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Publications

 Research articles 2009-present

Computational design of a red fluorophore ligase for site-specific protein labeling in living cells.
PNAS 2014, accepted
D. S. Liu, L. G. Nivon, F. Richter, P. J. Goldman, T. J. Deerinck, J. Z. Yao, D. Richardson, W. S. Phipps, A. Z. Ye, M. H. Ellisman, C. L. Drennan, D. Baker, and A. Y. Ting.

Proteomic mapping of the human mitochondrial intermembrane space in live cells via ratiometric APEX tagging.
Molecular Cell 2014, advance online publication.
V. Hung, P. Zou, H.-W. Rhee, N. D. Udeshi, V. Cracan, T. Svinkina, S. A. Carr, V. K. Mootha, and A. Y. Ting.



Site-specific protein labeling using PRIME and chelation-assisted click chemistry.
Nature Protocols 2013, 8, 1620-1634.
C. Uttamapinant, M. I. Sanchez, D. S. Liu, J. Z. Yao, K. A. White, S. Grecian, S. Clark, K. R. Gee, and A. Y. Ting.



Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging.
Science 2013, 339, 1328-1331.
Highlight in Nature Methods
H.-W. Rhee, P. Zou, N. D. Udeshi, J. D. Martell, V. K. Mootha, S. A. Carr, and A. Y. Ting.



Imaging trans-cellular neurexin-neuroligin interactions by enzymatic probe ligation.
PLoS One 2013, 8, e52823.
D. S. Liu, K. H. Loh, S. S. Lam, K. A. White, and A. Y. Ting.



Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.
ACS Nano 2012, 6, 11080-11087.
D. S. Liu, W. S. Phipps, K. H. Loh, M. Howarth, and A. Y. Ting.



Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy.
Nature Biotechnology 2012, 30, 1143-1148.
Highlight in Nature Methods
J. D. Martell, T. J. Deerinck, Y. Sancak, T. L. Poulos, V. K. Mootha, G. E. Sosinsky, M. H. Ellisman, and A. Y. Ting.



Fast, cell-compatible click chemistry with copper-chelating azides for biomolecular labeling.
Angewandte Chemie International Edition 2012, 51, 5852-5856.
Highlight in Nature Chemical Biology
C. Uttamapinant, A. Tangpeerachaikul, S. Grecian, S. Clarke, U. Singh, P. Slade, K. R. Gee, and A. Y. Ting.



Site-specific protein modification using lipoic acid ligase and bis-aryl hydrazone formation.
ChemBioChem 2012, 13, 888-894.
J. D. Cohen, P. Zou, and A. Y. Ting.



Fluorophore targeting to cellular proteins via enzyme-mediated azide ligation and strain-promoted cycloaddition.
Journal of the American Chemical Society 2012, 134, 3720-3728.
J. Z. Yao, C. Uttamapinant, A. Poloukhtine, J. M. Baskin, J. A. Codelli, E. M. Sletten, C. R. Bertozzi, V. V. Popik, and A. Y. Ting.



Diels-Alder cycloaddition for fluorophore targeting to specific proteins inside living cells.
Journal of the American Chemical Society 2012, 134, 792-795.
D. S. Liu, A. Tangpeerachaikul, R. Selvaraj, M. T. Taylor, J. M. Fox, and A. Y. Ting.



Imaging protein-protein interactions inside living cells via interaction-dependent fluorophore ligation.
Journal of the American Chemical Society 2011, 133, 19769-19776.
S. A. Slavoff, D. S. Liu, J. D. Cohen, and A. Y. Ting.



Structure-guided engineering of a Pacific Blue fluorophore ligase for specific protein imaging in living cells.
Biochemistry 2011, 50, 8221-8225.
J. D. Cohen, S. Thompson, and A. Y. Ting.



Imaging LDL receptor oligomerization during endocytosis using a co-internalization assay.
ACS Chemical Biology 2011, 6, 308-313.
P. Zou and A. Y. Ting.



Synthesis of 7-aminocoumarin via Buchwald-Hartwig cross coupling for specific protein labeling in living cells.
ChemBioChem 2011, 12, 65-70.
X. Jin, C. Uttamapinant, and A. Y. Ting.



A fluorophore ligase for site-specific protein labeling inside living cells.
Proceedings of the National Academy of Sciences 2010, 107, 10914-10919.
C. Uttamapinant, K. A. White, H. Baruah, S. Thompson, M. Fernández-Suárez, S. Puthenveetil, and A. Y. Ting.



Yeast display evolution of a kinetically-efficient 13-amino acid substrate for lipoic acid ligase.
Journal of the American Chemical Society 2009, 131, 16430-16438.
S. Puthenveetil, D. S. Liu, K. A. White, S. Thompson, and A. Y. Ting.


 Research articles 2008-2004

An engineered aryl azide ligase for site-specific mapping of protein-protein interactions through photocrosslinking.
Angewandte Chemie International Edition 2008, 47, 7018-7021.
H. Baruah, S. Puthenveetil, Y.-A. Choi, S. Shah, and A. Y. Ting.



Protein-protein interaction detection in vitro and in cells by proximity biotinylation.
Journal of the American Chemical Society 2008, 130, 9251-9253.
M. Fernández-Suárez, T. S. Chen, and A. Y. Ting.



Monovalent, reduced-size quantum dots for imaging receptors on living cells.
Nature Methods 2008, 5, 397-399.
M. Howarth, W. Liu, S. Puthenveetil, Y. Zheng, L. F. Marshall, M. M. Schmidt, K. D. Wittrup, M. G. Bawendi, and A. Y. Ting.



Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin.
Nature Protocols 2008, 3, 534-545.
M. Howarth and A. Y. Ting.



Compact biocompatible quantum dots functionalized for cellular imaging.
Journal of the American Chemical Society 2008, 130, 1274-1284.
W. Liu, M. Howarth, A. B. Greytak, Y. Zheng, D. G. Nocera, A. Y. Ting, and M. G. Bawendi.



Expanding the substrate tolerance of biotin ligase through exploration of enzymes from diverse species.
Journal of the American Chemical Society 2008, 130, 1160-1162.
S. A. Slavoff, I. Chen, Y.-A. Choi, and A. Y. Ting.



Redirecting lipoic acid ligase for cell surface protein labeling with small-molecule probes.
Nature Biotechnology 2007, 25, 1483-1487.
M. Fernández-Suárez, H. Baruah, L. Martínez-Hernández, K. T. Xie, J. M. Baskin, C. R. Bertozzi, and A. Y. Ting.



Phage display evolution of a peptide substrate for yeast biotin ligase and application to two-color quantum dot labeling of cell surface proteins.
Journal of the American Chemical Society 2007, 129, 6619-6625.
I. Chen, Y.-A. Choi, and
A. Y. Ting.



Synthesis of a ketone analog of biotin via the intramolecular Pauson-Khand reaction.
Organic Letters 2006, 8, 4593-4595.
E. McNeill, I. Chen, and
A. Y. Ting.



Transglutaminase-catalyzed site-specific conjugation of small-molecule probes to proteins in vitro and on the surface of living cells.
Journal of the American Chemical Society 2006, 128, 4542-4543.
C.-W. Lin and
A. Y. Ting.



A monovalent streptavidin with a single femtomolar biotin binding site.
Nature Methods 2006, 3, 267-273.
M. Howarth,
D. J.-F. Chinnapen, K. Gerrow, P. C. Dorrestein, M. R. Grandy, N. L. Kelleher, A. El-Husseini, and A. Y. Ting.



Targeting quantum dots to surface proteins in living cells with biotin ligase.
Proceedings of the National Academy of Sciences 2005, 102, 7583-7588.
M. Howarth, K. Takao, Y. Hayashi, and A. Y. Ting.



Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase.
Nature Methods 2005, 2, 99-104.
I. Chen, M. Howarth, W. Lin, and A. Y. Ting.



A genetically encoded fluorescent reporter of histone phosphorylation in living cells.
Angewandte Chemie International Edition 2004, 43, 2940-2943.
C.-W. Lin and A. Y. Ting.



Genetically encoded fluorescent reporters of histone methylation in living cells.
Journal of the American Chemical Society 2004, 126, 5982-5983.
C.-W. Lin, C. Y. Jao, and A. Y. Ting.


 Reviews

Fluorescent probes for super-resolution imaging in living cells.
Nature Reviews Molecular Cell Biology 2008, 9, 929-943.
M. Fernández-Suárez and A. Y. Ting.



Next-generation optical technologies for illuminating genetically targeted brain circuits.

Journal of Neuroscience 2006, 26, 10380-10386.
K. Deisseroth, G. Feng, A. K. Majewska, G. Miesenbock, A. Y. Ting and M. J. Schnitzer.



Giving cells a new sugar-coating.
Nature Chemical Biology 2006, 2, 127-128.
M. Howarth and A. Y. Ting.



Site-specific labeling of proteins with small molecules in live cells.
Current Opinion in Biotechnology 2005, 16, 35-40.
I. Chen and A. Y. Ting.



Creating new fluorescent probes for cell biology.
Nature Reviews Molecular Cell Biology 2002, 3, 906-918.
J. Zhang, R. E. Campbell, A. Y. Ting and R. Y. Tsien.



Decoding NF-kB signaling.
Science 2002, 298, 1189-1190.
A. Y. Ting and D. Endy.