Aggregation Intermediates

In vitro aggregation kinetics of P22 tailspike protein. Denatured tailspike chains in 5M urea, pH 3 were diluted 20-fold to 100ug/mL protein at 20C and 0.8M urea in 40mM sodium phosphate buffer, pH 7.6. Samples were placed on ice and then electrophoresed through a 9% acrylamide native gel run for 4hr at 4C. The amount of sample loaded was 1.3ug protein for the aggregation timepoints and 0.2ug for the native tailspike control. The protein band were visualized by the silver staining.

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