Education

Wheaton College, Norton, MA	B.A.	1981	Biology
Northeastern University, Boston MA	M.S.	1986	Pharmacology

Employment

Research Technician	Prof. Norman Boisse, Northeaster Univ..,	1981-1986
Technical Assistant	Prof. Jonathan King, Biology Dept. M.I.T....	1983-1987
Technical Associate	Prof. Jonathan King,, Biology Dept. M.I.T	1987- present

Professional Services

Chair of the M.I.T. Biology Depart. Safety Committee

1984-present

Publications

King, J. , Yu, M.-H., Siddiqi, J., and Haase, C. (1986). "Genetic identification of amino acid sequence influencing protein folding". In "Protein Engineering: Application in Science, Medicine and Industry" (M. Inouye, and R. .Sarma, Eds.). Academic Press, Orlando.

King, J. , and Haase-Pettingell, C. (1987). "Aggregate formation from thermolabile intermediates In the of the thermostable P22 tailspike". 623rd Meeting Of The Biochemical Society, Canterbury, July 16(2), 105-108.

Haase-Pettingell, C. A., and King, J. (1988). "Formation of aggregates from a thermolabile in-vivo folding intermediate in P22 tailspike maturation a model for inclusion formation". J Biol. Chem. 263(10), 4977-4983.

Sturtevant, J, Yu, M-h, Haase-Pettingell, C. and King, J. (1989) "Thermostability of temperature sensitive folding mutants of the P22 tailspike protein". J. Biol. Chem. 264, 10693 - 10698.

Friguet, B., Djavadi-Ohaniance, L., Haase-Pettingell, C. A., King, J. , and Goldberg, M. E. (1990). "Properties of monoclonal antibodies selected for probing the conformation of wild type and mutant forms of the P22 tailspike endorhamnosidase". Journal of Biological Chemistry 265(18), 10347-10351.

Mitraki, A., Fane, B., Haase-Pettingell, C., and King, J. (1991a). "Mutations affecting protein folding and misfolding in vivo". In "Application in Enzyme Biotechnology" (T. Baldwin, and J. Kelly, Eds.). Plenum Press, New York.

Mitraki, A., Haase-Pettingell, C., and King, J. (1991). "Mechanisms of inclusion body formation". In "Protein Refolding" (G. Georgiou, and DeBernardez-Clark, Eds.). ACS, Washington.

Mitraki, A., Fane, B., Haase-Pettingell, C., Sturtevant, J., and King, J. (1991b). "Global suppression of protein folding defects and inclusion formation". Science 253(5015), 54-58.

King, J. , Haase-Pettingell, C., Gordon, C., Sather, S., and Mitraki, A. (1993). "Amino acid sequence determination of polypeptide chain folding and inclusion body formation". In "Protein Folding, In vivo and in vitro." (J. Cleland, Ed.), Vol. 526. ACS, Washington, D. C.

Villafane, R., Fleming, A., and Haase-Pettingell, C. (1994). "Isolation of suppressors of temperature-sensitive folding mutations". J. Bact. 176, 137-142.

King, J., Teschke, C.M., Haase-Pettingell, C. and Mitraki, A. (1993) "Protein misfolding and inclusion body formation in prokaryotes." In: Research Opportunities in Biomolecular Engineering: The Interface Between chemical Engineering and Biology. Proceedings of the National Institute of General Medical Sciences. ( G. Georgiou and I. Glowinski, eds.), Washington, D.C., pp.25-32.

 

King, J., Haase-Pettingell, C., Robinson, A., Speed, M. A. and Mitraki, A. (1995) Thermolabile Folding Intermediates: Inclusion Body Precursors and Chaperonin Substrates. FASEB Journal, 10, 57-66.

 

Haase-Pettingell, C. and King, J. (1997) Prevalence of Temperature Sensitive folding Mutations in the parallel Beta Coil Domain of the Phage P22 Tailspike Endorhamnosidase. J. Mol. Biol., 267, 88-102.

 

Betts, S., Haase-Pettingell, C., and King, J. (1997) "Mutational effects on inclusion body formation." In, Protein Misassembly (ed. R. Wetzel), Volume 50 of "Advances in Protein Chemistry," Academic Press, pp 243-264.

Haase-Pettingell, C., Betts, S., Raso, S.W., Stuart, L., Robinson, A. and King, J. (2001) Role for cysteine residues in the in vivo folding and assembly of the phage P22 tailspike. Protein Science, 10, 397-410.

 

Raso, S.W., Clark, P.L., Haase-Pettingell, C., King, J. and Thomas, G.J., Jr. (2001) cysteine sulfhydryl environments of phage P22 tailspike rimer: analysis of raman S-H markers of Cys —› Ser mutant proteins. J. Mol. Biol., in press.

The interdigitated beta-helix domain of the P22 tailspike protein acts as a molecular clamp in trimer stabilization. Kreisberg, J.F., Betts, S. D., Haase-Pettingell, C. and King, J. (2002) Protein Science, 11, 820-830.

Understanding viral partitioning in two-phase aqueous nonionic micellar systems: 1. Role of attractive interactions between viruses and micelles. Kamei, D., Liu, C., Haase-Pettingell, C., King, J., Wang, D.I.C. & Blankschtein, D. (2002) Biotech & Bioeng, 78, 190-202.

Understanding viral partitioning in two-phase aqueous nonionic micellar systems: 2.Effect of entrained micelle-poor domains. Kamei, D., Liu, C., Haase-Pettingell, C., King, J., Wang, D.I.C. & Blankschtein, D. (2002) Biotech & Bioeng, 78, 203-216.

Protein Folding and Misfolding. King, J., Haase-Pettingell, C. & Gossard, D. (2002) American Scientist, 90, 445-453.

Pope, W., Haase-Pettingell, C. & King, J. (2004) Protein folding failure sets the high temperature limit on the growth of phage P22 in Salmonella serovar Typhimurium. Applied & Environmental Microbiology, in press.

Betts, S., Haase-Pettingell, C., Cook, K. and King, J. (2004) Buried hydrophobic side chains essential for the folding of the parallel beta-helix domains of the P22 tailspike. Protein Science, in press.

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