Y. Li, G.J. Thomas, Jr., M. Fuller and J. King (1981) Prog Clin Biol Res., 64, 271-283.

Investigations of bacteriophage P22 by laser Raman spectroscopy.


The laser-Raman spectrum of bacteriophage P22 consists of numerous lines due to radiation scattered by the normal modes of vibration of protein and DNA molecules of the virion. Each line is identified by its frequency shift (in cm-1 units) from the laser excitation frequency, and its intesity relative to an arbitrary standard. Among the many factors which determine the frequency and intensity of a given Raman line are the intramolecular and intermolecular interactions of the atoms of groups of atoms whose vibrations generate the Raman scattering. The Raman spectrum is therefore a useful means of investigating such interactions. The Raman method also benefits from the fact that sample homogeneity is not an absolute requirement for obtaining spectral data which can be interpreted directly. Therefore, clearcut advantages exist over S-ray diffraction and NMR or CD methods. In the case of P22 we have obtained Raman spectra from aqueous suspensions, slurries and gels, as well as solutions of phage and precursor particles.

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