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HepB and HIV Infection

Inactivation of Hepatitis B Virus

Agent Concentration Exposure Time Temperature (C) Reference Number
Sodium Hypochlorite(1:10 dilution of bleach) 0.525% 10 min. 20 1
Isopropyl Alcohol 70% 10 min. 20 1
Iodophor(1:213 dilution of Wescodyne 10 min. 20 1
Ethanol 80% 2 min. 11 2
Heat 2 min. 98 2


Inactivation of HIV Virus

Agent Concentration Exposure Time Temperature (C) Reference Number
H(2)O(2) 0.3% 2-10 min. 21-25 3
Ethyl Alcohol 50% 2-10 min. 21-25 3,6
Isopropyl Alcohol 35.0% 2-10 min. 21-25 3
Bleach 0.1% 2-10 min. 21-25 3
10% 1 min. room 9
Lysol 0.5% 2-10 min. 21-25 3
Quaternary Ammonium 0.08% 10 min. room 9
NaOH 40.0 mml 5 min. 8
13<=pH<=1 unknown 21-25 3
Heat 10 min. 56 3
30 min. 56 5,7,10
Heat(in media with 50% human plasma) 5 hrs 54-56 9
Heat (wet heat) 10 hrs 60 10
Heat(virus in lyophilized culture) 48 hrs 68 10
Heat(virus in lyophilized Autoplex) 144 hrs 60 11
11-15 days 36-37 9*
Desiccation (in media) 3-7 days 23-27 9
Desiccation (in cell associated state) 1-3 days 30 9
Povidone-lodine 0.19% 1 min. 20 13
0.25%% <1 min. 20 13

* Virus survived in media with 50% human plasma at 23-27 degrees C for the entire test period, 15 days.

A pH reduction from 7 to 6 reseults in about a 3 log reduction in HIV titer (3).

On the open bench, HIV in media with 50% human plasma, there was a 6 log reduction in 72 hours with 1 log further reduction in an additional 24 hours (9). HIV at 54-56 degrees C in media with 50% human plasma had a 3 log reduction in the first hour, and 6 logs after 3 hours (9).





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