Publications

Publication Highlights

Spontaneous levels of base excision repair shown to pressure homologous recombination in vivo, Kiraly O, Gong G, Roytman MD, Yamada Y, Samson LD, Engelward BP. DNA glycosylase activity and cell proliferation are key factors in modulating homologous recombination in vivo. Carcinogenesis. 2014 Aug 25. pii: bgu177.

Nanoparticles shown to be genotoxic, Watson C, Ge J, Cohen J, Pyrgiotakis G, Engelward BP, Demokritou P. High-throughput screening platform for engineered nanoparticle-mediated genotoxicity using CometChip technology. ACS Nano. 2014 Mar 25;8(3):2118-33. In the MIT News.

Second generation mouse model enables studies of homologous recombination in a broad range of tissue types and reveals accumulation of recombination events in somatic stem cells. Sukup-Jackson MR, Kiraly O, Kay JE, Na L, Rowland EA, Winther KE, Chow DN, Kimoto T, Matsuguchi T, Jonnalagadda VS, Maklakova VI, Singh VR, Wadduwage DN, Rajapakse J, So PT, Collier LS, Engelward BP. Rosa26-GFP direct repeat (RaDR-GFP) mice reveal tissue- and age-dependence of homologous recombination in mammals in vivo. PLoS Genet. 2014 Jun5;10(6):e1004299. In the news.

CometChip enables high throughput screening of DNA double strand breaks. Weingeist DM, Ge J, Wood DK, Mutamba JT, Huang Q, Rowland EA, Yaffe MB, Floyd S, and Engelward BP. Single cell microarray enables high throughput evaluation of DNA double strand breaks and DNA repair inhibitors. Cell Cycle. 2013 Mar 15;12(6):907-15.
News and Views by Nickoloff J.A., Assaying DNA double-strand break induction and repair as fast as a speeding comet, Cell Cycle. 2013 12:1335-6.

Imaging approaches for suppression of light-induced DNA damage. Ge J, Wood DK, Weingeist DM, Prasongtanakig S, Navasumrit P, Ruchirawat M, Engelward BP. Standard fluorescent imaging of live cells is highly genotoxic. Cytometry A. 2013 Jun;83(6):552-60. Editorial by Tárnok A., Visualization can be harmful for live cells, Cytometry A., 2013 83:521-2.

MIT study suggests that at low dose-rate, radiation poses little risk to DNA. In the news and on the MIT Homepage! (also featured by MIT as "Best of 2012") News of this study was dispersed to over 50 sites worldwide. Olipitz W, Wiktor-Brown D, Shuga J, Pang B, McFaline J, Lonkar P, Thomas A, Mutamba JT, Greenberger JS, Samson LD, Dedon PC, Yanch JC, Engelward BP. Integrated molecular analysis indicates undetectable change in DNA damage in mice after continuous irradiation at ~ 400-fold natural background radiation. Environ Health Perspect. 2012 Aug;120(8):1130-6.

Novel technology for high throughput analysis of DNA damage In the news and on the MIT Homepage!
Wood DK, Weingeist DM, Bhatia SN, Engelward BP. Single cell trapping and DNA damage analysis using microwell arrays. Proc Natl Acad Sci U S A. 2010 Jun 1;107(22):10008-13.

Burden of mutant cells is driven primarily by cell division in the pancreas In the news
Wiktor-Brown DM, Kwon HS, Nam YS, So PT, Engelward BP. Integrated one- and two-photon imaging platform reveals clonal expansion as a major driver of mutation load. Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10314-9.

Recombination mechanisms come to life with animations See Animations
Top 10 downloaded papers in DNA Repair for 2008-2011; Most highly cited article in 2012
Helleday T, Lo J, van Gent DC, Engelward BP. DNA double-strand break repair: from mechanistic understanding to cancer treatment. DNA Repair (Amst). 2007 Jul 1;6(7):923-35.

Visualization of fluorescent recombinant cells reveals accumulation with age
Wiktor-Brown DM, Hendricks CA, Olipitz W, Engelward BP. Age-dependent accumulation of recombinant cells in the mouse pancreas revealed by in situ fluorescence imaging. Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11862-7.

Indirect induction of homologous recombination is transmissible
Rugo RE, Almeida KH, Hendricks CA, Jonnalagadda VS, Engelward BP. A single acute exposure to a chemotherapeutic agent induces hyper-recombination in distantly descendant cells and in their neighbors. Oncogene. 2005 Jul 28;24(32):5016-25.

Chemical mechanisms of inflammation-induced sequence rearrangements
Kiziltepe T, Yan A, Dong M, Jonnalagadda VS, Dedon PC, Engelward BP. Delineation of the chemical pathways underlying nitric oxide-induced homologous recombination in mammalian cells. Chem Biol. 2005 Mar;12(3):357-69.

Fluorescence detection of recombination in vivo
Hendricks CA, Almeida, KH, Stitt, MS, Jonnalagadda, VS, Rugo, RE, Kerrison, GF, Engelward BP. Spontaneous mitotic homologous recombination at an enhanced yellow fluorescent protein (EYFP) cDNA direct repeat in transgenic mice. Proc. Natl. Acad. Sci. USA. 2003 May 27;100(11):6325-30.

DNA repair enzymes can promote nitric-oxide-induced recombination
Spek EJ, Vuong LN, Matsuguchi T, Marinus MG, Engelward BP. Nitric oxide-induced homologous recombination in Escherichia coli is promoted by DNA glycosylases. J. Bacteriol. 2002 Jul;184(13): 3501-7.


 

Selected Publications

Kiraly O, Gong G, Roytman MD, Yamada Y, Samson LD, Engelward BP. DNA glycosylase activity and cell proliferation are key factors in modulating homologous recombination in vivo. Carcinogenesis. 2014 Aug 25. pii: bgu177.

Sukup-Jackson MR, Kiraly O, Kay JE, Na L, Rowland EA, Winther KE, Chow DN, Kimoto T, Matsuguchi T, Jonnalagadda VS, Maklakova VI, Singh VR, Wadduwage DN, Rajapakse J, So PT, Collier LS, Engelward BP. Rosa26-GFP direct repeat (RaDR-GFP) mice reveal tissue- and age-dependence of homologous recombination in mammals in vivo. PLoS Genet. 2014 Jun5;10(6):e1004299.

Watson C, Ge J, Cohen J, Pyrgiotakis G, Engelward BP, Demokritou P. High-throughput screening platform for engineered nanoparticle-mediated genotoxicity using CometChip technology. ACS Nano. 2014 Mar 25;8(3):2118-33.

Kumar Y, Liang C, Limmon GV, Liang L, Engelward BP, Ooi EE, Chen J, Tannenbaum SR. Molecular analysis of serum and bronchoalveolar lavage in a mouse model of influenza reveals markers of disease severity that can be clinically useful in humans. PLoS One. 2014 Feb 5;9(2):e86912.

Ge J, Wood DK, Weingeist DM, Prasongtanakig S, Navasumrit P, Ruchirawat M, Engelward BP. Standard fluorescent imaging of live cells is highly genotoxic. Cytometry A. 2013 Jun;83(6):552-60.

Wilson TE, DeMarini DM, Dertinger SD, Engelward BP, Hanawalt PC, MacGregor JT, Smith-Roe SL, Witt KL, Yauk CL, Ljungman M, Schwartz JL, Klein CB. Building on the past, shaping the future: the Environmental Mutagenesis and Genomics Society. Environ Mol Mutagen. 2013 Apr;54(3):153-7.

Weingeist DM, Ge J, Wood DK, Mutamba JT, Huang Q, Rowland EA, Yaffe MB, Floyd S, and Engelward BP. Single cell microarray enables high throughput evaluation of DNA double strand breaks and DNA repair inhibitors. Cell Cycle. 2013 Mar 15;12(6):907-15.

Li N, Yin L, Thévenin D, Yoshiyuki Y, Limmon G, Chen J, Chow VT, Engelman DM, Engelward BP. Peptide targeting and imaging of damaged lung tissue in influenza-infected mice. Future Microbiol. 2013 Feb;8(2):257-69. 
  
Ivan FX, Tan KS, Phoon MC, Engelward BP, Welsch RE, Rajapakse JC, Chow VT. Neutrophils infected with highly virulent influenza H3N2 virus exhibit augmented early cell death and rapid induction of type I interferon signaling pathways. Genomics. 2013 101:101-112.  
                          
Olipitz W, Wiktor-Brown D, Shuga J, Pang B, McFaline J, Lonkar P, Thomas A, Mutamba JT, Greenberger JS, Samson LD, Dedon PC, Yanch JC, Engelward BP. Integrated molecular analysis indicates undetectable change in DNA damage in mice after continuous irradiation at ~ 400-fold natural background radiation. Environ Health Perspect. 2012 Aug;120(8):1130-6.

Yamada Y, Limmon GV, Zheng D, Li N, Li L, Yin L, Chow VT, Chen J, Engelward BP. Major shifts in the spatio-temporal distribution of lung antioxidant enzymes during influenza pneumonia. PLoS One. 2012;7(2):e31494.

Mutamba JT, Svilar D, Prasongtanakii S, Wang XH, Lin YC, Dedon PC, Sobol RW, Engelward BP. XRCC1 and base excision repair balance in response to nitric oxide. DNA Repair (Amst). 2011 Dec 10;10(12):1282-93.
 
Wiktor-Brown DM, Sukup-Jackson MR, Fakhraldeen SA, Hendricks CA, Engelward BP. p53 null fluorescent yellow direct repeat (FYDR) mice have normal levels of homologous recombination. DNA Repair (Amst). 2011 Dec 10;10(12):1294-9.

Rugo RE, Mutamba JT, Mohan KN, Yee T, Chaillet JR, Greenberger JS, and Engelward BP. Methyltransferases mediate cell memory of a genotoxic insult. Oncogene. 2011 Feb 10;30(6):751-756.

Wood DK, Weingeist DM, Bhatia SN, Engelward BP. Single cell trapping and DNA damage analysis using microwell arrays. Proc Natl Acad Sci U S A. 2010 Jun 1;107(22):10008-13.

Olipitz W, Hembrador S, Davidson M, Yanch JC, Engelward BP. Development and characterization of a novel variable low dose-rate irradiator for in vivo mouse studies. Health Phys. 2010 May;98(5):727-34.

Wiktor-Brown DM, Kwon HS, Nam YS, So PT, Engelward BP. Integrated one- and two-photon imaging platform reveals clonal expansion as a major driver of mutation load. Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10314-9.

Wiktor-Brown DM, Olipitz W, Hendricks CA, Rugo RE, Engelward BP. Tissue-specific differences in the accumulation of sequence rearrangements with age. DNA Repair (Amst). 2008 May 3;7(5):694-703.

Helleday T, Lo J, van Gent DC, Engelward BP. DNA double-strand break repair: from mechanistic understanding to cancer treatment. DNA Repair (Amst). 2007 Jul 1;6(7):923-35.

Wiktor-Brown DM, Hendricks CA, Olipitz W, Engelward BP. Age-dependent accumulation of recombinant cells in the mouse pancreas revealed by in situ fluorescence imaging. Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11862-7.

Nowosielska A, Smith SA, Engelward BP, Marinus MG. Homologous recombination prevents methylation-induced toxicity in Escherichia coli. Nucleic Acids Res. 2006 May 2;34(8):2258-68.

Rugo RE, Almeida KH, Hendricks CA, Jonnalagadda VS, Engelward BP. A single acute exposure to a chemotherapeutic agent induces hyper-recombination in distantly descendant cells and in their neighbors. Oncogene. 2005 Jul 28;24(32):5016-25.

Jonnalagadda VS, Matsuguchi T, Engelward BP. Interstrand crosslink-induced homologous recombination carries an increased risk of deletions and insertions. DNA Repair (Amst). 2005 May 2;4(5):594-605.

Kiziltepe T, Yan A, Dong M, Jonnalagadda VS, Dedon PC, Engelward BP. Delineation of the chemical pathways underlying nitric oxide-induced homologous recombination in mammalian cells. Chem Biol. 2005 Mar;12(3):357-69.