MIT: Independent Activities Period: IAP

IAP 2017

Introduction to sterile technique on the bench and biosafety cabinet

Lorena Altamirano, Biosafety Officer, EHS

Enrollment: Limited: Advance sign-up required
Sign-up by 01/06
Limited to 10 participants
Attendance: Participants must attend all sessions

Sterile or aseptic technique forms the basis of microbiology and tissue culturing method to prevent contamination of samples and safeguard people. This course is intended as an introduction to the theory and practice of sterile technique. This course would focus on sterile technique at the bench (basic bacteriology) as well as using a biological safety cabinet (BSC) for basic cell/tissue culturing.

Target audience: The course is intended for people from non-traditional biological sciences backgrounds such as engineering, physics, arts, architecture, etc.

Prereq: None; no biological experience is needed.


Sponsor(s): Environment, Health and Safety Office, Biology
Contact: Lorena Altamirano, 617-253-3844,


Jan/10 Tue 10:00AM-12:00PM N52-496, Conference Room A

Lecture on principles and practices, equipment basics, materials and methods and protocol review.

Lorena Altamirano - Biosafety Officer, EHS

Laboratory Practice

Jan/11 Wed 10:00AM-12:00PM 68-088
Jan/12 Thu 10:00AM-12:00PM 68-082
Jan/13 Fri 10:00AM-12:00PM 68-088

Laboratory practices, equipment, protocol application and biosafety. Discussion and troubleshooting.

Must attend all lab pratice dates.

Lorena Altamirano - Biosafety Officer, EHS