CBB - last updated: September 2, 1998
|
STEP |
DAY |
|---|---|
|
Grow 7155 and 7136 on LB Plates Overnight |
1 |
|
Make 30-60 mls Overnight Culture of 7155 in LB Media |
2 |
|
Make Plating Bacteria |
3 |
|
Streak Phage From Master Stock |
3 |
|
Make Phage Stock |
4 |
|
Titer Phage Stock |
5 |
|
Make High Titer Phage Stock |
6 |
|
Titer High Titer Phage Stock |
7 |
|
Make Overnight Culture of 7136 in LB |
7 |
|
Infect Large Batch of 7136 with High Titer Phage Stock |
8 |
|
Harvest Cells and Freeze |
8 |
|
Freeze/Thaw |
9 |
|
Spins and AmSO4 Precipitation |
9 |
|
Spin and Dialysis |
10 |
|
DEAE Column (on FPLC - use recorder for analysis) |
11 |
|
AmSO4 Precipitation of DEAE product |
11 |
|
Spin and Dialysis |
12 |
|
HAP Column |
13 |
|
Analysis of HAP Fractions and Concentration |
14 |
FINAL PURIFIED PRODUCT