LOCUS T7.1-alpha_as_built 8750 bp DNA PHG 10-OCT-2004 DEFINITION Sequence of section alpha of T7.1 (Revision 1) as constructed by the Endy Group. ACCESSION # VERSION # GI:# KEYWORDS Bacteriophage; T7; refactored; synthetic; engineered; parts. SOURCE Endy Group, MIT. http://web.mit.edu/endy/ ORGANISM Enterobacteria phage T7 Viruses; dsDNA viruses, no RNA stage; Caudovirales; Podoviridae; T7-like viruses. REFERENCE 1 (bases 1 to 8750) AUTHORS Chan,L., Kosuri,S. and Endy,D. TITLE Refactoring Bacteriophage T7 JOURNAL MEDLINE REFERENCE 2 (bases 1 to 8750, partial) AUTHORS Dunn,J.J. and Studier,F.W. TITLE Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements JOURNAL J. Mol. Biol. 166 (4), 477-535 (1983) MEDLINE 83241725 REMARK complete genome REFERENCE 3 (bases 1 to 8750, partial) AUTHORS Dunn,J.J. and Studier,F.W. TITLE Direct Submission JOURNAL Submitted (23-NOV-1993) Dunn J.J REMARK complete genome COMMENT This is the DNA sequence of the "as-built" section alpha of T7.1 (ACCESSION#). Differences between the design and as-built sequence are annotated here. When combined with the remainder of the wild-type genome (8312..39938), the resulting chimeric genome codes for a viable bacteriophage. The chimera of section alpha (this section) with beta (ACCESSION#) and the remaining wild-type genome (11516..39938) also codes for a viable bacteriphage. COMMENT The design of T7.1 does not intentionally include any genetic functions different from those found on wild-type T7. Based on initial laboratory characterization, Chan, Kosuri, and Endy assert that the same precautions and safety measures that apply to wild-type T7 should also apply to T7.1. FEATURES Location/Qualifiers promoter complement(210..272) /standard_name="A0" /citation=[6] /citation=[1] /citation=[3] /citation=[5] /function="unknown; determined by in vitro transcription" /evidence=experimental /label=A0 /note="E. coli promoter A0 (leftward)" -10_signal complement(231..236) /standard_name="-10_A0" /label=-10_A0 -35_signal complement(255..259) /standard_name="-35_A0" /label=-35_A0 promoter 385..419 /standard_name="phiOL" /function="Replication promoter and Origin of Replication" /evidence=experimental /label=phiOL /note="T7 promoter phiOL -- Thought to be a part off the replication origin. The promoter can be utilized in restriction fragment of T7 DNA and may indeed direct transcription of the entire early region in vitro. There is no evidence that it directs transcription in vivo. Differs from the 23bp concensus sequence at two positions (-11 and +1)." misc_structure 412..412 /standard_name="S_phiOL" /function="putative mRNA start site" /label=S_phiOL promoter 462..524 /standard_name="A1" /citation=[2] /citation=[3] /function="E. coli promoter A1" /label=A1 /note="E. coli promoter A1. First E.coli prommoter primarily for transcription of T7 RNA polymerase and host shutdown mechanisms" misc_structure 466..466 /standard_name="-45_A1" /function="conserved -45 base of A1 promoter" /label=-45_A1 -35_signal 475..479 /standard_name="-35_A1" /label=-35_A1 -10_signal 498..503 /standard_name="-10_A1" /label=-10_A1 misc_structure 511..511 /standard_name="S_A1" /function="A1 mRNA start" /label=S_A1 promoter 589..652 /standard_name="A2" /citation=[2] /citation=[3] /function="E. coli promoter A2" /evidence=experimental /label=A2 /note="Second E. coli promoter primarily for transcription of T7 RNA polymerase and host shutdown mechanisms" misc_structure 593..593 /standard_name="-45_A2" /function="-45 conserved base" /label=-45_A2 -35_signal 602..606 /standard_name="-35_A2" /label=-35_A2 -10_signal 626..631 /standard_name="-10_A2" /label=-10_A2 misc_structure 639..639 /standard_name="S_A2" /function="A2 mRNA start site" /label=S_A2 promoter 714..776 /standard_name="A3" /citation=[2] /citation=[3] /function="E. coli promoter A3" /label=A3 /note="Third E.coli promoter primarily for transcription of T7 RNA polymerase and host shutdown mechanisms" misc_structure 718..718 /standard_name="-45_A3" /function="conserved -45 base" /label=-45_A3 -35_signal 727..731 /standard_name="-35_A3" /label=-35_A3 -10_signal 750..755 /standard_name="-10_A3" /label=-10_A3 misc_structure 763..763 /standard_name="S_A3" /function="mRNA start" /label=S_A3 stem_loop 866..914 /standard_name="RSL_R0.3" /label=RSL_R0.3 misc_structure 904..904 /standard_name="C_R0.3" /function="RNase III cut site" /label=C_R0.3 repeat_region 175..338 /label=SRL repeat_region 1..160 /label=TR misc_feature 164..170 /label=D1-L misc_feature 338..344 /label=D1-R misc_feature 431..436 /label=D2-R misc_feature 448..453 /label=D3-L misc_feature 542..547 /label=D3-R misc_feature 914..919 /label=D4-R misc_feature 920..925 /label=D5-L misc_feature 572..577 /label=D4-L misc_feature 1566..1571 /label=D7-L misc_feature 1624..1629 /label=U2 misc_feature 1730..1735 /label=D7-R misc_feature 1733..1738 /label=D8-L misc_feature 2095..2100 /label=D8-R misc_feature 2099..2105 /label=D9-L misc_feature 3208..3214 /label=D9-R misc_feature 3312..3317 /label=D10-R misc_feature 3315..3320 /label=D11-L misc_feature 5996..6001 /label=D11-R misc_feature 6149..6156 /label=D12-R misc_feature 6171..6176 /label=D13-L misc_feature 6325..6330 /label=D13-R misc_feature 6329..6335 /label=D14-L misc_feature 6619..6625 /label=D14-R misc_feature 6622..6628 /label=D15-L misc_feature 6711..6717 /label=D15-R misc_feature 6715..6720 /label=D16-L misc_feature 7820..7825 /label=D16-R misc_feature 7823..7828 /label=D17-L misc_feature 7877..7882 /label=D17-R misc_feature 7881..7886 /label=D18-L misc_feature 8061..8066 /label=D18-R misc_feature 8065..8070 /label=D19-L misc_feature 8105..8110 /label=D19-R misc_feature 8109..8114 /label=D20-L misc_feature 8231..8236 /label=D20-R misc_feature 8236..8241 /label=D21-L misc_feature 8277..8282 /label=D21-R misc_feature 8280..8286 /label=D22-L misc_feature 8572..8577 /label=D22-R misc_feature 8578..8583 /label=D23-L N_region 8745..8750 /label=N-BclI misc_feature 6559..6564 /label=U3 misc_feature 3215..3220 /label=D10-L misc_feature 6002..6009 /label=D12-L promoter 8071..8104 /label=phi1.5 promoter 8242..8276 /label=phi1.6 misc_feature 8186..8192 /label=U4 misc_feature 345..350 /label=D2-L misc_feature 1298..1303 /label=D5-R misc_feature 1304..1310 /label=D6-L misc_feature 1500..1506 /label=D6-R primer 454..487 /label=A1F primer complement(525..541) /label=A1R misc_difference 164..170 /label=D1L\deletion /note="DiL was never implemented" misc_difference 338..344 /label=D1R\deletion /note="D1R was never implemented" misc_difference 1311..1317 /label=extra\site misc_difference 6611..6617 /label=extra\site misc_difference 8115..8120 /label=extra\site misc_difference 8186..8192 /label=U4\deletion /note="U4 was not included in alpha" misc_difference 8287..8292 /label=extra\site misc_difference 1507..1513 /label=extra\site CDS 1345..1499 /label=gene0.4 misc_feature 1452..1457 /label=U1 misc_difference 3322..3327 /label=extra\site CDS 3344..5995 /label=gene1 misc_difference 7853..7854 /label=T\missing\inbetween misc_binding 825..832 /label=boxA misc_difference 1424..1425 /label=deleted\C\inbetween CDS 944..1297 /label=gene\0.3 CDS 1586..1729 /label=gene\0.5 CDS 1757..2094 /label=gene\0.6B CDS 1757..1918 /label=gene\0.6A CDS 2128..3207 /label=gene\0.7 CDS 6196..6324 /label=gene\1.1 CDS 6353..6610 /label=gene\1.2 CDS 6740..7819 /label=gene\1.3 CDS 7904..8059 /label=gene\1.4 CDS 8141..8230 /label=gene\1.5 CDS 8311..8571 /label=gene\1.6 CDS 8600..8750 /label=gene\1.7\fragment stem_loop 1514..1565 /label=RSL_R0.5 stem_loop 3258..3304 /label=RSL_R1 stem_loop 6036..6088 /label=RSL_R1.1 stem_loop 6652..6708 /label=RSL_R1.3 terminator 7842..7866 /label=TE stem_loop 7845..7863 /label=TSL_TE promoter 3221..3283 /label=C promoter 5828..5889 /label=S1 promoter 6010..6044 /label=phi1.1A promoter 6085..6119 /label=phi1.1.B promoter 6629..6663 /label=phi1.3 -10_signal 3257..3262 /label=-10_C -10_signal 5863..5868 /label=-10_S -35_signal 3234..3238 /label=-35_C -35_signal 5841..5845 /label=-35_S1 misc_structure 1555..1555 /label=C_R0.5 misc_structure 3225..3225 /label=-45_C misc_structure 3269..3269 /label=S_C misc_structure 3295..3295 /label=C_R1 misc_structure 5832..5832 /label=-45_S1 misc_structure 5875..5875 /label=S_S1 misc_structure 6037..6037 /label=S_phi1.1A misc_structure 6077..6077 /label=C_R1.1 misc_structure 6112..6112 /label=S_phi1.1B misc_structure 6656..6656 /label=S_phi1.3 misc_structure 7870..7870 /label=T_TE misc_structure 8097..8097 /label=S_phi1.5 misc_structure 8269..8269 /label=S_phi1.6 misc_difference 4897..4897 /label=C->T\mutation /note="silent" misc_difference 5179..5179 /label=C->T\mutation /note="silent" misc_difference 5419..5419 /label=G->A\mutation /note="silent" misc_difference 2018..2018 /label=difference /note="single base insertion difference between Molineux's T7 stock and the sequence of T7 published by Studier" BASE COUNT 2467 a 2024 c 2206 g 2053 t ORIGIN 1 tctcacagtg tacggaccta aagttccccc atagggggta cctaaagccc agccaatcac 61 ctaaagtcaa ccttcggttg accttgaggg ttccctaagg gttggggatg acccttgggt 121 ttgtctttgg gtgttacctt gagtgtctct ctgtgtccct atctgttaca gtctcctaaa 181 gtatcctcct aaagtcacct cctaacgtcc atcctaaagc caacacctaa agcctacacc 241 taaagaccca tcaagtcaac gcctatctta aagtttaaac ataaagacca gacctaaaga 301 ccagacctaa agacactaca taaagaccag acctaaagac gcccgcatgc ttgttgttag 361 ccataaagtg ataaccttta atcattgtct ttattaatac aactcactat aaggagagac 421 aacttaaaga gcatgcttaa aagattaatc gattaaaatt tatcaaaaag agtattgact 481 taaagtctaa cctataggat acttacagcc atcgagaggg acacggcgaa tagccatccc 541 aatcgatacc ggggtcaacc ggataagtag aaagcttgat aagtcgcacg aaaaacaggt 601 attgacaaca tgaagtaaca tgcagtaaga tacaaatcgc taggtaacac tagcagcgtc 661 aaccgggcgc acagtgcctt ctaggtgact taagcgcacc acggcacata aggtgaaaca 721 aaacggttga caacatgaag taaacacggt acgatgtacc acatgaaacg acagtgagtc 781 accacactga aaggtgatgc ggtctaacga aacctgacct aagacgctct ttaacaatct 841 ggtaaatagc tcttgagtgc atgactagcg gataactcaa gggtatcgca aggtgccctt 901 tatgatattc actaagcttg cgcgctgcac gaggtaacac aagatggcta tgtctaacat 961 gacttacaac aacgttttcg accacgctta cgaaatgctg aaagaaaaca tccgttatga 1021 tgacatccgt gacactgatg acctgcacga tgctattcac atggctgccg ataatgcagt 1081 tccgcactac tacgctgaca tctttagcgt aatggcaagt gagggcattg accttgagtt 1141 cgaagactct ggtctgatgc ctgacaccaa ggacgtaatc cgcatcctgc aagcgcgtat 1201 ctatgagcaa ttaacgattg acctctggga agacgcagaa gacttgctca atgaatactt 1261 ggaggaagtc gaggagtacg aggaggacga agagtaagcg cgcaccaggt accaggtcga 1321 ggagtacgag gaggatgaag agtaatgtct actaccaacg tgcaatacgg tctgaccgct 1381 caaactgtac ttttctatag cgacatggtg cgctgtggct ttaatggtca ctcgcaatgg 1441 cacagctgaa ggagctctac gaaaacaaca aggcaatagc tttagaatct gctgagtgaa 1501 ccaggtacca ggtgagtgat agactcaagg tcgctcctag cgagtggcct ttatgattat 1561 cacttacgcg tcttatgagg gagtaatgta tatgcttact atcggtctac tcaccgctct 1621 agggctagct gtaggtgcat cctttgggaa ggctttaggt gtagctgtag gttcctactt 1681 taccgcttgc atcatcatag gaatcatcaa aggcgcacta cgcaaataaa cgcgtacgca 1741 aaggggcact acgcaaatga tgaagcacta cgttatgcca atccacacgt ccaacggggc 1801 aaccgtatgt acacctgatg ggttcgcaat gaaacaacga atcgaacgcc ttaagcgtga 1861 actccgcatt aaccgcaaga ttaacaagat aggttccggc tatgacagaa cgcactgatg 1921 gcttaaagaa aggttatatg cccaatggca cactatacgc tgcaaatcgg cgaatagtga 1981 gaacttggcg agagaacaac ctcgaacgcc gcaaggaaca agagagggcg gcgtggcata 2041 gacgaaagga aaaggttaaa gccaagaaac tcgccgcact tgaacaggca ctagcgtacg 2101 gtccgcgaac ataaaggaca caatgcaatg aacattaccg acatcatgaa cgctatcgac 2161 gcaatcaaag cactgccaat ctgtgaactt gacaagcgtc aaggtatgct tatcgactta 2221 ctggtcgaga tggtcaacag cgagacgtgt gatggcgagc taaccgaact aaatcaggca 2281 cttgagcatc aagattggtg gactaccttg aagtgtctca cggctgacgc agggttcaag 2341 atgctcggta atggtcactt ctcggctgct tatagtcacc cgctgctacc taacagagtg 2401 attaaggtgg gctttaagaa agaggattca ggcgcagcct ataccgcatt ctgccgcatg 2461 tatcagggtc gtcctggtat ccctaacgtc tacgatgtac agcgccacgc tggatgctat 2521 acggtggtac ttgacgcact taaggattgc gagcgtttca acaatgatgc ccattataaa 2581 tacgctgaga ttgcaagcga catcattgat tgcaattcgg atgagcatga tgagttaact 2641 ggatgggatg gtgagtttgt tgaaacttgt aaactaatcc gcaagttctt tgagggcatc 2701 gcctcattcg acatgcatag cgggaacatc atgttctcaa atggagacgt accatacatc 2761 accgacccgg tatcattctc gcagaagaaa gacggtggcg cattcagcat cgaccctgag 2821 gaactcatca aggaagtcga ggaagtcgca cgacagaaag aaattgaccg cgctaaggcc 2881 cgtaaagaac gtcacgaggg gcgcttagag gcacgcagat tcaaacgtcg caaccgcaag 2941 gcacgtaaag cacacaaagc taagcgcgaa agaatgcttg ctgcgtggcg atgggctgaa 3001 cgtcaagaac ggcgtaacca tgaggtagct gtagatgtac taggaagaac caataacgct 3061 atgctctggg tcaacatgtt ctctggggac tttaaggcgc ttgaggaacg aatcgcgctg 3121 cactggcgta atgctgaccg gatggctatc gctaatggtc ttacgctcaa cattgataag 3181 caacttgacg caatgttaat gggctgacgg tccgccgcgg attgataagc aacttgacgc 3241 aatgttaatg ggctgatagt cttatcttac aggtcatctg cgggtggcct gaataggtac 3301 gatttactaa cccgcggccg acggccgctg gaagaggcac taaatgaaca cgattaacat 3361 cgctaagaac gacttctctg acatcgaact ggctgctatc ccgttcaaca ctctggctga 3421 ccattacggt gagcgtttag ctcgcgaaca gttggccctt gagcatgagt cttacgagat 3481 gggtgaagca cgcttccgca agatgtttga gcgtcaactt aaagctggtg aggttgcgga 3541 taacgctgcc gccaagcctc tcatcactac cctactccct aagatgattg cacgcatcaa 3601 cgactggttt gaggaagtga aagctaagcg cggcaagcgc ccgacagcct tccagttcct 3661 gcaagaaatc aagccggaag ccgtagcgta catcaccatt aagaccactc tggcttgcct 3721 aaccagtgct gacaatacaa ccgttcaggc tgtagcaagc gcaatcggtc gggccattga 3781 ggacgaggct cgcttcggtc gtatccgtga ccttgaagct aagcacttca agaaaaacgt 3841 tgaggaacaa ctcaacaagc gcgtagggca cgtctacaag aaagcattta tgcaagttgt 3901 cgaggctgac atgctctcta agggtctact cggtggcgag gcgtggtctt cgtggcataa 3961 ggaagactct attcatgtag gagtacgctg catcgagatg ctcattgagt caaccggaat 4021 ggttagctta caccgccaaa atgctggcgt agtaggtcaa gactctgaga ctatcgaact 4081 cgcacctgaa tacgctgagg ctatcgcaac ccgtgcaggt gcgctggctg gcatctctcc 4141 gatgttccaa ccttgcgtag ttcctcctaa gccgtggact ggcattactg gtggtggcta 4201 ttgggctaac ggtcgtcgtc ctctggcgct ggtgcgtact cacagtaaga aagcactgat 4261 gcgctacgaa gacgtttaca tgcctgaggt gtacaaagcg attaacattg cgcaaaacac 4321 cgcatggaaa atcaacaaga aagtcctagc ggtcgccaac gtaatcacca agtggaagca 4381 ttgtccggtc gaggacatcc ctgcgattga gcgtgaagaa ctcccgatga aaccggaaga 4441 catcgacatg aatcctgagg ctctcaccgc gtggaaacgt gctgccgctg ctgtgtaccg 4501 caaggacaag gctcgcaagt ctcgccgtat cagccttgag ttcatgcttg agcaagccaa 4561 taagtttgct aaccataagg ccatctggtt cccttacaac atggactggc gcggtcgtgt 4621 ttacgctgtg tcaatgttca acccgcaagg taacgatatg accaaaggac tgcttacgct 4681 ggcgaaaggt aaaccaatcg gtaaggaagg ttactactgg ctgaaaatcc acggtgcaaa 4741 ctgtgcgggt gtcgataagg ttccgttccc tgagcgcatc aagttcattg aggaaaacca 4801 cgagaacatc atggcttgcg ctaagtctcc actggagaac acttggtggg ctgagcaaga 4861 ttctccgttc tgcttccttg cgttctgctt tgagtatgct ggggtacagc accacggcct 4921 gagctataac tgctcccttc cgctggcgtt tgacgggtct tgctctggca tccagcactt 4981 ctccgcgatg ctccgagatg aggtaggtgg tcgcgcggtt aacttgcttc ctagtgaaac 5041 cgttcaggac atctacggga ttgttgctaa gaaagtcaac gagattctac aagcagacgc 5101 aatcaatggg accgataacg aagtagttac cgtgaccgat gagaacactg gtgaaatctc 5161 tgagaaagtc aagctgggta ctaaggcact ggctggtcaa tggctggctt acggtgttac 5221 tcgcagtgtg actaagcgtt cagtcatgac gctggcttac gggtccaaag agttcggctt 5281 ccgtcaacaa gtgctggaag ataccattca gccagctatt gattccggca agggtctgat 5341 gttcactcag ccgaatcagg ctgctggata catggctaag ctgatttggg aatctgtgag 5401 cgtgacggtg gtagctgcag ttgaagcaat gaactggctt aagtctgctg ctaagctgct 5461 ggctgctgag gtcaaagata agaagactgg agagattctt cgcaagcgtt gcgctgtgca 5521 ttgggtaact cctgatggtt tccctgtgtg gcaggaatac aagaagccta ttcagacgcg 5581 cttgaacctg atgttcctcg gtcagttccg cttacagcct accattaaca ccaacaaaga 5641 tagcgagatt gatgcacaca aacaggagtc tggtatcgct cctaactttg tacacagcca 5701 agacggtagc caccttcgta agactgtagt gtgggcacac gagaagtacg gaatcgaatc 5761 ttttgcactg attcacgact ccttcggtac cattccggct gacgctgcga acctgttcaa 5821 agcagtgcgc gaaactatgg ttgacacata tgagtcttgt gatgtactgg ctgatttcta 5881 cgaccagttc gctgaccagt tgcacgagtc tcaattggac aaaatgccag cacttccggc 5941 taaaggtaac ttgaacctcc gtgacatctt agagtcggac ttcgcgttcg cgtaacggcc 6001 gttaattaaa acgccaaatc aatacgactc actatagagg gacaaactca aggtcattcg 6061 caagagtggc ctttatgatt gaccttcttc cggttaatac gactcactat aggagaacct 6121 taaggtttaa ctttaagacc cttaagtgtt aattaaagat ttaaattaaa gaattcctaa 6181 gagaggactt taagtatgcg taacttcgaa aagatgacca aacgttctaa ccgtaatgct 6241 cgtgacttcg aggcaaccaa aggtcgcaag ttgaataaga ctaagcgtga ccgctctcac 6301 aagcgtagct gggagggtca gtaagaattc caggactggg agggtcagta agatgggacg 6361 tttatatagt ggtaatctgg cagcattcaa ggcagcaaca aacaagctgt tccagttaga 6421 cttagcggtc atttatgatg actggtatga tgcctataca agaaaagatt gcatacggtt 6481 acgtattgag gacaggagtg gaaacctgat tgatactagc accttctacc accacgacga 6541 ggacgttctg ttcaatatgt gcactgattg gttgaaccat atgtatgacc agttgaagga 6601 ctggaagtaa tccaggaatc caggacccgg actggaagta atacgactca gtatagggac 6661 aatgcttaag gtcgctctct aggagtggcc ttagtcattt aaccaatagg aggacccggg 6721 ccaataggag ataaacatta tgatgaacat taagactaac ccgtttaaag ccgtgtcttt 6781 cgtagagtct gccattaaga aggctctgga taacgctggg tatcttatcg ctgaaatcaa 6841 gtacgatggt gtacgcggga acatctgcgt agacaatact gctaacagtt actggctctc 6901 tcgtgtatct aaaacgattc cggcactgga gcacttaaac gggtttgatg ttcgctggaa 6961 gcgtctactg aacgatgacc gttgcttcta caaagatggc tttatgcttg atggggaact 7021 catggtcaag ggcgtagact ttaacacagg gtccggccta ctgcgtacca aatggactga 7081 cacgaagaac caagagttcc atgaagagtt attcgttgaa ccaatccgta agaaagataa 7141 agttcccttt aagctgcaca ctggacacct tcacataaaa ctgtacgcta tcctcccgct 7201 gcacatcgtg gagtctggag aagactgtga tgtcatgacg ttgctcatgc aggaacacgt 7261 taagaacatg ctgcctctgc tacaggaata cttccctgaa atcgaatggc aagcggctga 7321 atcttacgag gtctacgata tggtagaact acagcaactg tacgagcaga agcgagcaga 7381 aggccatgag ggtctcattg tgaaagaccc gatgtgtatc tataagcgcg gtaagaaatc 7441 tggctggtgg aaaatgaaac ctgagaacga agctgacggt atcattcagg gtctggtatg 7501 gggtacaaaa ggtctggcta atgaaggtaa agtgattggt tttgaggtgc ttcttgagag 7561 tggtcgttta gttaacgcca cgaatatctc tcgcgcctta atggatgagt tcactgagac 7621 agtaaaagag gccaccctaa gtcaatgggg attctttagc ccatacggta ttggcgacaa 7681 cgatgcttgt actattaacc cttacgatgg ctgggcgtgt caaattagct acatggagga 7741 aacacctgat ggctctttgc ggcacccatc gttcgtaatg ttccgtggca ccgaggacaa 7801 ccctcaagag aaaatgtaac ccgggcccaa aatgtaatca cactggctca cctcgggtgg 7861 gcctttcagg gaaaacgggc ccatggttat aaggagacac tttatgttta agaaggttgg 7921 taaattcctt gcggctttgg cagctatcct gacgcttgcg tatattcttg cggtataccc 7981 tcaagtagca ctagtagtag ttggcgcttg ttacttagcg gcagtgtgtg cttgcgtgtg 8041 gagtatagtt aactggtaat ccatggcgcc gttaactggt aatacgactc actaaaggag 8101 gtacggcgcc taggcctagg cactaaagga ggtacacacc atgatgtact taatgccatt 8161 actcatcgtc attgtaggat gccttgcgct ccactgtagc gatgatgata tgccagatgg 8221 tcacgcttaa cctaggacgt ctggtcacgc ttaatacgac tcactaaagg agacacgacg 8281 tctagatcta gacactaaag gagacactat atgtttcgac ttcattacaa caaaagcgtt 8341 aagaatttca cggttcgccg tgctgaccgt tcaatcgtat gtgcgagcga gcgccgagct 8401 aagatacctc ttattggtaa cacagttcct ttggcaccga gcgtccacat cattatcacc 8461 cgtggtgact ttgagaaagc aatagacaag aaacgtccgg ttcttagtgt ggcagtgacc 8521 cgcttcccgt tcgtccgtct gttactcaaa cgaatcaaag aagtgttctg atctagaacc 8581 ggttcaagga ggtgttctga tgggactgtt agatggtgaa gcctgggaaa aagaaaaccc 8641 gccagtacaa gcaactgggt gtatagcttg cttagagaaa gatgaccgtt atccacacac 8701 ctgtaacaaa ggagctaacg atatgaccga acgtgaacaa gagatgatca //