The DIC Wang Research Group

Our present research is in the areas of recombinant protein production using Escherichia coli to increase protein expression by engineering and genetic manipulations. These include the use of magnetically coated fluorocarbons to increase oxygen transfer and two-phase aqueous micellar systems metal chelate affinity purification of proteins. We are also performing studies in animal cell culture with emphasis in the increased production of recombinant proteins and monoclonal antibodies. These include the understanding of cell death using proteomics to profile the different cell populations. In our animal cell culture research, we are active to increase the quality of therapeutic proteins. These include using genetic means to increase sialic acid content of recombinant glycoproteins and reduction on the loss of sialic acid during productions. Lastly, we have a program in the stabilization of proteins using quantum mechanics to simulate various events leading to protein inactivation. Our past research has been on new concepts in bioreactor design and operations, oxygen transfer in fermentors, fluid shear damage of animal cells, enzyme technology, membrane filtration and renewable resource utilization.

The Daniel I.C. Wang Group Members

Current research includes:

  • Effect of Culture Temperature on Recombinant IFN-γ Production and Quality (Steve Fox)
  • Transcriptional Profiling of Reduced Temperature on Recombinant Protein Production (Hong Kiat Tan)
  • Engineering of Chinese Hamster Ovary Cells for Improved Protein Quality (Frederyk Ngantung)
  • Purification of Recombinant Proteins with Magnetic Nanoclusters (Andre Ditsch)
  • Oxygen Transfer Enhancement to Cell Cultures by Use of Magnetic Fluids (Bernat Olle)
  • Affinity-Enhanced Protein Partitioning in Decyl β-D-Glucopyranoside Two-Phase Aqueous Micellar Systems (Henry Lam)
  • Genetic Manipulations to Increase Glycoprotein Sialic Acid Content (Niki Wong)
  • Molecular Dynamic Modeling of Protein Aggregation (Bin Pan)
  • Clonal Variations in Recombinant Mammalian Cells (Say Kong Ng)
  • Glyco-Chip for Real-time and On-line Monitoring of Glycoproteins (Sohan Patel)
  • Cell Arrest for Increased Recombinant Protein Production in Mammalian Cells (David McClain)