2017 “Targeting Bacillosamine Biosynthesis in Bacterial Pathogens: Development of Inhibitors to a Bacterial Amino-Sugar Acetyl Transferase from Campylobacter jejuni”, De Schutter, J. W.; Morrison, J. P.; Morrison, M. J.; Ciulli, A.; Imperiali, B. J. Med. Chem 2017, 60(5), 2099-2118. PMC5506375 “Conformational Dynamics and Alignment Properties of Loop Lanthanide-Binding Tags (LBTs) Studied in Interleukin-1β”, Schwalbe, H.; Barthelmes, D.; Barthelmes, K.; Schnorr, K.; Hendrik R. A.; Jonker B. B.; Allen, K. N.; Imperiali, B. J. Biol NMR, 2017, May 22. DOI:10.1007/s10858-017-0118-5. “Analysis of a Dual Domain Phosphoglycosyl Transferase Reveals a Ping-Pong Mechanism with a Covalent Enzyme Intermediate”, Das, D.; Suzmic, P.; Imperiali, B. Proc. Natl. Acad. Sci. USA 2017, 114(27): 7019-7024. PMC5502628 “Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars”, Zamora, C.; Schocker, N. S.; Chang, M. M.; Imperiali, B. Meth. Enzymol. 2017, in press. “Bacterial Phosphoglycosyl Transferases: Initiators of Glycan Biosynthesis at the Membrane Interface”, Lukose, V.; Walvoort, M. T. C.; Imperiali, B. Glycobiology, 2017, in press. 2016 “A Modular Approach to Phosphoglycosyl Transferase Inhibitors Inspired by Nulceoside Antibiotics” Walvoort, M. T. C.; Lukose, V.; Imperiali, B. Chem. Eur. J. 2016, 22, 3856-3864. PMC5506376 “Probing Polytopic Membrane Protein - Substrate Interactions by Luminescence Resonance Energy Transfer” Musial-Siwek, M.; Jafee, M. B.; Imperiali, B. J. Am. Chem. Soc. 2016, 3806-3812. PMC5480290 “Design Principles for SuCESsFul Biosensors: Specific Fluorophore/Analyte Binding and Minimization of Fluorophore/Scaffold Interactions”. de Picciotto, S.; Dickson, P. M.; Traxlmayr, M. W.; Marques, B. S.; Socher, E.; Zhao, S.; Cheung, S.; Kiefer, J.; Griffith, L. G.; Wand, A. J.; Imperiali, B.; Wittrup, K. D. J. Mol. Biol. 2016, 428, 2228-2241. PMC5048519 DOI: 10.1016/j.jmb.2016.07.004 “A Rapid and Efficient Luminescence-based Method for Assaying Phosphoglycosyl-transferase Enzymes” Das, D.; Walvoort, M. T. C.; Lukose, V.; Imperiali, B. Scientific Reports, 2016, 6:33412. PMC5022061. “Bacterial N-Glycosylation Efficiency is Dependent on the Structural Context of Target Sequons” Silverman, J. M.; Imperiali, B. J. Biol. Chem. 2016, 291, 22001-22010. PMC5063983 doi:10.1074/jbc.M116.747121. “Antibodies from Multiple Sclerosis Patients Preferentially Recognize Hyperglucosylated Adhesin of Non-Typeable Haemophilus influenzae“, Walvoort, M. T. C.; Testa, C.; Eilam, R.; Aharoni, R.; Nuti, F.; Rossi, G.; Real-Fernandez, R.; Lanzillo, R.; Morra, V. B.; Lolli, F.; Rovero, P.; Imperiali, B.; Papini, A-M. Scientific Reports, 2016, 6:39430. PMC5180199 . 2015 “The Best and the Brightest: Exploiting Tryptophan-Sensitized Tb3+ Luminescence to Engineer Lanthanide-Binding Tags” Martin, L. J.; Imperiali, B. Methods in Molecular Biology 2015, 1248, 201-220. ISBN 978-1-4939-2019-8. ”Selective Biochemical Labeling of C. jejuni Cell-Surface Glycoconjugates” Whitworth, G.; Imperiali, B. Glycobiology 2015, 25, 756-766. PMC25761366 ”N-Linked Glycans are Assembled on Highly Reduced Dolichol Phosphate Carriers in the Hyperthermophilic Archaea Pyrococcus furiosus,” Chang, M. M; Imperiali, B.; Eichler, J; Guan, Z. PLoS One 2015, 10 6 e0130482. PMC4476749 “Covalent Modification Of Synthetic Hydrogels With Bioactive Proteins Via Sortase-Mediated Ligation“ Cambria, E.; Renggli, K.; Ahrens, C. C.; Cook, C. D.; Kroll, C.; Krueger, A. T.; Imperiali, B.; Griffith, L. G. Biomacromolecules, 2015, 16, 2316-2326. PMC4613866 “Encoded Loop-Lanthanide-Binding Tags for Long-Range Distance Measurements in Proteins by NMR and EPR Spectroscopy” Barthelmes, D.; Granz, M.; Barthelmes, K.; Allen, K. N.; Imperiali, B.; Prisner, T.; and Schwalbe, H. J. Biomol. NMR. 2015, doi: 10.1007/s10858-015-9984-x. “Chemoenzymatic Assembly of Bacterial Glycoconjugates for Site-Specific Orthogonal Labeling” Lukose, V; Whitworth, G; Guan, Z.; Imperiali, B. J. Am. Chem. Soc. 2015, 137, 12446-12449. PMC4599313. “Conservation and Covariance in Monotopic Phosphoglycosyltransferases identifies the Functional Catalytic Core” Lukose, V.; Luo, L.; Kozakov, D.; Vajda, S.; Allen, K. N.; Imperiali, B. Biochemistry 2015, PMC5483379 2014 “The Renaissance of Bacillosamine and Its Derivatives: Pathway Characterization and Implications in Pathogenicity” Morrison, M. J. Imperiali, B. Biochemistry, 2014, 53, 624-638. PMC3951908 “Tailoring Chimeric Ligands for Studying and Biasing ErbB Receptor Family Interactions" Krueger, A. T.; Kroll, C.; Sanchez, E.; Griffith, L. G.; Imperiali, B. Angew. Chem. Int. Ed. 2014, 53, 2662-2666. PMC4018821 “Equilibrium and Dynamic Design Principles for Binding Molecules Engineered for Reagentless Biosensors” de Picciotto, S.; Imperiali, B.; Griffith, L. G.; Wittrup, K. D. Analytical Biochem. 2014, 460, 9-15. PMC4104283 “Selective Mitogen Activated Protein Kinase Activity Sensors through the Application of Directionally-Programmable D Domain Motifs” Peterson, L. B.; Yaffe, M. B.; Imperiali, B. Biochemistry 2014, 53, 5771-5778. PMC4165445 “Quantification of Protein Kinase Enzymatic Activity in Unfractionated Cell Lysates Using CSox-Based Sensors“ Beck, J. R.; Peterson, L. B.; Imperiali, B.; Stains, C. I. Curr. Protoc. Chem. Biol. 2014, 6, 135-156. PMC4174361 Peterson, L. B.; Imperiali, B. (2014) Real-Time and Continuous Sensors of Protein Kinase Activity Utilizing Chelation-Enhanced Fluorescence. Book Chapter in “Concepts and Case Studies in Chemical Biology”, (Edited by H. Waldmann and P. Janning) ISBN: 978-3-527-33611-1. 2013 "FRET-CAPTURE: A Sensitive Method for the Detection of Dynamic Protein Interactions” Socher, E.; Imperiali B. ChemBioChem 2013, 14, 53-57. PMC3776414 "Caged Mono- and Divalent Ligands for Light-Assisted Disruption of PDZ Domain-Mediated Interactions” Sainlos, M.; Iskenderian-Epps, W. S.; Olivier, N. B.; Choquet, D.; Imperiali B. J. Am. Chem. Soc. 2013, 135(12), 4580-4583. doi: 10.1021/ja309870q "Biochemical Evidence for an Alternate Pathway in N-linked Glycoprotein Biosynthesis" Larkin, A.; Chang, M. M.; Whitworth, G.E.; Imperiali, B. Nature Chemical Biology, 2013, 9, 367-373. PMC3661703 "Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology” Krueger, A. T.; Imperiali B. ChemBioChem, 2013, 14, 788-799. DOI: 10.1002/cbic.201300079 "Optimized Protocol for Expression and Purification of PglB the Membrane-Bound Bacterial Oligosaccharyl Transferase" Jaffee, M. B.; Imperiali, B. Protein Expression & Purification, 2013, 89, 241-250. PMC3690457 “Biosynthesis of UDP-N,N’Diacetylbacillosamine in Acinetobacter baumannii: Biochemical Characterization and Correlation to Existing Pathways” Morrison, M. J.; Imperiali, B. Arch. Biochem Biophys. 2013, 536, 72-80. PMC3722612 “Biochemical Analysis and Structure Determination of Bacterial Acetyltransferases Responsible for the Biosynthesis of UDP-N,N’-Diacetylbacillosamine” Morrison, M. J., Imperiali, B., J. Biol. Chem. 2013, 288, 32248-32260. PMC3820863. “Lipid Bilayer Nanodisc Platform for Investigating Polyprenol-Dependent Enzyme Interactions and Activities" Hartley, M. D.; Schneggenburger, P. E. Imperiali, B. Proc. Natl. Acad. Sci. USA. 2013, PMC3876266 bb “Two-Photon Fluorescence Spectroscopy and Imaging of 4-Dimethylamino-naphthalimide-Peptide and Protein Conjugates” McLean, A. M.; Socher, E.; Clark, T. B.; Imperiali, B.; Goodson, T. III, J. Phys. Chem. 2013, PMC3938489 2012 "At the Membrane Frontier: A Prospectus on the Remarkable Evolutionary Conservation of Polyprenols and Polyprenyl-phosphates" Hartley, M. D.; Imperiali, B. Arch. Biochem. Biophys. 2012, 517, 83-97. PMC3253937 bbbbbb "Interrogating Signaling Nodes Involved in Cellular Transformations Using Kinase Activity Probes" Stains, C. I.; Tedford, N. C.; Walkup, T. C.; Luković, E.; Goguen, B. N.; Griffith, L. G.; Lauffenburger, D. A.; Imperiali, B. Chemistry & Biology 2012, 19, 210-217. PMC3307342 bb “Tailoring Encodable Lanthanide-Binding Tags as MRI Contrast Agents” Daughtry, K. D.; Martin, L. M.; Sarraju, A.; Imperiali, B.; Allen, K. N. ChemBioChem 2012, 13, 2567-2574. DOI: 10.1002/cbic.201200448 2011 "A p38α-Selective Chemosensor for use in Unfractionated Cell Lysates" Stains, C. I.; Luković, E.; Imperiali, B. ACS Chemical Biology, 2011, 6, 101-105. PMC3025060 "Biomimetic Divalent Ligands for the Acute Disruption of AMPAR Stabilization" Sainlos, M.; Tigaret, C.; Poujol, C.; Olivier, N. B.; Bard, L.; Breillat, C.; Thiolon, K.; Choquet, D.; Imperiali, B. Nature Chemical Biology, 2011, 7, 81-91. doi: 10.1038/nchembio.498 "Engineering Encodable Lanthanide Binding Tags (LBTs) into Loop Regions of Proteins" Barthelmes, K.; Reynolds, A. M.; Peisach, E.; Jonker, H.; Allen, K. N.; Imperiali, B.; Schwalbe, H. J. Am. Chem. Soc. 2011, 133, 808-819. PMC3043167 "Light-Triggered Myosin Activation for Probing Dynamic Cellular Processes" Goguen, B. N.; Hoffman, B. D.; Sellers, J. R.; Schwartz, M. A.; Imperiali, B. Angew. Chem. Int. Ed. 2011, 50, 5667-5670. PMC3406609 "Biochemical Characterization of the O-linked Glycosylation Pathway in Neisseria gonorrhoeae Responsible for Biosynthesis of Pilin Glycans Containing N,N’-Diacetylbacillosamine" Hartley, M. D.; Morrison, M. J.; Aas, F. E.; Børud, B.; Koomey, M.; Imperiali, B. Biochemistry, 2011, 50, 4936-4948. PMC3108506 "Development of a Fluorogenic Sensor for Activated Cdc42" Goguen, B. N.; Loving, G. S.; Imperiali, B. Bioorg. Med. Chem. Lett. 2011, 21, 5058-5061. PMC3156282 "The Expanding Horizons of Asparagine-Linked Glycosylation" Larkin, A.; Imperiali, B. Biochemistry, 2011, 50, 4411-4426. PMC3101296 "Genetic and Molecular Analyses Reveal an Evolutionary Trajectory for Glycan Synthesis in a Bacterial Protein Glycosylation System" Børud, B.; Viburiene, R.; Hartley, M. D.; Paulsen, B. S.; Egge-Jacobsen, W.; Imperiali, B.; Koomey, M. Proc. Natl. Acad. Sci. USA, 2011,108, 9643-9648. PMC3111294 "Sequential Activation and Deactivation of Protein Function Using Spectrally Differentiated Caged Phosphoamino Acids" Goguen, B. N.; Aemissegger, A.; Imperiali, B. J. Am. Chem. Soc. 2011, 133, 11038-11041. PMC3146339 "Exploiting Topological Constraints to Reveal Buried Sequence Motifs in the Membrane-Bound N-Linked Oligosaccharyl Transferases” Jaffee, M. B.; Imperiali, B. Biochemistry, 2011, 50, 7557-7567. PMC3164389 "Chemical Tools for Studying Directed Cell Migration" Goguen, B. N. Imperiali, B. ACS Chemical Biology, 2011, 6, 1164-1174. PMC3220797 2010 "Lanthanide-Tagged Proteins – An Illuminating Partnership" Allen, K. N. Imperiali, B. Current Opin. Chem. Biol. 2010, 14, 247-254. "Structural Analysis of WbpE from Pseudomonas aeruginosa PAO1: A Nucleotide Sugar Aminotransferase Involved in O-antigen Assembly" Larkin, A.; Olivier, N. B.; Imperiali, B. Biochemistry, 2010, 49, 7227-7237. PMC2923267 "Modulation of Shank3 PDZ Domain Ligand-binding Affinity by Dimerization” Iskenderian-Epps, W. S.; Imperiali, B. ChemBioChem, 2010, 11, 1979-1984. DOI: 10.1002/cbic.201000246 "Dynamic and Specific Interaction Between Synaptic NR2-NMDA Receptor and PDZ Proteins" Bard, L.; Sainlos, M.; Cousins, S.; Mikasova, L.; Breillat, C.; Stephenson, F. A.; Imperiali, B.; Choquet, D.; Groc, L. Proc. Natl. Acad. Sci. USA 2010, 107(45), 19561-19566. PMC2984211 "Development of a Multicomponent Kinetcic Assay in the Early Enzymes in the Campylobacter jejuni N-Linked Glycosylation Pathway" Morrison, J. P.; Troutman, J. M.; and Imperiali, B. Bioorg. Med. Chem. 2010, 18(23), 8167-8171. PMC2981441 "Perturbing the Folding Energy Landscape of the Bacterial Immunity Protein Im7 by Site-Specific N-Linked Glycosylation" Chen, M. M.; Bartlett, A. I.; Nerenberg, P. S.; Friel, C. T.; Hackenberger, C. P. R.; Stultz, C. M.; Radford, S. E.; Imperiali, B. Proc. Natl. Acad. Sci. USA, 2010, 107(52), 22528-22533. PMC3012502 2009 "A Rapid Method for Generation of Selective Sox-based Chemosensors of Ser/Thr Kinases Using Combinatorial Peptide Libraries" González-Vera, J.A.; Luković, E.; Imperiali, B. Bioorg. Med. Chem. Lett. 2009, 19, 1258-1260. PMC2838374 "Campylobacter jejuni PglH is a Single Active Site Processive Polymerase that Utilizes Product Inhibition to Limit Sequential Glycosyl Transfer Reactions" Troutman, J.; Imperiali, B. Biochemistry, 2009, 48, 2807-2816. PMC2736683 “Biosynthesis of UDP-GlcNAc(3NAc)A by WbpB, WbpE, and WbpD: Enzymes in the Wbp Pathway Responsible for O-antigen Assembly in Pseudomonas aeruginosa PAO", Larkin, A.; Imperiali, B. Biochemistry, 2009, 48, 5446-5455. PMC2694220 "A General Screening Strategy for Peptide-Based Fluorogenic Ligands: Probes for Dynamic Studies of PDZ Domain-Mediated Interactions”, Sainlos, M.; Iskenderian, W. S.; Imperiali, B. J. Am. Chem. Soc. 2009, 131, 6680-6682. PMC2828873 "Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters", Lukovic, E.; Vogel Taylor, E.; Imperiali, B. Angew. Chem. Int. Ed. 2009, 48, 6828-6831. PMC2937354 "Synthesis of Red-shifted 8-Hydroxyquinoline Derivatives Using Click Chemistry and Their Incorporation into Phosphorylation Chemosensors" González-Vera, J. A.; Luković, E.; Imperiali, B. J. Org. Chem. 2009, 74, 7309-7314. PMC2762270 "Thiol-reactive Derivatives of the Solvatochromic 4-N,N-Dimethylamino-1,8-naphthalimide Fluorophore: A Highly Sensitive Toolset for the Detection of Biomolecular Interactions" Loving, G. S. Imperiali, B. Biocong. Chem. 2009, 20, 2133-2141. PMC2783863 "Monitoring Protein Interactions and Dynamics with Solvatochromic Fluorophores" Loving, G. S.; Sainlos, M.; Imperiali, B. Trends in Biotechnol. 2009, 28, 73-83. PMC2818466 2008 "Lanthanide-Binding Tags with Unnatural Amino Acids: Sensitizing Tb3+ and Eu3+ Luminescence at Longer Wavelengths" Reynolds, A. M.; Sculimbrene, B. R.; Imperiali, B. Bioconjugate Chem. 2008, 19, 588-591. doi: 10.1021/bc700426c "Chemoenzymatic Synthesis of Polyprenyl Phosphates" Hartley, M. D.; Larkin, A.; Imperiali, B. Bioorg. Med. Chem. 2008, 16, 5149-5156. PMC2471869 "Solution Structure of Alg13: The Sugar Donor Subunit of a Yeast N-Acetylglucosamine Transferase" Wang, X.; Weldeghorghis, T.; Zhang, G.; Imperiali, B.; Prestegard, J. H. Structure, 2008, 16, 965-975. PMC2486831 "Structure Determination of a Galectin-3 – Carbohydrate Complex Using Paramagnetism-based NMR Constraints" Zhuang, T.; Lee, H-S.; Imperiali, B.; Prestegard, J. H. Protein Science, 2008, 17, 1220-1231. PMC2442008 "Affinity-Capture Tandem Mass Spectrometric Characterization of Polyprenyl-linked Oligosaccharides: A Novel Tool to Study Protein N-Glycosylation Pathways" Reid, C. W.; Stupak, J.; Chen, M. M.; Imperiali, B.; Li, J.; Szymanski, C. M. Analytical Chem. 2008, 80, 5468-5475. PMC2763189 "Crystal Structure and Catalytic Mechanism of PglD from Campylobacter jejuni" Olivier, N. B.; Imperiali, B. J. Biol. Chem. 2008, 283, 27937-27946. PMC2562079 "Recognition-Domain Focused (RDF) Chemosensors: Versatile and Efficient Reporters of Protein Kinase Activity" Luković, E.; González-Vera, J. A.; Imperiali, B. J. Am. Chem. Soc. 2008, 130, 12821-12827. PMC2637182 "A Versatile Amino Acid Analogue of the Solvatochromic Fluorophore 4-N,N-Dimethylamino-1,8-naphthalimide: A Powerful Tool for the Study of Dynamic Protein Interactions" Loving, G. S.; Imperiali, B. J. Am. Chem. Soc. 2008, 130, 13630-13638. PMC2647014 2007 "Semisynthesis of Unnatural Amino Acid Mutants of Paxillin: Protein Probes for Cell Migration Studies" Vogel, E. M.; Imperiali, B. Protein Science 2007, 16, 550-556. PMC2203312 "Fluorogenic Probes for Monitoring Peptide Binding to Class II MHC Proteins in Living Cells" Venkatraman, P.; Nguyen, T.; Sainlos, M. Bilsel, O. Calvo-Calle, Chitta, M. S. Imperiali, B.; Stern, L. J. Nature Chemical Biology 2007, 3, 222-228. PMC3444530 "From Peptide to Protein: Comparative Analysis of the Substrate Specificity of N-Linked Glycosylation in C. jejuni" Chen, M. M.; Glover, K. J.; Imperiali, B. Biochemistry, 2007, 46, 5579-5585. "Caged O-Phosphorothioyl Amino Acids as Building Blocks for Fmoc-based Solid Phase Peptide Synthesis" Aemissegger, A.; Carrigan, C. N.; Imperiali, B. Tetrahedron, 2007, 63, 6185-6190. PMC2699315 "Native Chemical Ligation: Semisynthesis of Post-Translationally Modified Proteins and Biological Probes" Imperiali, B.: Vogel, E. M. in Protein Engineering, Köhrer, C.; RajBhandary, U. L. Eds. Springer 2009, Chapter 3, pp 65-96. "Double Lanthanide-Binding Tags: Design, Photophysical Properties and NMR Applications" Martin, L. J.; Hähnke, M. J.; Nitz, M.; Wöhnert, J.; Silvaggi, N. J.; Allen, K. N.; Schwalbe, H.; Imperiali, B. J. Am. Chem. Soc. 2007, 129, 7106-7113. "Double Lanthanide-Binding Tags for Macromolecular Crystallographic Structure Determination" Silvaggi, N. J.; Martin, L. J.; Schwalbe, H.; Imperiali, B.; Allen, K. N. J. Am. Chem. Soc. 2007, 129, 7114-7120. "Tools for Investigating Peptide-Protein Interactions: Peptide Incorporation of Environment-Sensitive Fluorophores via on Resin Derivatization" Sainlos, M.; Imperiali, B. Nature Protocols 2007, 2, 3201-3209. "Tools for Investigating Peptide-Protein Interactions: Peptide Incorporation of Environment-Sensitive Fluorophores through SPPS-based ‘Building Block’ Approach" Sainlos, M.; Imperiali, B. Nature Protocols, 2007, 2, 3210-3218. "Synthesis of Anhydride Precursors of the Environment-Sensitive Fluorophores 4-DMAP and 6-DMN" Sainlos, M.; Imperiali, B. Nature Protocols, 2007, 2, 3219-3225. "Polyisoprene Specificity in the Campylobacter jejuni N-linked Glycosylation Pathway" Chen, M. M; Weerapana, E.; Ciepichal, E.; Stupak, J.; Reid, C. W.; Swiezewska, E.; Imperiali, B. Biochemistry, 2007, 46, 14342-14348. PMC2585822 2006 "Asparagine-linked Protein Glycosylation: From Eukaryotic to Prokaryotic Systems" Weerapana, E.; Imperiali, B. Glycobiology, 2006, 16, 91R-101R. "Optimal Sox-based Fluorescence Chemosensor Design for Serine/Threonine Protein Kinases" Shults, M. D.; Carrico-Moniz, D.; Imperiali, B. Analytical Biochem. 2006, 352, 198-207. "Expression of N-Terminal Cys-Protein Fragments using an Intein Refolding Strategy" Hackenberger, C. P. R.; Chen, M. M.; Imperiali, B. Bioorg. Med. Chem. 2006, 14, 5043-5048. "Direct Biochemical Evidence for the Utilization of UDP-Bacillosamine by PglC, an Essential Glycosyl-1-phosphate-transferase in the C. jejuni N-linked Glycosylation Pathway" Glover, K. J.; Weerapana, E.; Chen, M. M.; Imperiali, B. Biochemistry, 2006, 45, 5343-5350. "Lanthanide Binding Tags as Luminescent Probes for Studying Protein Interactions" Sculimbrene, B. R.; Imperiali, B. J. Am. Chem. Soc. 2006, 128, 7346-7352. "In vitro Evidence for the Dual Function of Alg2 and Alg11: Essential Mannosyltransferases in N-linked Glycoprotein Biosynthesis" O’Reilly, M. K.; Zhang, G.; Imperiali, B. Biochemistry, 2006, 45, 9593-9603. "In vitro biosynthesis of UDP-N,N’-diacetylbacillosamine by Enzymes of the Campylobacter jejuni General Protein Glycosylation System" Olivier, N. B.; Chen, M. M.; Behr, J. R.; Imperiali, B. Biochemistry 2006, 45, 13659-13669. PMC2542654 2005 "Caged Phosphoproteins," Rothman, D. M.; Petersson, E. J.; Vázquez, M. E.; Brandt, G. S.; Dougherty, D. A.; Imperiali, B. J. Am. Chem. Soc. 2005, 127, 846-847. "Photophysics and Biological Applications of the Environment-Sensitive Fluorophore 6-N,N-Dimethylamino-2,3-Naphthalimide" Vázquez, M. E.; Canosa, J. B. B.; Imperiali, B. J. Am. Chem. Soc. 2005, 127, 1300-1306. "Design of a Heterospecific, Tetrameric, 21-Residue Miniprotein with Mixed α/β Structure" Ali, M. H.; Taylor, C. M.; Grigoryan, G.; Allen, K. N.; Imperiali, B.; Keating, A. E. Structure, 2005, 13, 225-234. "A Multiplexed Homogenous Fluorescence-Based Assay for Protein Kinase Activity in Cell Lysates," Shults, M. D.; Janes, K. A.; Lauffenburger, D. A.; Imperiali, B. Nature Methods, 2005, 2, 277-284. "Improving Glycopeptide Synthesis: A Convenient Protocol for the Preparation of β−Glycosylamines and the Synthesis of Glycopeptides" Hackenberger, C. P. R.; O’Reilly, M. O.; Imperiali, B. J. Org. Chem. 2005, 70, 3574-3578. "The Engineering of Membrane-Permeable Peptides" Carrigan, C. N.; Imperiali, B., Analytical Biochem. 2005, 341, 290-298. "In situ Photoactivation of a Caged Phosphotyrosine Peptide Derived from Focal Adhesion Kinase Temporarily Halts Lamellar Extension of Single Migrating Tumor Cells" David Humphrey, D.; Rajfur, Z.; Vazquez, M. E.; Scheswohl, D.; Schaller, M. D.; Jacobson, K.; Imperiali, B. J. Biol. Chem. 2005, 280, 22091-22101. "Protein Oligomerization: How and Why" Ali, M. H.; Imperiali, B. Bioorg. Med. Chem. 2005, 13, 5013-5020. "Chemical Approaches for Investigating Phosphorylation in Signal Transduction Networks" Rothman, D. M.; Shults, M. D.; Imperiali, B. Trends Cell. Biol. 2005, 15, 502-510. "Semi-synthesis of a Glycosylated Im7 Analogue for Protein Folding Studies" Hackenberger, C. P. R; Friel, C. T.; Radford, S. E.; Imperiali, B. J. Am. Chem. Soc. 2005, 127, 12882-12889. PMC1356972 "In vitro Assembly of the Undecaprenylpyrophosphate-Linked Heptasaccharide for Prokaryotic N-linked Glycosylation" Glover, K. J.; Weerapana, E.; Imperiali, B. Proc. Natl. Acad. Sci. USA, 2005, 102, 14255-14259. PMC1242339 "Investigating Bacterial N-linked Glycosylation: Synthesis and Glycosyl Acceptor Activity of Undecaprenylpyrophosphate-linked Bacillosamine" Weerapana, E.; Glover, K. J.; Chen, M. M.; Imperiali, B. J. Am. Chem. Soc. 2005, 127, 13766-13767. PMC1351108 "Rapid Combinatorial Screening of Peptide Libraries for the Selection of Lanthanide-Binding Tags (LBTs)" Martin, L. J.; Sculimbrene, B. R.; Nitz, M.; Imperiali, B. QSAR Comb. Sci. 2005, 10, 1149-1157. "Chemoenzymatic Synthesis of Glycopeptides using PglB, a Bacterial Oligosaccharyl Transferase from Campylobacter jejuni " Glover, K. J.; Weerapana, E.; Imperiali, B. Chemistry & Biology 2005, 12, 1311-1316. PMC2519243 "Application of Photoactivation and CALI (Chromophore-Assisted Light Inactivation) Technologies to Problems in Cell Motility," Humphrey, D.; Rajfur, Z.; Imperiali, B.; Marriott, G.; Roy, P.; Jacobson, K. in Live Cell Imaging: A Laboratory Manual, Spector, D. L.; Goldman, R. D. Eds. Cold Spring Harbor Laboratory Press (2005), Chapter 10, pp 159-176. 2004 "Structural Origin of the High Affinity of a Chemically Evolved Lanthanide Binding Peptide," Nitz, M.; Sherawat, M.; Franz, K. J.; Peisach, E.; Allen, K. N.; Imperiali, B. Angew. Chem. Int. Ed. 2004, 43, 3682-3685. "Effects of Glycosylation on Peptide Conformation: A Synergistic Experimental and Computational Study," Bosques, C. J.; Tschampel, S. M.; Woods, R. J.; Imperiali, B. J. Am. Chem. Soc. 2004, 126, 8421-8425. PMC1386730 "Caged Phosphopeptides Reveal a Temporal Role for 14-3-3 in G1 Arrest and S-Phase Checkpoint Function," Nguyen, A.; Rothman, D. M.; Stehn, J.; Imperiali, B.; Yaffe, M. B. Nature Biotechnology, 2004, 22, 993-1000. "X-Ray Structure Analysis of a Designed Oligomeric Mini-Protein Reveals a Discrete Quaternary Architecture," Ali, M. H.; Peisach, E.; Allen, K. N.; Imperiali, B. Proc. Natl Acad. Sci. USA, 2004, 101, 12183-12188. PMC514454 "A New Environment-Sensitive Fluorescent Amino Acid for Fmoc-based Solid Phase Peptide Synthesis," Vázquez, M. E.; Rothman, D. M.; Imperiali, B. Org. Biomol. Chem. 2004, 2, 1965-1966. "Heterologous Expression and Biophysical Characterization of Soluble Oligosaccharyl Transferase Subunits," Dempski, R. E. Jr.; Imperiali, B. Arch. Biochem. Biophys. 2004, 431, 63-70. 2003 "Peptides to Peptidomimetics: Towards the Design and Synthesis of Bioavailable Inhibitors of Oligosaccharyl Transferase," Weerapana, E.; Imperiali, B. Org. Biomol. Chem. 2003, 1, 93-99. "Lanthanide-Binding Tags as Versatile Protein Coexpression Probes," Franz, K. J.; Nitz, M.; Imperiali, B. ChemBioChem. 2003, 4, 265-271. "A Powerful Combinatorial Screen to Identify High-Affinity Terbium(III)-Binding Peptides," Nitz, M.; Franz, K. J.; Maglathlin, R. L.; Imperiali, B. ChemBioChem. 2003, 4, 272-276. "A Chemist’s Approach to Biochemical Complexity,” Aldridge, S.; Imperiali, B. Chem. Comm. 2003, 445-447. "The Interplay of Glycosylation and Disulfide Formation Influences Fibrillization in a Prion Protein Fragment," Bosques, C. J.; Imperiali, B. Proc. Natl. Acad. Sci. USA 2003, 100, 7593-7598. PMC164631 "Chemistry and Biochemistry of Asparagine-Linked Protein Glycosylation," Imperiali, B.; Tai, V. W-F. in "Carbohydrate-based Drug Discovery"; Wong, C. H., ed. Wiley-VCH Verlag GmbH Publisher: Weinheim, 2003, pp 281-303. "Photolytic Control of Peptide Self-Assembly," Bosques, C. J.; Imperiali, B. J. Am. Chem. Soc. 2003, 125, 7530-7531. "Fluorescent Caged Phosphoserine Peptides as Probes to Investigate Phosphate-Dependent Protein Associations," Vázquez, M. E.; Nitz, M.; Stehn, J.; Yaffe, M. B. Imperiali, B. J. Am. Chem. Soc. 2003, 125, 10150-10151. "A Modular and Tunable Chemosensor Scaffold for Divalent Zinc," Shults, M. D.; Pearce, D. A.; Imperiali, B. J. Am. Chem. Soc. 2003, 125, 10591-10597. "Caged Phospho-Amino Acid Building Blocks Amenable to Fmoc-based Solid Phase Peptide Synthesis," Rothman, D. M.; Vázquez, M. E.; Vogel, E. M.; Imperiali, B. J. Org. Chem. 2003, 68, 6795-6798. "Protein Alignment by a Coexpressed Lanthanide-Binding Tag for the Measurement of Residual Dipolar Couplings," Wöhnert, J.; Franz, K. J.; Nitz, M.; Imperiali, B.; Schwalbe, H. S. J. Am. Chem. Soc. 2003, 125, 13338-13339. "Versatile Fluorescence Probes of Protein Kinase Activity," Shults, M. D.; Imperiali, B. J. Am. Chem. Soc. 2003, 125, 14248-14249. 2002 "Oligomeric ββα Mini-Protein Motifs: Pivotal Role of Single Hinge Residue in Determining the Oligomeric State," McDonnell, K. A.; Imperiali, B. J. Am. Chem. Soc. 2002, 124, 428-433. "A General Method for the Synthesis of Caged Phosphopeptides: Tools for the Exploration of Signal Transduction Pathways," Rothman, D. M.; Vázquez, M. E.; Vogel, E. M.; Imperiali, B. Org. Lett. 2002, 4, 2865-2868. "Enantioselective Synthesis and Application of the Highly Fluorescent and Environment-Sensitive Amino Acid 6-(2-dimethylaminonaphthoyl) Alanine (DANA)," Nitz, M.; Mezo, A. R.; Ali, M. H.; Imperiali, B. Chem. Comm. 2002, 1912-1913. "Neoglycopeptides as Inhibitors of Oligosaccharyl Transferase: Insight into Negotiating Product Inhibition," Peluso, S.; Ufret, M. de L.; O'Reilly, M. K.; Imperiali, B. Chemistry & Biology 2002, 9, 1323-1328. "Oligosaccharyl Transferase: Gatekeeper to the Secretory Pathway," Dempski, R. E. Jr.; Imperiali, B. Curr. Op. Chem. Biol. 2002, 6, 844-850. 2001 "Design of a Discretely Folded Mini-Protein Motif with Predominantly β-Structure," Ottesen, J. J.; Imperiali, B. Nature Structural Biology, 2001, 8, 535-539. "Substrate Specificity of N-Acetylglucosaminyl(diphosphodolichol) N-Acetylglucosaminyl Transferase, a Key Enzyme in the Dolichol Pathway," Tai, V. W.-F.; O’Reilly, M. K.; Imperiali, B. Bioorg. Med. Chem. 2001, 9, 1133-1140. “Discovery and Characterization of a Discretely Folded Homotrimeric ββα Peptide,” Mezo, A. R.; Ottesen, J. J.; Imperiali, B. J. Am. Chem. Soc. 2001, 123, 1002-1003. "Derivatives of 8-Hydroxy-2-methyl-quinoline are Powerful Prototypes for Zinc Sensors in Biological Systems," Pearce, D. P.; Jotterand, N.; Carrico, I. S.; Imperiali, B. J. Am. Chem. Soc. 2001, 123, 5160-5161. "Asymmetric Synthesis of a New 8-Hydroxyquinoline Derived α-Amino Acid and its Incorporation in a Peptidyl Sensor for Divalent Zinc," Jotterand, N.; Pearce, D. A.; Imperiali, B. J. Org. Chem. 2001, 66, 3224-3228. "Oligomerization of Uniquely Folded Mini-Protein Motifs: Development of a Homotrimeric ββα Peptide," Mezo, A. R.; Cheng, R. P.; Imperiali, B. J. Am. Chem. Soc. 2001, 123, 3885-3891. "Asparagine Surrogates for the Assembly of N-linked Glycopeptide Mimetics by Chemoselective Ligation," Peluso, S.; Imperiali, B. Tetrahedron Lett. 2001, 42, 2085-2087. "Probing the Effect of the Outer Saccharide Residues of N-Linked Glycans on Peptide Conformation," O'Connor, S. E.; Pohlmann, J.; Imperiali, B.; Saskiawan, I.; Yamamoto, K. J. Am. Chem. Soc. 2001, 123, 6187-6188. "Substrate Specificity for the Glycosyl Donor of Oligosaccharyl Transferase," Tai, V. W.-F.; Imperiali, B. J. Org. Chem. 2001, 66, 6217-6228. "Stereoselective Synthesis of β−Linked TBDMS-Protected Chitobiose-Asparagine: A Versatile Building Block for Amyloidogenic Glycopeptides," Bosques, C. J.; Tai, V. W.-F.; Imperiali, B. Tetrahedron Lett. 2001, 42, 7207-7210.

2017

“Targeting Bacillosamine Biosynthesis in Bacterial Pathogens: Development of Inhibitors to a Bacterial Amino-Sugar Acetyl Transferase from Campylobacter jejuni”, De Schutter, J. W.; Morrison, J. P.; Morrison, M. J.; Ciulli, A.; Imperiali, B. J. Med. Chem 2017, 60(5), 2099-2118. PMC5506375

“Conformational Dynamics and Alignment Properties of Loop Lanthanide-Binding Tags (LBTs) Studied in Interleukin-1β”, Schwalbe, H.; Barthelmes, D.; Barthelmes, K.; Schnorr, K.; Hendrik R. A.; Jonker B. B.; Allen, K. N.; Imperiali, B. J. Biol NMR, 2017, May 22. DOI:10.1007/s10858-017-0118-5.

“Analysis of a Dual Domain Phosphoglycosyl Transferase Reveals a Ping-Pong Mechanism with a Covalent Enzyme Intermediate”, Das, D.; Suzmic, P.; Imperiali, B. Proc. Natl. Acad. Sci. USA 2017, 114(27): 7019-7024. PMC5502628

“Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars”, Zamora, C.; Schocker, N. S.; Chang, M. M.; Imperiali, B. Meth. Enzymol. 2017, in press.

“Bacterial Phosphoglycosyl Transferases: Initiators of Glycan Biosynthesis at the Membrane Interface”, Lukose, V.; Walvoort, M. T. C.; Imperiali, B. Glycobiology, 2017, in press.

2016

“A Modular Approach to Phosphoglycosyl Transferase Inhibitors Inspired by Nulceoside Antibiotics” Walvoort, M. T. C.; Lukose, V.; Imperiali, B. Chem. Eur. J. 2016, 22, 3856-3864. PMC5506376

“Probing Polytopic Membrane Protein - Substrate Interactions by Luminescence Resonance Energy Transfer” Musial-Siwek, M.; Jafee, M. B.; Imperiali, B. J. Am. Chem. Soc. 2016, 3806-3812. PMC5480290

“Design Principles for SuCESsFul Biosensors: Specific Fluorophore/Analyte Binding and Minimization of Fluorophore/Scaffold Interactions”. de Picciotto, S.; Dickson, P. M.; Traxlmayr, M. W.; Marques, B. S.; Socher, E.; Zhao, S.; Cheung, S.; Kiefer, J.; Griffith, L. G.; Wand, A. J.; Imperiali, B.; Wittrup, K. D. J. Mol. Biol. 2016, 428, 2228-2241. PMC5048519 DOI: 10.1016/j.jmb.2016.07.004

“A Rapid and Efficient Luminescence-based Method for Assaying Phosphoglycosyl-transferase Enzymes” Das, D.; Walvoort, M. T. C.; Lukose, V.; Imperiali, B. Scientific Reports, 2016, 6:33412. PMC5022061.

“Bacterial N-Glycosylation Efficiency is Dependent on the Structural Context of Target Sequons” Silverman, J. M.; Imperiali, B. J. Biol. Chem. 2016, 291, 22001-22010. PMC5063983 doi:10.1074/jbc.M116.747121.

“Antibodies from Multiple Sclerosis Patients Preferentially Recognize Hyperglucosylated Adhesin of Non-Typeable Haemophilus influenzae“, Walvoort, M. T. C.; Testa, C.; Eilam, R.; Aharoni, R.; Nuti, F.; Rossi, G.; Real-Fernandez, R.; Lanzillo, R.; Morra, V. B.; Lolli, F.; Rovero, P.; Imperiali, B.; Papini, A-M. Scientific Reports, 2016, 6:39430. PMC5180199

2015

“The Best and the Brightest: Exploiting Tryptophan-Sensitized Tb3+ Luminescence to Engineer Lanthanide-Binding Tags” Martin, L. J.; Imperiali, B. Methods in Molecular Biology 2015, 1248, 201-220. ISBN 978-1-4939-2019-8.

”Selective Biochemical Labeling of C. jejuni Cell-Surface Glycoconjugates” Whitworth, G.; Imperiali, B. Glycobiology 2015, 25, 756-766. PMC25761366

”N-Linked Glycans are Assembled on Highly Reduced Dolichol Phosphate Carriers in the Hyperthermophilic Archaea Pyrococcus furiosus,” Chang, M. M; Imperiali, B.; Eichler, J; Guan, Z. PLoS One 2015, 10 6 e0130482. PMC4476749

“Covalent Modification Of Synthetic Hydrogels With Bioactive Proteins Via Sortase-Mediated Ligation“ Cambria, E.; Renggli, K.; Ahrens, C. C.; Cook, C. D.; Kroll, C.; Krueger, A. T.; Imperiali, B.; Griffith, L. G. Biomacromolecules, 2015, 16, 2316-2326. PMC4613866

“Encoded Loop-Lanthanide-Binding Tags for Long-Range Distance Measurements in Proteins by NMR and EPR Spectroscopy” Barthelmes, D.; Granz, M.; Barthelmes, K.; Allen, K. N.; Imperiali, B.; Prisner, T.; and Schwalbe, H. J. Biomol. NMR. 2015, doi: 10.1007/s10858-015-9984-x.

“Chemoenzymatic Assembly of Bacterial Glycoconjugates for Site-Specific Orthogonal Labeling” Lukose, V; Whitworth, G; Guan, Z.; Imperiali, B. J. Am. Chem. Soc. 2015, 137, 12446-12449. PMC4599313.

“Conservation and Covariance in Monotopic Phosphoglycosyltransferases identifies the Functional Catalytic Core” Lukose, V.; Luo, L.; Kozakov, D.; Vajda, S.; Allen, K. N.; Imperiali, B. Biochemistry 2015, PMC5483379

2014

“The Renaissance of Bacillosamine and Its Derivatives: Pathway Characterization and Implications in Pathogenicity” Morrison, M. J. Imperiali, B. Biochemistry, 2014, 53, 624-638. PMC3951908

“Tailoring Chimeric Ligands for Studying and Biasing ErbB Receptor Family Interactions" Krueger, A. T.; Kroll, C.; Sanchez, E.; Griffith, L. G.; Imperiali, B. Angew. Chem. Int. Ed. 2014, 53, 2662-2666. PMC4018821

“Equilibrium and Dynamic Design Principles for Binding Molecules Engineered for Reagentless Biosensors” de Picciotto, S.; Imperiali, B.; Griffith, L. G.; Wittrup, K. D. Analytical Biochem. 2014, 460, 9-15. PMC4104283

“Selective Mitogen Activated Protein Kinase Activity Sensors through the Application of Directionally-Programmable D Domain Motifs” Peterson, L. B.; Yaffe, M. B.; Imperiali, B. Biochemistry 2014, 53, 5771-5778. PMC4165445

“Quantification of Protein Kinase Enzymatic Activity in Unfractionated Cell Lysates Using CSox-Based Sensors“ Beck, J. R.; Peterson, L. B.; Imperiali, B.; Stains, C. I. Curr. Protoc. Chem. Biol. 2014, 6, 135-156. PMC4174361

Peterson, L. B.; Imperiali, B. (2014) Real-Time and Continuous Sensors of Protein Kinase Activity Utilizing Chelation-Enhanced Fluorescence. Book Chapter in “Concepts and Case Studies in Chemical Biology”, (Edited by H. Waldmann and P. Janning) ISBN: 978-3-527-33611-1.

2013

"FRET-CAPTURE: A Sensitive Method for the Detection of Dynamic Protein Interactions” Socher, E.; Imperiali B. ChemBioChem 2013, 14, 53-57. PMC3776414

"Caged Mono- and Divalent Ligands for Light-Assisted Disruption of PDZ Domain-Mediated Interactions” Sainlos, M.; Iskenderian-Epps, W. S.; Olivier, N. B.; Choquet, D.; Imperiali B. J. Am. Chem. Soc. 2013, 135(12), 4580-4583. doi: 10.1021/ja309870q

"Biochemical Evidence for an Alternate Pathway in N-linked Glycoprotein Biosynthesis" Larkin, A.; Chang, M. M.; Whitworth, G.E.; Imperiali, B. Nature Chemical Biology, 2013, 9, 367-373. PMC3661703

"Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology” Krueger, A. T.; Imperiali B. ChemBioChem, 2013, 14, 788-799. DOI: 10.1002/cbic.201300079

"Optimized Protocol for Expression and Purification of PglB the Membrane-Bound Bacterial Oligosaccharyl Transferase" Jaffee, M. B.; Imperiali, B. Protein Expression & Purification, 2013, 89, 241-250. PMC3690457

“Biosynthesis of UDP-N,N’Diacetylbacillosamine in Acinetobacter baumannii: Biochemical Characterization and Correlation to Existing Pathways” Morrison, M. J.; Imperiali, B. Arch. Biochem Biophys. 2013, 536, 72-80. PMC3722612

“Biochemical Analysis and Structure Determination of Bacterial Acetyltransferases Responsible for the Biosynthesis of UDP-N,N’-Diacetylbacillosamine” Morrison, M. J., Imperiali, B., J. Biol. Chem. 2013, 288, 32248-32260. PMC3820863.

“Lipid Bilayer Nanodisc Platform for Investigating Polyprenol-Dependent Enzyme Interactions and Activities" Hartley, M. D.; Schneggenburger, P. E. Imperiali, B. Proc. Natl. Acad. Sci. USA. 2013, PMC3876266

“Two-Photon Fluorescence Spectroscopy and Imaging of 4-Dimethylamino-naphthalimide-Peptide and Protein Conjugates” McLean, A. M.; Socher, E.; Clark, T. B.; Imperiali, B.; Goodson, T. III, J. Phys. Chem. 2013, PMC3938489

2012

"At the Membrane Frontier: A Prospectus on the Remarkable Evolutionary Conservation of Polyprenols and Polyprenyl-phosphates" Hartley, M. D.; Imperiali, B. Arch. Biochem. Biophys. 2012, 517, 83-97. PMC3253937

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"Interrogating Signaling Nodes Involved in Cellular Transformations Using Kinase Activity Probes" Stains, C. I.; Tedford, N. C.; Walkup, T. C.; Luković, E.; Goguen, B. N.; Griffith, L. G.; Lauffenburger, D. A.; Imperiali, B. Chemistry & Biology 2012, 19, 210-217. PMC3307342

“Tailoring Encodable Lanthanide-Binding Tags as MRI Contrast Agents” Daughtry, K. D.; Martin, L. M.; Sarraju, A.; Imperiali, B.; Allen, K. N. ChemBioChem 2012, 13, 2567-2574. DOI: 10.1002/cbic.201200448

2011

"A p38α-Selective Chemosensor for use in Unfractionated Cell Lysates" Stains, C. I.; Luković, E.; Imperiali, B. ACS Chemical Biology, 2011, 6, 101-105. PMC3025060

"Biomimetic Divalent Ligands for the Acute Disruption of AMPAR Stabilization" Sainlos, M.; Tigaret, C.; Poujol, C.; Olivier, N. B.; Bard, L.; Breillat, C.; Thiolon, K.; Choquet, D.; Imperiali, B. Nature Chemical Biology, 2011, 7, 81-91. doi: 10.1038/nchembio.498

"Engineering Encodable Lanthanide Binding Tags (LBTs) into Loop Regions of Proteins" Barthelmes, K.; Reynolds, A. M.; Peisach, E.; Jonker, H.; Allen, K. N.; Imperiali, B.; Schwalbe, H. J. Am. Chem. Soc. 2011, 133, 808-819. PMC3043167

"Light-Triggered Myosin Activation for Probing Dynamic Cellular Processes" Goguen, B. N.; Hoffman, B. D.; Sellers, J. R.; Schwartz, M. A.; Imperiali, B. Angew. Chem. Int. Ed. 2011, 50, 5667-5670. PMC3406609

"Biochemical Characterization of the O-linked Glycosylation Pathway in Neisseria gonorrhoeae Responsible for Biosynthesis of Pilin Glycans Containing N,N’-Diacetylbacillosamine" Hartley, M. D.; Morrison, M. J.; Aas, F. E.; Børud, B.; Koomey, M.; Imperiali, B. Biochemistry, 2011, 50, 4936-4948. PMC3108506

"Development of a Fluorogenic Sensor for Activated Cdc42" Goguen, B. N.; Loving, G. S.; Imperiali, B. Bioorg. Med. Chem. Lett. 2011, 21, 5058-5061. PMC3156282

"The Expanding Horizons of Asparagine-Linked Glycosylation" Larkin, A.; Imperiali, B. Biochemistry, 2011, 50, 4411-4426. PMC3101296

"Genetic and Molecular Analyses Reveal an Evolutionary Trajectory for Glycan Synthesis in a Bacterial Protein Glycosylation System" Børud, B.; Viburiene, R.; Hartley, M. D.; Paulsen, B. S.; Egge-Jacobsen, W.; Imperiali, B.; Koomey, M. Proc. Natl. Acad. Sci. USA, 2011,108, 9643-9648. PMC3111294

"Sequential Activation and Deactivation of Protein Function Using Spectrally Differentiated Caged Phosphoamino Acids" Goguen, B. N.; Aemissegger, A.; Imperiali, B. J. Am. Chem. Soc. 2011, 133, 11038-11041. PMC3146339

"Exploiting Topological Constraints to Reveal Buried Sequence Motifs in the Membrane-Bound N-Linked Oligosaccharyl Transferases” Jaffee, M. B.; Imperiali, B. Biochemistry, 2011, 50, 7557-7567. PMC3164389

"Chemical Tools for Studying Directed Cell Migration" Goguen, B. N. Imperiali, B. ACS Chemical Biology, 2011, 6, 1164-1174. PMC3220797

2010

"Lanthanide-Tagged Proteins – An Illuminating Partnership" Allen, K. N. Imperiali, B. Current Opin. Chem. Biol. 2010, 14, 247-254.

"Structural Analysis of WbpE from Pseudomonas aeruginosa PAO1: A Nucleotide Sugar Aminotransferase Involved in O-antigen Assembly" Larkin, A.; Olivier, N. B.; Imperiali, B. Biochemistry, 2010, 49, 7227-7237. PMC2923267

"Modulation of Shank3 PDZ Domain Ligand-binding Affinity by Dimerization” Iskenderian-Epps, W. S.; Imperiali, B. ChemBioChem, 2010, 11, 1979-1984. DOI: 10.1002/cbic.201000246

"Dynamic and Specific Interaction Between Synaptic NR2-NMDA Receptor and PDZ Proteins" Bard, L.; Sainlos, M.; Cousins, S.; Mikasova, L.; Breillat, C.; Stephenson, F. A.; Imperiali, B.; Choquet, D.; Groc, L. Proc. Natl. Acad. Sci. USA 2010, 107(45), 19561-19566. PMC2984211

"Development of a Multicomponent Kinetcic Assay in the Early Enzymes in the Campylobacter jejuni N-Linked Glycosylation Pathway" Morrison, J. P.; Troutman, J. M.; and Imperiali, B. Bioorg. Med. Chem. 2010, 18(23), 8167-8171. PMC2981441

"Perturbing the Folding Energy Landscape of the Bacterial Immunity Protein Im7 by Site-Specific N-Linked Glycosylation" Chen, M. M.; Bartlett, A. I.; Nerenberg, P. S.; Friel, C. T.; Hackenberger, C. P. R.; Stultz, C. M.; Radford, S. E.; Imperiali, B. Proc. Natl. Acad. Sci. USA, 2010, 107(52), 22528-22533. PMC3012502

2009

"A Rapid Method for Generation of Selective Sox-based Chemosensors of Ser/Thr Kinases Using Combinatorial Peptide Libraries" González-Vera, J.A.; Luković, E.; Imperiali, B. Bioorg. Med. Chem. Lett. 2009, 19, 1258-1260. PMC2838374

"Campylobacter jejuni PglH is a Single Active Site Processive Polymerase that Utilizes Product Inhibition to Limit Sequential Glycosyl Transfer Reactions" Troutman, J.; Imperiali, B. Biochemistry, 2009, 48, 2807-2816. PMC2736683

“Biosynthesis of UDP-GlcNAc(3NAc)A by WbpB, WbpE, and WbpD: Enzymes in the Wbp Pathway Responsible for O-antigen Assembly in Pseudomonas aeruginosa PAO", Larkin, A.; Imperiali, B. Biochemistry, 2009, 48, 5446-5455. PMC2694220

"A General Screening Strategy for Peptide-Based Fluorogenic Ligands: Probes for Dynamic Studies of PDZ Domain-Mediated Interactions”, Sainlos, M.; Iskenderian, W. S.; Imperiali, B. J. Am. Chem. Soc. 2009, 131, 6680-6682. PMC2828873

"Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters", Lukovic, E.; Vogel Taylor, E.; Imperiali, B. Angew. Chem. Int. Ed. 2009, 48, 6828-6831. PMC2937354

"Synthesis of Red-shifted 8-Hydroxyquinoline Derivatives Using Click Chemistry and Their Incorporation into Phosphorylation Chemosensors" González-Vera, J. A.; Luković, E.; Imperiali, B. J. Org. Chem. 2009, 74, 7309-7314. PMC2762270

"Thiol-reactive Derivatives of the Solvatochromic 4-N,N-Dimethylamino-1,8-naphthalimide Fluorophore: A Highly Sensitive Toolset for the Detection of Biomolecular Interactions" Loving, G. S. Imperiali, B. Biocong. Chem. 2009, 20, 2133-2141. PMC2783863

"Monitoring Protein Interactions and Dynamics with Solvatochromic Fluorophores" Loving, G. S.; Sainlos, M.; Imperiali, B. Trends in Biotechnol. 2009, 28, 73-83. PMC2818466

2008

"Lanthanide-Binding Tags with Unnatural Amino Acids: Sensitizing Tb3+ and Eu3+ Luminescence at Longer Wavelengths" Reynolds, A. M.; Sculimbrene, B. R.; Imperiali, B. Bioconjugate Chem. 2008, 19, 588-591. doi: 10.1021/bc700426c

"Chemoenzymatic Synthesis of Polyprenyl Phosphates" Hartley, M. D.; Larkin, A.; Imperiali, B. Bioorg. Med. Chem. 2008, 16, 5149-5156. PMC2471869

"Solution Structure of Alg13: The Sugar Donor Subunit of a Yeast N-Acetylglucosamine Transferase" Wang, X.; Weldeghorghis, T.; Zhang, G.; Imperiali, B.; Prestegard, J. H. Structure, 2008, 16, 965-975. PMC2486831

"Structure Determination of a Galectin-3 – Carbohydrate Complex Using Paramagnetism-based NMR Constraints" Zhuang, T.; Lee, H-S.; Imperiali, B.; Prestegard, J. H. Protein Science, 2008, 17, 1220-1231. PMC2442008

"Affinity-Capture Tandem Mass Spectrometric Characterization of Polyprenyl-linked Oligosaccharides: A Novel Tool to Study Protein N-Glycosylation Pathways" Reid, C. W.; Stupak, J.; Chen, M. M.; Imperiali, B.; Li, J.; Szymanski, C. M. Analytical Chem. 2008, 80, 5468-5475. PMC2763189

"Crystal Structure and Catalytic Mechanism of PglD from Campylobacter jejuni" Olivier, N. B.; Imperiali, B. J. Biol. Chem. 2008, 283, 27937-27946. PMC2562079

"Recognition-Domain Focused (RDF) Chemosensors: Versatile and Efficient Reporters of Protein Kinase Activity" Luković, E.; González-Vera, J. A.; Imperiali, B. J. Am. Chem. Soc. 2008, 130, 12821-12827. PMC2637182

"A Versatile Amino Acid Analogue of the Solvatochromic Fluorophore 4-N,N-Dimethylamino-1,8-naphthalimide: A Powerful Tool for the Study of Dynamic Protein Interactions" Loving, G. S.; Imperiali, B. J. Am. Chem. Soc. 2008, 130, 13630-13638. PMC2647014

2007

"Semisynthesis of Unnatural Amino Acid Mutants of Paxillin: Protein Probes for Cell Migration Studies" Vogel, E. M.; Imperiali, B. Protein Science 2007, 16, 550-556. PMC2203312

"Fluorogenic Probes for Monitoring Peptide Binding to Class II MHC Proteins in Living Cells" Venkatraman, P.; Nguyen, T.; Sainlos, M. Bilsel, O. Calvo-Calle, Chitta, M. S. Imperiali, B.; Stern, L. J. Nature Chemical Biology 2007, 3, 222-228. PMC3444530

"From Peptide to Protein: Comparative Analysis of the Substrate Specificity of N-Linked Glycosylation in C. jejuni" Chen, M. M.; Glover, K. J.; Imperiali, B. Biochemistry, 2007, 46, 5579-5585.

"Caged O-Phosphorothioyl Amino Acids as Building Blocks for Fmoc-based Solid Phase Peptide Synthesis" Aemissegger, A.; Carrigan, C. N.; Imperiali, B. Tetrahedron, 2007, 63, 6185-6190. PMC2699315

"Native Chemical Ligation: Semisynthesis of Post-Translationally Modified Proteins and Biological Probes" Imperiali, B.: Vogel, E. M. in Protein Engineering, Köhrer, C.; RajBhandary, U. L. Eds. Springer 2009, Chapter 3, pp 65-96.

"Double Lanthanide-Binding Tags: Design, Photophysical Properties and NMR Applications" Martin, L. J.; Hähnke, M. J.; Nitz, M.; Wöhnert, J.; Silvaggi, N. J.; Allen, K. N.; Schwalbe, H.; Imperiali, B. J. Am. Chem. Soc. 2007, 129, 7106-7113.

"Double Lanthanide-Binding Tags for Macromolecular Crystallographic Structure Determination" Silvaggi, N. J.; Martin, L. J.; Schwalbe, H.; Imperiali, B.; Allen, K. N. J. Am. Chem. Soc. 2007, 129, 7114-7120.

"Tools for Investigating Peptide-Protein Interactions: Peptide Incorporation of Environment-Sensitive Fluorophores via on Resin Derivatization" Sainlos, M.; Imperiali, B. Nature Protocols 2007, 2, 3201-3209.

"Tools for Investigating Peptide-Protein Interactions: Peptide Incorporation of Environment-Sensitive Fluorophores through SPPS-based ‘Building Block’ Approach" Sainlos, M.; Imperiali, B. Nature Protocols, 2007, 2, 3210-3218.

"Synthesis of Anhydride Precursors of the Environment-Sensitive Fluorophores 4-DMAP and 6-DMN" Sainlos, M.; Imperiali, B. Nature Protocols, 2007, 2, 3219-3225.

"Polyisoprene Specificity in the Campylobacter jejuni N-linked Glycosylation Pathway" Chen, M. M; Weerapana, E.; Ciepichal, E.; Stupak, J.; Reid, C. W.; Swiezewska, E.; Imperiali, B. Biochemistry, 2007, 46, 14342-14348. PMC2585822

2006

"Asparagine-linked Protein Glycosylation: From Eukaryotic to Prokaryotic Systems" Weerapana, E.; Imperiali, B. Glycobiology, 2006, 16, 91R-101R.

"Optimal Sox-based Fluorescence Chemosensor Design for Serine/Threonine Protein Kinases" Shults, M. D.; Carrico-Moniz, D.; Imperiali, B. Analytical Biochem. 2006, 352, 198-207.

"Expression of N-Terminal Cys-Protein Fragments using an Intein Refolding Strategy" Hackenberger, C. P. R.; Chen, M. M.; Imperiali, B. Bioorg. Med. Chem. 2006, 14, 5043-5048.

"Direct Biochemical Evidence for the Utilization of UDP-Bacillosamine by PglC, an Essential Glycosyl-1-phosphate-transferase in the C. jejuni N-linked Glycosylation Pathway" Glover, K. J.; Weerapana, E.; Chen, M. M.; Imperiali, B. Biochemistry, 2006, 45, 5343-5350.

"Lanthanide Binding Tags as Luminescent Probes for Studying Protein Interactions" Sculimbrene, B. R.; Imperiali, B. J. Am. Chem. Soc. 2006, 128, 7346-7352.

"In vitro Evidence for the Dual Function of Alg2 and Alg11: Essential Mannosyltransferases in N-linked Glycoprotein Biosynthesis" O’Reilly, M. K.; Zhang, G.; Imperiali, B. Biochemistry, 2006, 45, 9593-9603.

"In vitro biosynthesis of UDP-N,N’-diacetylbacillosamine by Enzymes of the Campylobacter jejuni General Protein Glycosylation System" Olivier, N. B.; Chen, M. M.; Behr, J. R.; Imperiali, B. Biochemistry 2006, 45, 13659-13669. PMC2542654

2005

"Caged Phosphoproteins," Rothman, D. M.; Petersson, E. J.; Vázquez, M. E.; Brandt, G. S.; Dougherty, D. A.; Imperiali, B. J. Am. Chem. Soc. 2005, 127, 846-847.

"Photophysics and Biological Applications of the Environment-Sensitive Fluorophore 6-N,N-Dimethylamino-2,3-Naphthalimide" Vázquez, M. E.; Canosa, J. B. B.; Imperiali, B. J. Am. Chem. Soc. 2005, 127, 1300-1306.

"Design of a Heterospecific, Tetrameric, 21-Residue Miniprotein with Mixed α/β Structure" Ali, M. H.; Taylor, C. M.; Grigoryan, G.; Allen, K. N.; Imperiali, B.; Keating, A. E. Structure, 2005, 13, 225-234.

"A Multiplexed Homogenous Fluorescence-Based Assay for Protein Kinase Activity in Cell Lysates," Shults, M. D.; Janes, K. A.; Lauffenburger, D. A.; Imperiali, B. Nature Methods, 2005, 2, 277-284.

"Improving Glycopeptide Synthesis: A Convenient Protocol for the Preparation of β−Glycosylamines and the Synthesis of Glycopeptides" Hackenberger, C. P. R.; O’Reilly, M. O.; Imperiali, B. J. Org. Chem. 2005, 70, 3574-3578.

"The Engineering of Membrane-Permeable Peptides" Carrigan, C. N.; Imperiali, B., Analytical Biochem. 2005, 341, 290-298.

"In situ Photoactivation of a Caged Phosphotyrosine Peptide Derived from Focal Adhesion Kinase Temporarily Halts Lamellar Extension of Single Migrating Tumor Cells" David Humphrey, D.; Rajfur, Z.; Vazquez, M. E.; Scheswohl, D.; Schaller, M. D.; Jacobson, K.; Imperiali, B. J. Biol. Chem. 2005, 280, 22091-22101.

"Protein Oligomerization: How and Why" Ali, M. H.; Imperiali, B. Bioorg. Med. Chem. 2005, 13, 5013-5020.

"Chemical Approaches for Investigating Phosphorylation in Signal Transduction Networks" Rothman, D. M.; Shults, M. D.; Imperiali, B. Trends Cell. Biol. 2005, 15, 502-510.

"Semi-synthesis of a Glycosylated Im7 Analogue for Protein Folding Studies" Hackenberger, C. P. R; Friel, C. T.; Radford, S. E.; Imperiali, B. J. Am. Chem. Soc. 2005, 127, 12882-12889. PMC1356972

"In vitro Assembly of the Undecaprenylpyrophosphate-Linked Heptasaccharide for Prokaryotic N-linked Glycosylation" Glover, K. J.; Weerapana, E.; Imperiali, B. Proc. Natl. Acad. Sci. USA, 2005, 102, 14255-14259. PMC1242339

"Investigating Bacterial N-linked Glycosylation: Synthesis and Glycosyl Acceptor Activity of Undecaprenylpyrophosphate-linked Bacillosamine" Weerapana, E.; Glover, K. J.; Chen, M. M.; Imperiali, B. J. Am. Chem. Soc. 2005, 127, 13766-13767. PMC1351108

"Rapid Combinatorial Screening of Peptide Libraries for the Selection of Lanthanide-Binding Tags (LBTs)" Martin, L. J.; Sculimbrene, B. R.; Nitz, M.; Imperiali, B. QSAR Comb. Sci. 2005, 10, 1149-1157.

"Chemoenzymatic Synthesis of Glycopeptides using PglB, a Bacterial Oligosaccharyl Transferase from Campylobacter jejuni " Glover, K. J.; Weerapana, E.; Imperiali, B. Chemistry & Biology 2005, 12, 1311-1316. PMC2519243

"Application of Photoactivation and CALI (Chromophore-Assisted Light Inactivation) Technologies to Problems in Cell Motility," Humphrey, D.; Rajfur, Z.; Imperiali, B.; Marriott, G.; Roy, P.; Jacobson, K. in Live Cell Imaging: A Laboratory Manual, Spector, D. L.; Goldman, R. D. Eds. Cold Spring Harbor Laboratory Press (2005), Chapter 10, pp 159-176.

2004

"Structural Origin of the High Affinity of a Chemically Evolved Lanthanide Binding Peptide," Nitz, M.; Sherawat, M.; Franz, K. J.; Peisach, E.; Allen, K. N.; Imperiali, B. Angew. Chem. Int. Ed. 2004, 43, 3682-3685.

"Effects of Glycosylation on Peptide Conformation: A Synergistic Experimental and Computational Study," Bosques, C. J.; Tschampel, S. M.; Woods, R. J.; Imperiali, B. J. Am. Chem. Soc. 2004, 126, 8421-8425. PMC1386730

"Caged Phosphopeptides Reveal a Temporal Role for 14-3-3 in G1 Arrest and S-Phase Checkpoint Function," Nguyen, A.; Rothman, D. M.; Stehn, J.; Imperiali, B.; Yaffe, M. B. Nature Biotechnology, 2004, 22, 993-1000.

"X-Ray Structure Analysis of a Designed Oligomeric Mini-Protein Reveals a Discrete Quaternary Architecture," Ali, M. H.; Peisach, E.; Allen, K. N.; Imperiali, B. Proc. Natl Acad. Sci. USA, 2004, 101, 12183-12188. PMC514454

"A New Environment-Sensitive Fluorescent Amino Acid for Fmoc-based Solid Phase Peptide Synthesis," Vázquez, M. E.; Rothman, D. M.; Imperiali, B. Org. Biomol. Chem. 2004, 2, 1965-1966.

"Heterologous Expression and Biophysical Characterization of Soluble Oligosaccharyl Transferase Subunits," Dempski, R. E. Jr.; Imperiali, B. Arch. Biochem. Biophys. 2004, 431, 63-70.

2003

"Peptides to Peptidomimetics: Towards the Design and Synthesis of Bioavailable Inhibitors of Oligosaccharyl Transferase," Weerapana, E.; Imperiali, B. Org. Biomol. Chem. 2003, 1, 93-99.

"Lanthanide-Binding Tags as Versatile Protein Coexpression Probes," Franz, K. J.; Nitz, M.; Imperiali, B. ChemBioChem. 2003, 4, 265-271.

"A Powerful Combinatorial Screen to Identify High-Affinity Terbium(III)-Binding Peptides," Nitz, M.; Franz, K. J.; Maglathlin, R. L.; Imperiali, B. ChemBioChem. 2003, 4, 272-276.

"A Chemist’s Approach to Biochemical Complexity,” Aldridge, S.; Imperiali, B. Chem. Comm. 2003, 445-447.

"The Interplay of Glycosylation and Disulfide Formation Influences Fibrillization in a Prion Protein Fragment," Bosques, C. J.; Imperiali, B. Proc. Natl. Acad. Sci. USA 2003, 100, 7593-7598. PMC164631

"Chemistry and Biochemistry of Asparagine-Linked Protein Glycosylation," Imperiali, B.; Tai, V. W-F. in "Carbohydrate-based Drug Discovery"; Wong, C. H., ed. Wiley-VCH Verlag GmbH Publisher: Weinheim, 2003, pp 281-303.

"Photolytic Control of Peptide Self-Assembly," Bosques, C. J.; Imperiali, B. J. Am. Chem. Soc. 2003, 125, 7530-7531.

"Fluorescent Caged Phosphoserine Peptides as Probes to Investigate Phosphate-Dependent Protein Associations," Vázquez, M. E.; Nitz, M.; Stehn, J.; Yaffe, M. B. Imperiali, B. J. Am. Chem. Soc. 2003, 125, 10150-10151.

"A Modular and Tunable Chemosensor Scaffold for Divalent Zinc," Shults, M. D.; Pearce, D. A.; Imperiali, B. J. Am. Chem. Soc. 2003, 125, 10591-10597.

"Caged Phospho-Amino Acid Building Blocks Amenable to Fmoc-based Solid Phase Peptide Synthesis," Rothman, D. M.; Vázquez, M. E.; Vogel, E. M.; Imperiali, B. J. Org. Chem. 2003, 68, 6795-6798.

"Protein Alignment by a Coexpressed Lanthanide-Binding Tag for the Measurement of Residual Dipolar Couplings," Wöhnert, J.; Franz, K. J.; Nitz, M.; Imperiali, B.; Schwalbe, H. S. J. Am. Chem. Soc. 2003, 125, 13338-13339.

"Versatile Fluorescence Probes of Protein Kinase Activity," Shults, M. D.; Imperiali, B. J. Am. Chem. Soc. 2003, 125, 14248-14249.

2002

"Oligomeric ββα Mini-Protein Motifs: Pivotal Role of Single Hinge Residue in Determining the Oligomeric State," McDonnell, K. A.; Imperiali, B. J. Am. Chem. Soc. 2002, 124, 428-433.

"A General Method for the Synthesis of Caged Phosphopeptides: Tools for the Exploration of Signal Transduction Pathways," Rothman, D. M.; Vázquez, M. E.; Vogel, E. M.; Imperiali, B. Org. Lett. 2002, 4, 2865-2868.

"Enantioselective Synthesis and Application of the Highly Fluorescent and Environment-Sensitive Amino Acid 6-(2-dimethylaminonaphthoyl) Alanine (DANA)," Nitz, M.; Mezo, A. R.; Ali, M. H.; Imperiali, B. Chem. Comm. 2002, 1912-1913.

"Neoglycopeptides as Inhibitors of Oligosaccharyl Transferase: Insight into Negotiating Product Inhibition," Peluso, S.; Ufret, M. de L.; O'Reilly, M. K.; Imperiali, B. Chemistry & Biology 2002, 9, 1323-1328.

"Oligosaccharyl Transferase: Gatekeeper to the Secretory Pathway," Dempski, R. E. Jr.; Imperiali, B. Curr. Op. Chem. Biol. 2002, 6, 844-850.

2001

"Design of a Discretely Folded Mini-Protein Motif with Predominantly β-Structure," Ottesen, J. J.; Imperiali, B. Nature Structural Biology, 2001, 8, 535-539.

"Substrate Specificity of N-Acetylglucosaminyl(diphosphodolichol) N-Acetylglucosaminyl Transferase, a Key Enzyme in the Dolichol Pathway," Tai, V. W.-F.; O’Reilly, M. K.; Imperiali, B. Bioorg. Med. Chem. 2001, 9, 1133-1140.

“Discovery and Characterization of a Discretely Folded Homotrimeric ββα Peptide,” Mezo, A. R.; Ottesen, J. J.; Imperiali, B. J. Am. Chem. Soc. 2001, 123, 1002-1003.

"Derivatives of 8-Hydroxy-2-methyl-quinoline are Powerful Prototypes for Zinc Sensors in Biological Systems," Pearce, D. P.; Jotterand, N.; Carrico, I. S.; Imperiali, B. J. Am. Chem. Soc. 2001, 123, 5160-5161.

"Asymmetric Synthesis of a New 8-Hydroxyquinoline Derived α-Amino Acid and its Incorporation in a Peptidyl Sensor for Divalent Zinc," Jotterand, N.; Pearce, D. A.; Imperiali, B. J. Org. Chem. 2001, 66, 3224-3228.

"Oligomerization of Uniquely Folded Mini-Protein Motifs: Development of a Homotrimeric ββα Peptide," Mezo, A. R.; Cheng, R. P.; Imperiali, B. J. Am. Chem. Soc. 2001, 123, 3885-3891.

"Asparagine Surrogates for the Assembly of N-linked Glycopeptide Mimetics by Chemoselective Ligation," Peluso, S.; Imperiali, B. Tetrahedron Lett. 2001, 42, 2085-2087.

"Probing the Effect of the Outer Saccharide Residues of N-Linked Glycans on Peptide Conformation," O'Connor, S. E.; Pohlmann, J.; Imperiali, B.; Saskiawan, I.; Yamamoto, K. J. Am. Chem. Soc. 2001, 123, 6187-6188.

"Substrate Specificity for the Glycosyl Donor of Oligosaccharyl Transferase," Tai, V. W.-F.; Imperiali, B. J. Org. Chem. 2001, 66, 6217-6228.

"Stereoselective Synthesis of β−Linked TBDMS-Protected Chitobiose-Asparagine: A Versatile Building Block for Amyloidogenic Glycopeptides," Bosques, C. J.; Tai, V. W.-F.; Imperiali, B. Tetrahedron Lett. 2001, 42, 7207-7210.