycle. Arterios., Thromb.,
& Vasc., Biol. 1997;17:3102-3106.
6. Satcher RL JR, Dewey CF JR, Hartwig JH. Mechanical
Remodeling of the Endothelial Surface and Actin Cytoskeleton Induced
by Fluid Flow. Microcirculation 1997; 4:439-453.
7. Dewey CF JR. Extending Dicom Databases to New Imaging Modalities (Abstract).
Proc. World Congress on Medical Physics and Biomedical Eng.; 1997
Sept. 14-19, 1997; Nice, France.
8. Dewey CF
JR. An Object-Relational Architecture for a Dicom Medical Image
Archive (Abstract). Proc. World Congress on Medical Physics and
Biomedical Eng.; 1997 Sept. 14-19, 1997; Nice, France.
9. Dao N, Dewey CF JR. Databasing Strategy for the Human Physiome,
(Abstract). Ann Biomed. Eng'g 1998;26 (Suppl. 1), S-13.
10. Dao N, Dewey CF JR. Design and Prototype of a Database for
Medical Images, (Abstract). Ann. Biomed. Eng'g 1998;26 (Suppl.
11. McGrath JL, Tardy Y, Dewey CF JR, Meister JJ, Hartwig JH.
Simultaneous Measurements of Actin Filament Turnover, Filament
Fraction, and Monomer Diffusion in Endothelial Cells. Biophys.
12. Dewey CF JR, Kitney RI. Creating DICOM-Enabled Clinical
Systems with Robust Image-Querying Capabilities. Proc. Towards
An Electronic Patient Record '98; C. P. Waegemann, editor. 1998
May 9-15, 1998; San Antonio, TX. Medical Records Institute.
13. McGrath JL, Hartwig J, Tardy Y, Dewey CF JR. Measuring
Actin Dynamics in Endothelial Cells. Microscopy Research and Technique
14. Nagel T, Resnick R, Dewey CF JR, Gimbrone MA
JR. Vascular Endothelial Cells Respond to Spatial Gradients in
Fluid Shear Stress by Enhanced Activation of Transcription Factors,
Arteriosclerosis, Thrombosis, and Vasc. Biol. 19, 1825-1834 (1999).
15. Dao N., McCormick P J, Dewey CF JR. The human
physiome as an information environment. Annals of Biomedical Engineering
28, 1032-1042 (2000).
16. Cheng Y, Hartemink C, Hartwig JH, Dewey CF JR. Three Dimensional
Reconstruction of the Cell Cytoskeleton from Stereo Images. J.
Biomechanics 33, 105-113 (2000).
17. McGrath JL, Osborn EA, Dewey
CF Jr, Tardy YS, Hartwig JH. Regulation of the actin cycle in vivo
by actin filament severing. Proc. Nat. Acad. Sciences 97, 6532-6537
18. Dewey CF JR, Fu B, Zhang S, Dao N, Chuang
W, Li Z. An information architecture for physiological models,
clients, and databases. Proc. IEEE-EMBS European Conference on
Medical and Biological Systems, October, 2001, Istanbul.
19. Fillit H et al. Barriers to Drug Discovery and Development
for Alzheimer Disease. Alzheimer Disease and Associated Disorders
16, S1-S8 (2002).
20. Dewey CF JR. Haemodynamic flow: symmetry and synthesis.
Biorheology 39, 541-549 (2002).
21. Dewey CF
JR, Zhang SA. Unique Opportunity in Biological Information Object Standards. Invited talk, 2003
World Congress on Medical Physics and Biomedical Engineering, August, 2003,
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