1x M9 Salts

Ingredient

Amount

%

Na2HPO4

232 g

0.6%

KH2PO4

120 g

0.3%

NaCl

20 g

0.05%

NH4Cl

40 g

0.01%

ddH2O

40 L

should be pH 7

Ingredient	Amount	%
Na2HPO4	232 g	0.6%
KH2PO4	120 g	0.3%
NaCl	20 g	0.05%
NH4Cl	40 g	0.01%
ddH2O	40 L

Minimal Media

Ingredient

[Stock]

to make 200 ml

to make 1 L

1x M9 Salts

above

MgSO4 (1mM)

1 M

0.2 ml

1 ml

FeCl3/CaCl2 (1µM)

1 mM *

0.2 ml

1 ml

Glucose (0.4%)

40%

2 ml

10 ml

Histidine (0.0015%)

0.3% *

1 ml

5 ml

Leucine (0.004%)

0.8% *

1 ml

5 ml

Yeast Extract (0.01%)

1%

2 ml

10 ml

* = make in lab (others from kitchen)

Ingredient	[Stock]	to make 200 ml	to make 1 L
1x M9 Salts	above
MgSO4 (1mM)	1 M	0.2 ml	1 ml
FeCl3/CaCl2 (1µM)	1 mM *	0.2 ml	1 ml
Glucose (0.4%)	40%	2 ml	10 ml
Histidine (0.0015%)	0.3% *	1 ml	5 ml
Leucine (0.004%)	0.8% *	1 ml	5 ml
Yeast Extract (0.01%)	1%	2 ml	10 ml