Transformation of M15 cells (home-made cells) MSKC
Adapted from Quiagen- The Quiaexpressionist

1. Add 5 µL of DNA to the bottom of a pre-chilled Falcon 2059
2. Thaw M15[pREP4] cells on ice
3. Add 100 µL of cells to the DNA and allow to incubate on ice for 20 minutes
4. Heat shock the cells for 90 seconds at 42°C
5. Add 500 µL LB broth incubate for 1 hour shaking at 37°C
6. Plate 200 µL of cells on LB/Amp/Kan plates