SELECTED PUBLICATIONS

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Many of these articles are available for download in PDF format.
Adalsteinsson VA, Tahirova N, Tallapragada N, Yao X, Campion L, Angelini A, Douce TB, Huang C, Bowman B, Williamson CA, Kwon DS, Wittrup KD, Love JC. Single cells from human primary colorectal tumors exhibit polyfunctional heterogeneity in secretions of ELR+ CXC chemokines. Integr Biol (Camb). 2013 Oct;5(10):1272-81. [Pubmed]
Pirie CM, Liu DV, Wittrup KD. Targeted cytolysins synergistically potentiate cytoplasmic delivery of gelonin immunotoxin. Mol Cancer Ther. 2013 Sep;12(9):1774-82. [Pubmed]
Yao X, Labelle M, Lamb CR, Dugan JM, Williamson CA, Spencer DR, Christ KR, Keating RO, Lee WD, Paradis GA, Begum S, Hynes RO, Wittrup KD. Determination of 35 cell surface antigen levels in malignant pleural effusions identifies CD24 as a marker of disseminated tumor cells. Int J Cancer. 2013 Dec 15;133(12):2925-33. [Pubmed]
Su X, Yang N, Wittrup KD, Irvine DJ. Synergistic Antitumor Activity from Two-Stage Delivery of Targeted Toxins and Endosome-Disrupting Nanoparticles. Biomacromolecules. 2013 Apr 8;14(4):1093-102. [Pubmed]
Kwong B, Gai SA, Elkhader J, Wittrup KD, Irvine DJ. Localized Immunotherapy via Liposome-Anchored Anti-CD137 + IL-2 Prevents Lethal Toxicity and Elicits Local and Systemic Antitumor Immunity. Cancer Res. 2013 Mar 1;73(5):1547-58. [Pubmed]
Chen TF, de Picciotto S, Hackel BJ, Wittrup KD. Engineering fibronectin-based binding proteins by yeast surface display. Methods Enzymol. 2013;523:303-26. [Pubmed]
Yazaki PJ, Lee B, Channappa D, Cheung CW, Crow D, Chea J, Poku E, Li L, Andersen JT, Sandlie I, Orcutt KD, Wittrup KD, Shively JE, Raubitschek A, Colcher D. A series of anti-CEA/anti-DOTA bispecific antibody formats evaluated for pre-targeting: comparison of tumor uptake and blood clearance. Protein Eng Des Sel. 2013 Mar;26(3):187-93. Epub 2012 Nov 21. [Pubmed]
Mata-Fink J, Kriegsman B, Xin YH, Zhu H, Hanson M, Irvine DJ, Wittrup D. Rapid Conformational Epitope Mapping of anti-gp120 Antibodies with a Designed Mutant Panel Displayed on Yeast. J Mol Biol. 2013 Jan 23;425(2):444-56. Epub 2012 Nov 15. [Pubmed]
Thurber GM, Dane Wittrup K. A mechanistic compartmental model for total antibody uptake in tumors. J Theor Biol. 2012 Dec 7;314:57-68. [Pubmed]
Bak SP, Barnkob MS, Bai A, Higham EM, Wittrup KD, Chen J. Differential requirement for CD70 and CD80/CD86 in dendritic cell-mediated activation of tumor-tolerized CD8 T cells. J Immunol. 2012 Aug 15;189(4):1708-16. [Pubmed]
Spangler JB, Manzari MT, Rosalia EK, Chen TF, Wittrup KD. Triepitopic antibody fusions inhibit cetuximab-resistant BRAF- and KRAS-mutant tumors via EGFR signal repression. J Mol Biol. 2012 Sep 28;422(4):532-44. [Pubmed]
Orcutt KD, Rhoden JJ, Ruiz-Yi B, Frangioni JV, Wittrup KD. Effect of small-molecule-binding affinity on tumor uptake in vivo: a systematic study using a pretargeted bispecific antibody. Mol Cancer Ther. 2012 Jun;11(6):1365-72. Epub 2012 Apr 5. [Pubmed]

Wittrup KD, Verdine GL, ed., Protein engineering for therapeutics, Part B. Methods Enzymol. 2012;503 [ScienceDirect]

Wittrup KD, Verdine GL, ed., Protein engineering for therapeutics, Part A. Methods Enzymol. 2012;502 [ScienceDirect]

Wittrup KD, Thurber GM, Schmidt MM, Rhoden JJ. Practical theoretic guidance for the design of tumor-targeting agents. Methods Enzymol. 2012;503:255-68. [Pubmed]
Hackel BJ, Neil JR, White FM, Wittrup KD. Epidermal growth factor receptor downregulation by small heterodimeric binding proteins. Protein Eng Des Sel. 2011 Dec 9. [Epub ahead of print] [Pubmed]
Rhoden JJ, Wittrup KD. Dose dependence of intratumoral perivascular distribution of monoclonal antibodies. J Pharm Sci. 2012 Feb;101(2):860-7. Epub 2011 Nov 4.[Pubmed]
Boersma YL, Chao G, Steiner D, Wittrup KD, Plückthun A. Bispecific Designed Ankyrin Repeat Proteins (DARPins) Targeting Epidermal Growth Factor Receptor Inhibit A431 Cell Proliferation and Receptor Recycling. J Biol Chem. 2011 Dec 2;286(48):41273-85. Epub 2011 Oct 6. [Pubmed]
Schiefner A, Chatwell L, Körner J, Neumaier I, Colby DW, Volkmer R, Wittrup KD, Skerra A. A Disulfide-Free Single-Domain V(L) Intrabody with Blocking Activity towards Huntingtin Reveals a Novel Mode of Epitope Recognition.J Mol Biol. 2011 Dec 2;414(3):337-55. Epub 2011 Sep 24. [Pubmed]
Ackerman ME, Lai JI, Pastan I, Wittrup KD. Exploiting bias in a non-immune human antibody library to predict antigenicity. Protein Eng Des Sel. 2011 Nov;24(11):845-53. Epub 2011 Sep 9. [Pubmed]
Orcutt KD, Slusarczyk AL, Cieslewicz M, Ruiz-Yi B, Bhushan KR, Frangioni JV, Wittrup KD. Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging. Nucl Med Biol. 2011 Feb;38(2):223-33. Epub 2010 Oct 27. [Pubmed]
Pirie CM, Hackel BJ, Rosenblum MG, Wittrup KD. Convergent potency of internalized gelonin immunotoxins across varied cell lines, antigens, and targeting moieties. J Biol Chem. 2011 Feb 11;286(6):4165-72. Epub 2010 Dec 7. [Pubmed]
Spangler JB, Neil JR, Abramovitch S, Yarden Y, White FM, Lauffenburger DA, Wittrup KD. Combination antibody treatment down-regulates epidermal growth factor receptor by inhibiting endosomal recycling. Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13252-7. Epub 2010 Jul 7. [Pubmed]
Hackel BJ, Ackerman ME, Howland SW, Wittrup KD. Stability and CDR composition biases enrich binder functionality landscapes. J Mol Biol. 2010 Aug 6;401(1):84-96. Epub 2010 Jun 9. [Pubmed]
Orcutt KD, Nasr KA, Whitehead DG, Frangioni JV, Wittrup KD. Biodistribution and Clearance of Small Molecule Hapten Chelates for Pretargeted Radioimmunotherapy. Mol Imaging Biol. 2010 Jun 9. [Pubmed]
Higham EM, Shen CH, Wittrup KD, Chen J. Cutting edge: delay and reversal of T cell tolerance by intratumoral injection of antigen-loaded dendritic cells in an autochthonous tumor model. J Immunol. 2010 Jun 1;184(11):5954-8. Epub 2010 Apr 28. [Pubmed]
Higham EM, Wittrup KD, Chen J. Activation of tolerogenic dendritic cells in the tumor draining lymph nodes by CD8+ T cells engineered to express CD40 ligand. J Immunol. 2010 Apr 1;184(7):3394-400. Epub 2010 Mar 3. [Pubmed]
Hackel BJ, Wittrup KD. The full amino acid repertoire is superior to serine/tyrosine for selection of high affinity immunoglobulin G binders from the fibronectin scaffold. Protein Eng Des Sel. 2010 Apr;23(4):211-9. Epub 2010 Jan 12. [Pubmed]
Orcutt KD, Ackerman ME. Ciesclewicz M, Quiroz E, Slusarcyz AL, Frangioni JV, Wittrup KD. A modular IgG-scFv bispecific antibody topology. PEDS. 2009. [Pubmed]
Schmidt MM, Wittrup KD. A modeling analysis of the effects of molecular size and binding affinity on tumor targeting. Mol Cancer Ther. 8(10):2861-71, 2009. [Pubmed]
Liu DV, Maier LM, Hafler DA, Wittrup KD. Engineered interleukin-2 antagonists for the inhibition of regulatory T cells. J Immunother. 2009. [Pubmed]
Tasumi S, Velikovsky CA, Xu G, Gai SA, Wittrup KD, Flajnik MF, Mariuzza RA, Pancer Z. High-affinity lamprety VLRA and VLRB monoclonal antibodies. PNAS. 106(31):12891-6, 2009. [Pubmed , Journal]
Rakestraw JA, Sazinsky SL, Piatesi A, Antipov E, Wittrup KD. Directed evolution of a secretory leader for the improved expression of heterologous proteins and full-length antibodies in Saccharomyces cerevisiae. Biotechnol Bioeng. 103(6):1192-201, 2009. [PubMed , Journal]
Ackerman M, Levary D, Tobon G, Hackel B, Orcutt KD, Wittrup KD. Highly avid magnetic bead capture: An efficient selection method for de nova protein engineering utilizing yeast surface display. Biotechnol Prog. 25(3):774-83, 2009. [PubMed]
Garrett TP, Burgess AW, Gan HK, Luwor RB, Cartwright G, Walker F, Orchard SG, Clayton AH, Nice EC, Rothacker J, Catimel B, Cavenee WK, Old LJ, Stockert E, Ritter G, Adams TE, Hoyne PA, Wittrup D, Chao G, Cochran JR, Luo C, Lou M, Huyton T, Xu Y, Fairlie WD, Yao S, Scott AM, Johns TG. Antibodies specifically targeting a locally misfolded region of tumor associated EGFR. PNAS. 106(13):5082-7, 2009. [PubMed , Journal]
Maier LM , Anderson DE, Severson CA, Baecher-Allan C, Healy B, Liu DV, Wittup KD, De Jager PL, Hafler DA. Soluble IL-2RA levels in multiple sclerosis subjects and the effect of soluble IL-2RA on immune responses. J Immunol. 182(3):1541-7, 2009. [PubMed , Journal]
Sazinsky SL, Ott RG, Silver NW, Tidor B, Ravetch JV, Wittrup KD. Aglycosylated immunoglobulin G1 variants productively engage activating Fc receptors. PNAS. 105:20167-72, 2008. [PubMed , Journal]
Antipov E, Cho AE, Wittrup KD, Klibanov AM. Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method. PNAS. 105(46):17694-9, 2008. [PubMed , Journal]
Bai A, Higham E, Eisen HN, Wittrup KD, Chen J. Rapid tolerization of virus-activated tumor-specific CD8+ T cells in prostate tumors of TRAMP mice. PNAS. 105(35): 13003-8, 2008. [PubMed , Journal]
Hackel BJ, Kapila A, Wittrup KD. Picomolar affinity fibronectin domains engineered utilizing loop length diversity, recursive mutagenesis, and loop shuffling. J Mol Biol. 381(5):159-65, 2008. [PubMed , Journal]
Ackerman ME, Pawlowski D, Wittrup KD. Effect of antigen turnover rate and expression level on antibody penetration into tumor spheroids. Mol Cancer Ther. 7(7):2233-40, 2008. [PubMed , Journal]
Howland SW, Tsuji T, Gnjatic S, Ritter G, Old LJ, Wittrup KD. Inducing efficient cross-priming using antigen-coated yeast particles. J Immunother. 31(7):607-619, 2008. [PubMed]
Thurber GM, Schmidt MM, Wittrup KD. Antibody tumor penetration: Transport opposed by systemic and antigen-mediated clearance. Adv Drug Deliv Rev. 60(12):1421-34, 2008. [PubMed , Journal]
Thurber GM, Wittrup KD. Quantitative spatiotemporal analysis of antibody fragment diffusion and endocytic consumption in tumor spheroids. Cancer Res. 68(9):3334-41, 2008. [PubMed , Journal]
Howarth M, Liu W, Puthenveetil S, Zheng Y, Marshall LF, Schmidt MM, Wittrup KD, Bawendi MG, Ting AY. Monovalent, reduced-size quantum dots for imaging receptors on living cells. Nat Methods, 5(5):397-9, 2008. [PubMed , Journal]
Schmidt MM, Thurber GM, Wittrup KD. Kinetics of anti-carcinoembryonic antigen antibody internalization: effects of affinity, bivalency, and stability. Cancer Immunol Immunother. 2008. [PubMed , Journal]
Yeung YA, Finney AH, Koyrakh IA, Lebowitz MS, Chanbari HA, Wands JR, Wittrup KD. Isolation and characterization of human antibodies targeting human aspartyl (asparaginyl) beta-hydroxylase. Hum Antibodies, 16(3-4):163-176, 2007. [PubMed , Journal]
Ackerman ME, Chalouni C, Schmidt MM, Raman VV, Ritter G, Old LJ, Mellman I, Wittrup KD. A33 antigen displays persistent surface expression. Cancer Immunol Immunother. 57(7):1017-1027, 2008. [PubMed , Journal]
Howland SW, Wittrup KD. Antigen release kinetics in the phagosome are critical to cross-presentation efficiency. J Immunol. 180(3):1576-83, 2008. [PubMed , Journal]
Thurber GM, Schmidt MM, Wittrup KD. Factors determining antibody distribution in tumors. Trends Pharmacol Sci. 29(2):57-61, 2008. [PubMed , Journal]
Lipovsek D, Antipov E, Armstrong KA, Olsen MJ, Klibanov AM, Tidor B, Wittrup KD. Selection of horseradish peroxidase variants with enhanced enantioselectivity by yeast surface display. Chem Biol. 14(10):1176-85, 2007. [PubMed , Journal]
Lippow SM, Wittrup KD, Tidor B. Computational design of antibody-affinity improvement beyond in vivo maturation. Nat Biotechnol. 25(10):1171-76, 2007. [PubMed , Journal]
Gai SA, Wittrup KD. Yeast surface display for protein engineering and characterization. Curr Opin Struct Biol. 17(4):467-73, 2007. [PubMed , Journal]
Thurber GM, Zajic SC, Wittrup KD. Theoretic Criteria for Antibody Penetration into Solid Tumors and Micrometastases. J Nucl Med. 48(6):995-9, 2007. [PubMed , Journal]
Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, Wittrup KD. Isolating and engineering human antibodies using yeast surface display. Nat Protoc. 1(2): 755-768, 2007. [Pubmed , Journal]
Lipovsek D, Lippow SM, Hackel BJ, Gregson MW, Cheng P, Kapila A, Wittrup KD. Evolution of an Interloop Disulfide Bond in High-Affinity Antibody Mimics Based on Fibronectin Type III Domain and Selected by Yeast Surface Display: Molecular Convergence with Single-Domain Camelid and Shark Antibodies. J. Mol. Biol. 368(4):1024-41, 2007. [PubMed , Journal]
Krauland EM, Peele BR, Wittrup KD, Belcher AM. Peptide tags for enhanced cellular and protein adhesion to single-crystalline sapphire. Biotechnol Bioeng. 97(5):1009-20, 2007. [PubMed , Journal]
Swers JS, Widom A, Phan U, Springer TA, Wittrup KD. A high affinity human antibody antagonist of P-selectin mediated rolling. Biochem Biophys Res Commun. 350(3): 508-513, 2006. [PubMed, Journal]
Jin M, Song G, Carman CV, Kim YS, Astrof NS, Shimaoka M, Wittrup DK, Springer TA. Directed evolution to probe protein allostery and integrin I domains of 200,000-fold higher affinity. Proc Natl Acad Sci U S A. 103(15): 5758-5763, 2006. [PubMed , Journal]
Sivasubramanian A, Chao G, Pressler HM, Wittrup KD, Gray JJ. Structural Model of the mAb 806-EGFR Complex Using Computational Docking followed by Computational and Experimental Mutagenesis. Structure 14(3):401-14, 2006. [PubMed, Journal]
J.A. Rakestraw, A. R. Baskaran, K.D. Wittrup. A Flow Cytometric Assay for Screening Improved Heterologous Protein Secretion in Yeast. Biotechnol Prog. 22(4): 1200-1208, 2006. [PubMed, Journal]
Piatesi A, Howland SW, Rakestraw JA, Renner C, Robson N, Cebon J, Maraskovsky E, Ritter G, Old L, and Wittrup KD. Directed Evolution for Improved Secretion of Cancer-Testis Antigen NY-ESO-1 from Yeast. Protein Expr. Purif. 48(2): 232-242, 2006. [PubMed , Journal]
Rakestraw A, Wittrup KD. Contrasting secretory processing of simultaneously expressed heterologous proteins in Saccharomyces cerevisiae. Biotechnol Bioeng. 93 (5): 896-905, 2006. [PubMed, Journal]
Cochran J.R., Rao, B.M., Kim, Y.-S., Wittrup, K.D. Improved Mutants from Directed Evolution are Biased to Orthologous Substitutions. Protein Engineering, Design, & Selection, 19(6): 245-253, 2006. [PubMed, Journal]
Colby, D.W., Cassady, J., Lin, G., Ingram, V.M., Wittrup, K.D. A stochastic model of intracellular aggregate formation in Huntington's disease. Nature Chemical Biology, 2(6): 319-323, 2006. [PubMed, Journal]
Midelfort, K.S., Wittrup, K.D. Context-Dependent Mutations Predominate in an Engineered High Affinity Single Chain Antibody Fragment. Protein Science, 15(2):324-34, 2006. [PubMed, Journal]
Kim Y.S., Bhandari R., Cochran J.R., Kuriyan J., Wittrup K.D. Directed Evolution of the Epidermal Growth Factor Receptor Extracellular Domain for Expression in Yeast. Proteins: Structure, Function, & Bioinformatics, 62(4):1026-35, 2006. [PubMed, Journal]
Rao BM, Driver I, Lauffenburger DA, Wittrup KD. High-affinity CD25-binding IL-2 mutants potently stimulate persistent T cell growth. Biochemistry. 44(31):10696-701, 2005. [PubMed, Journal]
Peelle BR, Krauland EM, Wittrup KD, Belcher AM Design criteria for engineering inorganic material-specific peptides. Langmuir. 2005 Jul 19;21(15):6929-33. [PubMed, Journal]
Peelle, B.R., Krauland, E., Wittrup, K.D., Belcher, A.M. Probing the interface between biomolecules and inorganic materials using yeast surface display and genetic engineering. Acta Biomaterialia 1(2):145-154, 2005. [PubMed, Journal]
Rao, BM, Lauffenburger, DA, Wittrup KD. Integrating cell-level kinetic modeling into the design of engineered protein therapeutics. Nature Biotechnology, 23(2):191-4, 2005. [PubMed, Journal]
Colby DW, Chu Y, Cassady JP, Duennwald M, Zazulak H, Webster JM, Messer A, Lindquist S, Ingram VM, Wittrup KD. Potent inhibition of huntingtin aggregation and cytotoxicity by a disulfide bond-free single-domain intracellular antibody. Proc Natl Acad Sci U S A. 101(51): 17616-17621, 2004. [PubMed, Journal]
Mohr L, Yeung A, Aloman C, Wittrup D, Wands JR. Antibody-directed therapy for human hepatocellular carcinoma. Gastroenterology. 127(5 Suppl 2):S225-31, 2004.
Midelfort, K.S., Hernandez, H.H., Lippow, S.M., Tidor, B., Drennan, C.L., Wittrup, K.D. "Substantial Energetic Improvement with Minimal Structural Perturbation in a High Affinity Mutant Antibody." J. Mol. Biol., 343(3):685-701. [PubMed, Journal]
Rao, B.M., Driver, I., Lauffenburger D.A., Wittrup K.D. "IL-2 variants engineered for increased IL 2R-alpha affinity exhibit increased potency arising from a cell surface ligand reservoir effect." Molecular Pharmacology 66(4):864-869, 2004. [PubMed, Journal]
Chao, G., Cochran, J.R., Wittrup, K.D. "Fine Epitope Mapping of anti-Epidermal Growth Factor Receptor Antibodies through Random Mutagenesis and Yeast Surface Display." J. Mol. Biol. 342(2):539-50, 2004. [PubMed, Journal]
Colby, D.W., Garg, P., Holden, T., Chao, G., Webster, J.M., Messer, A., Ingram, V.M., Wittrup, K.D. "Development of a Human Light Chain Variable Domain (VL) Intracellular Antibody Specific for the Amino Terminus of Huntingtin via Yeast Surface Display." J. Mol. Biol. 342(3):901-12, 2004. [PubMed, Journal]
Johns TG, Adams TE, Cochran JR, Hall NE, Hoyne PA, Olsen MJ, Kim YS, Rothacker J, Nice EC, Walker F, Ritter G, Jungbluth AA, Old LJ, Ward CW, Burgess AW, Wittrup KD, Scott AM. "Identification of the Epitope for the Epidermal Growth Factor Receptor-specific Monoclonal Antibody 806 Reveals That It Preferentially Recognizes an Untethered Form of the Receptor." J. Biol Chem. 279(29):30375-30384, 2004. [PubMed, Journal]
Coughlan, C.M., Walker J.L., Cochran J.C., Wittrup K.D., Brodsky, J.L. "Degradation of mutated bovine pancreatic trypsin inhibitor (BPTI) in the yeast vacuole suggests post-endoplasmic reticulum protein quality control." J. Biol. Chem. 279(15):15289-97, 2004. [PubMed, Journal]
Graff, C.P., Chester K., Begent R., Wittrup, K.D. "Directed Evolution of an Anti-Carcinoembryonic Antigen scFv with a Four-Day Monovalent Dissociation Half-time at 37 ¡C." Protein Engineering, Design & Selection, 17:293, 2004. [PubMed, Journal]
Cochran, J.R., Kim, Y.S., Olsen, M.J., Bhandari, R., Wittrup, K.D. "Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments." J. Immunol. Methods, 287(1-2):147-158, 2003. [PubMed, Journal]
Colby, D.W., Kellog, B.A., Graff, C.P., Yeung, Y.A., Swers, J.S., Wittrup, K.D. "Engineering Antibody Affinity by Yeast Surface Display." Methods in Enzymology, 388:348, 2004. [PubMed]
Swers, J.S., Kellogg, B.A., Wittrup, K.D. "Shuffled Antibody Libraries Created by In Vivo Homologous Recombination and Yeast Surface Display." Nucleic Acids Research, 32(3):36, 2004. [PubMed, Journal]
Rao, B.M., Girvin, A.T., Ciardelli, T., Lauffenburger, D.A., Wittrup, K.D. "Interleukin-2 Mutants with Enhanced alpha-Receptor Subunit Binding Affinity." Protein Engineering, 16(12):1081-7, 2003. [PubMed, Journal]
Grovender EA, Kellogg B, Singh J, Blom D, Ploegh H, Wittrup KD, Langer RS, Ameer GA. "Single-chain antibody fragment-based adsorbent for the extracorporeal removal of beta2-microglobulin." Kidney Int. 65(1):310-322, 2004. [PubMed, Journal]
Bhatia SK, Swers JS, Camphausen RT, Wittrup KD, Hammer DA. "Rolling Adhesion Kinematics of Yeast Engineered To Express Selectins." Biotechnol Prog. 19(3):1033-1037, 2003. [PubMed, Journal]
Graff, C.P., Wittrup, K.D. "Theoretical Analysis of Antibody Targeting of Tumor Spheroids:Ê Importance of Dosage for Penetration, and Affinity for Retention." Cancer Research, 63:1288, 2003. [PubMed, Journal]
Feldhaus, M.J., Siegel, R.W., Opresko, L.K., Coleman, J.R., Weaver Feldhaus, J.M., Yeung, Y.A., Cochran, J.R., Heinzelman, P., Colby, D., Swers, J., Graff, C., Wiley, H.S., Wittrup, K.D., "Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library," Nature Biotechnology 21, 163-170, 2003. [PubMed, Journal]
Orr B.A., Carr L.M., Wittrup K.D., Roy E.J., Kranz D.M., "Rapid Method for Measuring ScFv Thermal Stability by Yeast Surface Display", Biotechnol Prog. 19(2):631-8 (2003). [PubMed]
Graff C.P., Wittrup K.D., "Theoretical analysis of antibody targeting of tumor spheroids: importance of dosage for penetration, and affinity for retention," Cancer Res. 63(6):1288-96 (2003). [PubMed]
Yeung Y.A., Wittrup K.D., "Quantitative screening of yeast surface-displayed polypeptide libraries by magnetic bead capture," Biotechnol Prog. 18(2):212-20 (2002). [PubMed]
Wittrup, K.D., "Protein Engineering by Cell-surface Display," Current Opinion in Biotechnology 12 (4) 395-399, 2001. [PubMed, Journal]
Kieke M.C., E. Sundberg, E.V. Shusta, R.A. Mariuzza, K.D. Wittrup, D.M. Kranz, "High Affinity T-cell Receptors from Yeast Display Libraries Block T-cell Activation by Superantigens," J Mol Biol 307(5), 1305-1315, 2001.  [PubMed, Journal]
Boder, E.T., K.D. Wittrup, "Yeast Surface Display for Directed Evolution of Protein Expression, Affinity, and Stability," Methods in Enzymology 328, Ed. J. Thorner and S.D. Emr, 430-444, 2000.  [PubMed, Journal]
Wittrup, K.D., "The Single Cell as a Microplate Well," Nature Biotechnolgy 18(10), 1039-1040, 2000.  [PubMed, Journal]
Boder, E.T., K.S. Midelfort , K.D. Wittrup, "Directed Evolution of Antibody Fragments with Monovalent Femtomolar Antigen-binding Affinity," Proc Natl Acad Sci USA, 97(20), 10701-10705, 2000.  [PubMed, Journal]
Shusta, E.V., P.D. Holler, M.C. Kieke, D.M. Kranz, K.D. Wittrup, "Directed Evolution of a Stable Scaffold for T-cell Receptor Engineering," Nature Biotechnology 18(7), 754-759, 2000.  [PubMed, Journal]
Holler P.D., P.O. Holman, E.V. Shusta, S. O'Herrin, K.D. Wittrup, D.M. Kranz, "In Vitro Evolution of a T cell receptor with High Affinity for peptide/MHC," Proc. Natl. Acad. Sci. USA, 97(10) 5387-5392, 2000. [PubMed, Journal]
Bannister, S.J.,  K.D. Wittrup, "Glutathione Excretion in Response to Heterologous Protein Secretion in Saccharomyces cerevisiae," Biotechnology and Bioengineering 68(4), 389-395, 2000.  [PubMed, Journal]
VanAntwerp, J.J., K.D. Wittrup, "Fine Affinity Discrimination by Yeast Surface Display and Flow Cytometry," Biotechnology Progress 16(1), 31-37, 2000.  [PubMed, Journal]
Woycechowsky, K.J., K.D. Wittrup, R.E. Raines, "A Small-molecule Catalyst of Protein Folding in vitroand in vivo,"  Chemistry and Biology 6(12), 871-879, 1999.  [PubMed, Journal]
Wittrup, K.D., "Phage On Display," Trends In Biotechnolgy 17(11), 423-424, 1999.  [PubMed, Journal]
F. Becker, L. Block-Alper, G. Nakamura, J. Harada, K.D. Wittrup, D.I. Meyer,  "Expression of the 180-kD Ribosome Receptor Induces Membrane Proliferation and Increased Secretory Activity in Yeast,"  J. Cell Biol. 146(2), 273-284, 1999.  [PubMed, Journal]
E.V. Shusta, M.C. Kieke, E. Parke, D.M. Kranz, K.D. Wittrup, "Yeast Polypeptide Fusion Surface Display Levels Predict Thermal Stability and Soluble Secretion Efficiency," J Mol Biol 292(5), 949-956, 1999. [PubMed, Journal]
Wittrup, K.D., "Heterologous Protein Secretion in Saccharomyces cerevisiae."  Encyclopedia of Bioprocess Engineering, Ed. M. Flickinger. [PubMed, Journal]
Wittrup, K. D., "Engineering Heterologous Secretion in Yeast,", Metabolic Engineering, Ed. E. T. Papoutsakis, in press.  [PubMed, Journal]
Kieke, M. C., E.V. Shusta, E. T. Boder, L. Teyton, K. D. Wittrup, D. M. Kranz, "Selection of Functional T Cell Receptor Mutants from a Yeast Surface-display Library," Proc. Natl. Acad. Sci. USA, 96(10), 5651-5656, 1999.  [PubMed, Journal]
VanAntwerp, J.J. and K. D. Wittrup, "Thermodynamic Characterization of Affinity Maturation:  The D1.3 Antibody and a Higher Affinity Mutant," J. Mol. Recog. 11(1-6), 10-13, 1998.  [PubMed, Journal]
Shusta, E. V., J.J. VanAntwerp, K. D. Wittrup, "Biosynthetic Polypeptide Libraries," Current Opinion in Biotechnology 10(2), 117-122, 1999. [PubMed, Journal]
Shusta, E. V., R. T. Raines, A. Plückthun, K. D. Wittrup, "Increasing the Secretory Capacity of Saccharomyces cerevisiae for Production of Single-chain Antibody Fragments," Nature Biotechnology 16(8), 773-777, 1998. [PubMed, Journal]
Kowalski, J. M., R. N. Parekh, J. Mao, and K. D. Wittrup, "Protein Folding Stability Can Determine the Efficiency of Escape from Endoplasmic Reticulum Quality Control," J. Biol. Chem. 273(31), 19453-19458, 1998. [PubMed, Journal]
Cho, B. K., M. C. Kieke, E. T. Boder, K. D. Wittrup and D. M. Kranz, "A Yeast Surface Display System for the Discovery of Ligands that Trigger Cell Activation," J. Immunol. Meth. 220(1-2), 179-188, 1998. [PubMed, Journal]
Kowalski, J. M., R. M. Kelly, J. Konisky, D. S. Clark and K. D. Wittrup, "Purification and Functional Characterization of a Chaperone from Methanococcus jannaschii," Systematic & Applied Microbiology 21(2), 173-178, 1998.  [PubMed, Journal]
Arnold, C. E., R. N. Parekh, W. Yang, and K. D. Wittrup, "Leader Peptide Efficiency Correlates with Signal Recognition Particle Dependence in Saccharomyces cerevisiae," Biotechnology & Bioengineering 59(3), 286-293, 1998.  [PubMed, Journal]
Boder, E. T. and K. D. Wittrup, "Optimal Screening of Surface-Displayed Polypeptide Libraries," Biotechnology Progress 14(1), 55-62, 1998. [PubMed, Journal]
Kowalski, J. M., R. N. Parekh, and K. D. Wittrup, "Secretion Efficiency in Saccharomyces cerevisiae of Bovine Pancreatic Trypsin Inhibitor Mutants Lacking Disulfide Bonds Is Correlated with Thermodynamic Stability," Biochemistry 37(5), 1264-1273, 1998.  [PubMed, Journal]
Kieke, M. C., B. K. Cho, E. T. Boder, D. M. Kranz, and K. D. Wittrup, "Isolation of Anti-T Cell Receptor scFv Mutants by Yeast Surface Display," Protein Engineering 10(11), 1303-1310, 1997.  [PubMed, Journal]
Boder, E. T. and K. D. Wittrup, "Yeast Surface Display for Screening Combinatorial Polypeptide Libraries," Nature Biotechnology 15(6), 553-537, 1997.  [PubMed, Journal]
Parekh, R. N. and K. D. Wittrup, "Expression Level Tuning for Optimal Heterologous Protein Secretion in Saccharomyces cerevisiae," Biotechnology Progress 13(2), 117-122, 1997.  [PubMed, Journal]
Robinson, A. S., J. A. Bockhaus, A. C. Voegler, and K. D. Wittrup, "Reduction of BiP Levels Decreases Heterologous Protein Secretion in Saccharomyces cerevisiae," J. Biol. Chem. 271(17), 10017, 1996.  [PubMed, Journal]
Parekh, R. N., M. R. Shaw, and K. D. Wittrup, "An Integrating Vector for Tunable, High Copy, Stable Integration into the Dispersed Ty-d  Sites of Saccharomyces cerevisiae," Biotechnology Progress 12(1), 16-21, 1996.  [PubMed, Journal]
Robinson, A. S. and K. D. Wittrup, "Constitutive Overexpression of Secreted Heterologous Proteins Decreases Extractable BiP and Protein Disulfide Isomerase Levels in Saccharomyces cerevisiae," Biotechnology Progress 11(2), 171-177, 1995.  [PubMed,  Journal]
Wittrup, K. D. "Disulfide Bond Formation and Eukaryotic Secretory Productivity," Current Opinion in Biotechnology 6(2), 203-208, 1995. [PubMed, Journal]
Parekh, R. N., K. Forrester, and K. D. Wittrup "Multicopy Overexpression of Bovine Pancreatic Trypsin Inhibitor Saturates the Protein Folding and Secretory Capacity of Saccharomyces cerevisiae," Protein Expression and Purification 6(4), 537-545, 1995.  [PubMed, Journal]
Arnold, C. E. and K. D. Wittrup "The Stress Response to Loss of Signal Recognition Particle Function in Saccharomyces cerevisiae," J. Biol. Chem. 269(48), 30412-30418, 1994.  [PubMed, Journal]
Wittrup, K. D., A. S. Robinson, R. N. Parekh, and K. J. Forrester, "Existence of an Optimum Expression Level for Secretion of Foreign Proteins in Yeast," Annals of NY Academy of Sciences 745, 321-330, 1994. [PubMed,  Journal]
Robinson, A. S., V. Hines, and K. D. Wittrup, "Protein Disulfide Isomerase Overexpression Increases Secretion of Foreign Proteins in Saccharomyces cerevisiae,"  Bio/Technology (NY) 12(4), 381-384, 1994. [PubMed,  Journal]
Wittrup, K. D. and V. Benig, "Optimization of Amino Acid Supplements for Heterologous Protein Secretion in Saccharomyces cerevisiae,"  Biotechnology Techniques 8, 161-166, 1994.  [PubMed,  Journal]
Wittrup, K. D., R. J. Westerman, and R. Desai "Fluorescence Array Detector for Large Field Quantitative Fluorescence Cytometry," Cytometry 16(3), 206-213, 1994.  [PubMed,  Journal]
Shaw, M. R., R. Schiek, and K. D. Wittrup, "Stochastic Simulation of 2m Multicopy Plasmid Amplification in Yeast," Biotechnology Progress 9(2), 160-165, 1993.  [PubMed,  Journal]
Robinson, A. S. and K. D. Wittrup, "The Role of the Protein Folding Chaperone BiP in Secretion of Foreign Proteins in Eukaryotic Cells," ACS Symp. Series 526, 121-132, 1993.  [PubMed,  Journal]
Wittrup, K. D., A. L. Weber, and P. Tsai, "Microencapsulation Selection for Isolation of Yeast Mutants with Increased Secretion of Aspergillus awamori Glucoamylase," Biotechnology & Bioengineering 42, 351-356, 1993.  [PubMed,  Journal]
Wittrup, K. D. and J. E. Bailey, "Comments on a segregated model of recombinant cultures:  authors' reply," Biotechnology & Bioengineering 38, 1364-1365, 1991.  [PubMed,  Journal]
Meilhoc, E., K. D. Wittrup, and J. E. Bailey, "Influence of Dissolved Oxygen Concentration on Growth, Mitochondrial Function and Antibody Production of Hybridoma Cells in Batch Culture." Bioprocess Engineering 5, 263, 1990.  [PubMed,  Journal]
Wittrup, K. D., B. Ratzkin, A. Patel, and J. E. Bailey, "Propagation of an Amplifiable Recombinant Plasmid in Saccharomyces cerevisiae:  Flow Cytometry Studies and Segregated Modeling," Biotechnology & Bioengineering 35, 565-577, 1990.  [PubMed,  Journal]
Wittrup, K. D. and J. E. Bailey, "Mathematical Modeling of a Single-Cell Enzyme Assay in Saccharomyces cerevisiae Using a Fluorogenic Substrate," Biotechnology & Bioengineering 35, 525-532, 1990.  [PubMed,  Journal]
Budd, M. E., K. D. Wittrup, J. E. Bailey, and J. L. Campbell, "DNA polymerase I is required for premeiotic DNA replication and sporulation but not for X-ray repair in Saccharomyces cerevisiae," Mol Cell Biol. 9(2), 365-376, 1989.  [PubMed,  Journal]
Meilhoc, E., K. D. Wittrup, and J. E. Bailey, "Application of flow cytometric measurement of surface IgG in kinetic analysis of monoclonal antibody synthesis and secretion by murine hybridoma cells," J. Immunol. Meth. 121(2), 167-174, 1989.  [PubMed,  Journal]
Wittrup, K. D. and J. E. Bailey, "A Single-cell Assay of  b-galactosidase Activity in Saccharomyces cerevisiae Using a Fluorogenic Substrate," Cytometry 9(4), 394-404, 1988.  [PubMed,  Journal]
Wittrup, K. D. and J. E. Bailey, "A Mathematical Model of Recombinational Amplification of the 2m  Plasmid in the Yeast Saccharomyces cerevisiae," J. of Theor. Biol. 130(4), 481-492, 1988.  [PubMed,  Journal]
Wittrup, K. D., M. Mann, D. Fenton, L. Tsai, and J. E. Bailey, "Single-cell Light Scatter as a Probe of Refractile Body Formation in Recombinant E. coli," Bio/Technology (NY) 6, 423-427, 1988.  [PubMed,  Journal]
Wittrup, K. D. and J. E. Bailey,  "A Segregated Model of Recombinant Multicopy Plasmid Propagation," Biotechnology & Bioengineering 31, 304-310, 1988.  [PubMed,  Journal]
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