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> Cell-specific circuits

> Mechanisms of Rett Syndrome

> Neurons and astrocytes

> Structural correlates of cortical plasticity


dendritic spine on a layer V pyramidal neuron GFP labeled neuron orientation map of rewired A1

Cortical Development, Plasticity and Dynamics

Structural correlates of rapid cortical plasticity

Fundamental to understanding how neuronal circuits are created in cortex is ascertaining the mechanisms by which electrical activity is transduced into structural changes in neurons and connections. Primary visual cortex (V1) has been a proving ground for describing the phenomena and mechanisms of activity-dependent plasticity during development. Within V1, ocular dominance plasticity, particularly during an early, well-defined critical period, is a model for understanding functional and structural changes initiated by visual activity.

In one series of experiments, we are working to define the structural correlates of rapid functional plasticity during the critical period. In so doing, we seek to understand the mechanisms that sequentially transduce functional drive into structural changes in dendrites and axon terminals. In particular, spines are sites of the vast majority of excitatory synapses in cortex, but how their structure relates to functional plasticity in the intact cortex remains virtually unknown. We use the techniques of intrinsic signal optical imaging, high resolution two-photon laser scanning microscopy in vivo and in vitro, and viral expression of exogenous proteins, in ferrets and mice, to examine: (1) the time course of functional changes in the ferret visual cortex during the critical period for ocular dominance plasticity; (2) the structural correlates of rapid functional changes in the ferret visual cortex; (3) structural changes in spines with varying synaptic drive in ferret visual cortex; (4) functional and structural changes in the mouse visual cortex following short- and long-term term visual deprivation, including changes in different layers and specific cell classes; (5) specific molecular mechanisms, including the roles of CaMKII, actin and the extracellular matrix, involved in translating functional changes to structural reorganization in the visual cortex.

In these experiments, we are examining in unprecedented detail the extent and time course of structural changes at single synapses in the visual cortex to reveal mechanisms underlying their dynamic regulation by vision. Such information is critical for explaining pathologies of cortical development and for suggesting strategies for treatment.