• Services
  • Sequence of pF2L2
  • Gene Targeting Timeline
  • Generation of Chimeras Details
  • Repository Mice Available
• Pricing
• Methods
  • Beginners' Guide to Gene Targeting
  • Targeting Construct Linearization
  • Making Hot Probes For Southerns
  • Southern Blots with Alkaline Transfer
  • Southern Blots with Neutral Transfer
  • Generating a Transgenic Mouse
  • Preparing ES cells for Blastocyst Injection
  • Beginner's Guide to Mouse Management
  • Isolating and Culturing MEFs or Feeders
  • Blastocyst Harvest and Culture
  • Karyotyping a Cell Line
• Forms
• Mouse Models 101
  • Knockins and Knockouts
• Useful Links
• Contact Facility

Facility Sponsors

 

Rippel Mouse ES Cells and Transgenics Facility

This shared resource of the Koch Institute provides fee-for-service support to all MIT investigators who utilize novel mouse models to study human diseases such as cancer. Access is available to all members of the MIT community, to the extent permitted by available capacity. Priority access is given to KI members, NCI-funded research projects and other contributing user groups in recognition of funding support.

Services Include:

• Embryonic Stem Cell – gene targeting, assistance with vector design, importation and testing of lines from outside sources, generation of feeders and more.
• Microinjection – mouse ES cells into blastocysts, and DNA or virus into pronucleus of fertilized mouse eggs.
• Repository of Reagent Mice – containing Cre, Flp, EGFP, LacZ and other useful strains.
• Much more.

Services are scheduled on a first-come, first-served basis after approval.

Questions pertaining to our services?

Please email the Facility Director, Aurora Burds Connor.

The ES Cell & Transgenics Core is supported in part by funding provided to the Koch Institute from a National Cancer Institute Cancer Center Support Grant. We are thankful for the support that has been provided by the Fanny E. Rippel Foundation and by a grant from the National Institutes of Health to the Cell Migration Consortium.