Y. Li, G.J.
Thomas, Jr., M. Fuller and J. King (1981) Prog Clin Biol Res.,
Investigations of bacteriophage P22 by laser Raman spectroscopy.
laser-Raman spectrum of bacteriophage P22 consists of numerous lines due to
radiation scattered by the normal modes of vibration of protein and DNA molecules
of the virion. Each line is identified by its frequency shift (in cm-1 units)
from the laser excitation frequency, and its intesity relative to an arbitrary
standard. Among the many factors which determine the frequency and intensity
of a given Raman line are the intramolecular and intermolecular interactions
of the atoms of groups of atoms whose vibrations generate the Raman scattering.
The Raman spectrum is therefore a useful means of investigating such interactions.
The Raman method also benefits from the fact that sample homogeneity is not
an absolute requirement for obtaining spectral data which can be interpreted
directly. Therefore, clearcut advantages exist over S-ray diffraction and
NMR or CD methods. In the case of P22 we have obtained Raman spectra from
aqueous suspensions, slurries and gels, as well as solutions of phage and