CBB - last updated: September 1, 1998
(1) make lawn of 7155 using:
- two drops of plating bacteria
- added to 2.5 ml soft agar in small culture tube in
- vortex quickly
- spread on plate surface
- allow ~10 minutes to set
(2) sterilize platinum wire in flame.
(3) use a 1 ml glass pipet to touch surface of phage stock.
then touch center of a lawn transferring phage stock moisture to the
(4) use platinum wire to drag through the droplet in multiple
(5) place in 30degC room overnight.