Christopher E. Carr, Sc.D.

Research Scientist

 

Current Efforts


1. High-throughput microscopy screen to identify genetic regulators of fat and body size in the worm C. elegans (Alex Soukas lab)


  1. Hundreds of thousands of images from screen of all 20,000 genes in the worm.

  2. MATLAB-based image analysis pipeline.

  3. Working towards cloud-based analysis.


2. New mathematical model of aging that generates key predictions such as when aging begins and how shapes of survival or mortality curves relate to the underlying biological process of aging.

High Throughput Screening yields insights into metabolism and aging through discovery science and pathway mapping.

Publications Based on High-Throughput Image Analysis


  1. Pino E, Webster C, Carr CE, and Soukas AA. Biochemical and High-Throughput Microscopic Assessment of Fat Mass in Caenorhabditis elegans. JoVE (accepted/In Press Aug 2012).

  2. Shore DE, Carr CE, Ruvkun G (2012) Induction of Cytoprotective Pathways Is Central to the Extension of Lifespan Conferred by Multiple Longevity Pathways. PLoS Genet 8(7): e1002792. doi:10.1371/journal.pgen.1002792

  3. O’Rourke E, Soukas A, Carr CE, and Ruvkun G. C. elegans Major Fats are stored in vesicles distinct from lysosome-related organelles. Cell Metabolism 4 Nov 2009 10(5):430-435; doi:10.1016/j.cmet.2009.10.002.

  4. Soukas AA, Kane EA, Carr CE, Melo JA, and Ruvkun G. Caenorhabditis elegans Rictor/TORC2 regulates fat metabolism and lifespan in a diet-dependent manner. Genes & Development (2009) 23:496-511; doi: 10.1101/gad.1775409


Prior work: Demography of mortality and movement


Identified genes required for longevity in long-lived worms (Andy Samuelson lab). Demonstrated decline in movement that parallels age-related increase in mortality: Samuelson AV, Carr CE, and Ruvkun G. Gene activities that mediate increased lifespan of C. elegans insulin-like signaling mutants. Genes & Development 2007 Nov 15; 21(22):2976. doi:10.1101/gad.1588907

Mathematical modeling of aging and demography explains prior results and generates new hypotheses and predictions.