Single-Particle Electron Microscopy
Dr. James Chen
Tue Jan 18, Thu Jan 20, Tue Jan 25, Thu Jan 27, 02-03:00pm, 68-181
No enrollment limit, no advance sign up
Recent advances in single-particle electron microscopy (EM), and cryo-EM in particular, have enabled structural analysis of macromolecular complexes at sub-nanometer and even near-atomic resolution. Complementary to X-ray crystallography and NMR spectroscopy, single-particle EM is unique in its capability to elucidate the structures of large molecular assemblies in their native states. In this seminar series, I will introduce the theoretical foundation as well as the basic experimental techniques of single-particle EM. The topics will include: the electron microscope instrument, sample preparation, data acquisition, image analysis, model reconstruction, and software hands-on practice. This course will emphasize on basic principles and concept (rather than on math and algorithms), and is open to the entire MIT biology research community (undergraduates, graduates, postdocs and faculty members).
PLEASE NOTE: The January 27 class will meet in Room 68-474.
Contact: Dr. James Chen, 68-480, x3-8688, jzchen@mit.edu
Sponsor: Biology
Latest update: 28-Dec-2010
|
|
