Sauer Lab

The 1990s

1990

Sarah Ades

Papers written while in Sauer Lab:

• Tucker-Kellogg, L., Rould, M.A., Chambers, K.A., Ades, S.E., Sauer, R.T. & Pabo, C.O. (1997) Engrailed (Gln50ÆLys) homeodomain-DNA complex at 1.9 Å resolution: structural basis for enhanced affinity and altered specificity. Structure 5,1047-1054.
• Ades, S.E. & Sauer, R.T. (1995) Specificity of Minor-Groove and Major-Groove Interactions in a Homeodomain-DNA Complex Biochemistry 34, 14601- 14608.
• Ades, S.E. & Sauer, R.T. (1994) Differential DNA Binding Specificity of the Engrailed Homeodomain: the Role of Residue 50. Biochemistry 33, 9187- 9194.

Where she is now:

Pennsylvania State University

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Kenneth Keiler

Papers written while in Sauer Lab:

• Keiler, K. & Sauer, R.T. (2002) Tsp and Related C-terminal Processing Proteases. in The Enzymes, Vol. 22 Co- and Posttranslational Proteolysis of Proteins (Ross E. Dalbey, David S. Sigman eds.) Third Edition, Academic Press, 373-386.
• Keiler, K C. & Sauer, R.T. (1998) "Tail-specific protease" in Handbook of Proteolytic Enzymes (Woessner, F, Barrett, A.J., Rawlings, N.D., eds.). Academic Press, Orlando, FL.
• Keiler, K.C., Waller, P.R.H. & Sauer, R.T. (1996) Role of a peptide- tagging system in degradation of proteins translated from damaged mRNA. Science 271, 990-993.
• Keiler, K C. & Sauer, R.T. (1996) Sequence Determinants of C-terminal Substrate Recognition by the Tsp Protease. J. Biol. Chem. 271, 2589-2593.
• Keiler, K C. & Sauer, R.T. (1995) Identification of Active-Site Residues of the Tsp Protease J. Biol. Chem. 270, 28864-28868.
• Keiler, K.C., Silber, K.R., Downard, K.M., Papayannopoulos, I.A., Biemann, K., & Sauer, R.T. (1995) C-terminal Specific Protein Degradation: Activity and Substrate Specificity of the Tsp Protease. Protein Science 4, 1507-1515.
• Silber, K.R., Keiler, K.C. and Sauer, R.T. (1992). Tsp; a tail-specific protease that selectively degrades proteins with non-polar C-termini. Proc. Natl. Acad. Sci. USA 89, 295-299.

Where he is now:

Pennsylvania State University - bmb.psu.edu/faculty/keiler/keilerlab/keiler%20lab.htm

Email: kkeiler {at} psu.edu

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Marcos Milla

Papers written while in Sauer Lab:

• Sauer, R.T., Milla, M.E., Waldburger, C.D., Brown, B.M. & Schildbach, J.F. (1996) Sequence Determinants of Folding and Stability for the P22 Arc Repressor Dimer. FASEB Journal 10, 42-48.
• Milla, M.E., Brown, B.M., Waldburger, C.D. & Sauer, R.T. (1995) P22 Arc Repressor: Transition State Properties Inferred from Mutational Effects on the Rates of Protein Unfolding and Refolding. Biochemistry 34, 13914-13919.
• Schildbach, J.F., Milla, M.E., Jeffrey, P.D., Raumann, B.E., & Sauer, R.T. (1995) Crystal Structure, Folding, and Operator Binding of the Hyperstable Arc Repressor Mutant PL8. Biochemistry 34, 1405-1412.
• Milla, M.E. & Sauer, R.T. (1995) Critical Side-Chain Interactions at a Subunit Interface in the Arc Repressor Dimer Biochemistry 34, 3344-3351.
• Milla, M.E., Brown, B.M., & Sauer, R.T. (1994) Protein stability effects of a complete set of alanine substitutions in Arc repressor. Nature Struct. Biol. 1, 518-523.
• Brown, B.M., Milla, M.E., Smith, T.L. & Sauer, R.T. (1994) Scanning Mutagenesis of Arc Repressor as a Functional Probe of Operator Recognition. Nature Struct. Biol. 1, 164-168.
• Milla, M.E. and Sauer, R.T. (1994) P22 Arc Repressor: Folding Kinetics of a Single Domain, Dimeric Protein. Biochemistry 33, 1125-1133.
• Milla, M.E., Brown, B.M, and Sauer, R.T. (1993) P22 Arc Repressor: Enhanced Expression of Unstable Mutants by Addition of Polar C-terminal Sequences. Protein Science 2, 2198-2205.

Where he is now:

Roche Palo Alto, Department of Biochemical Pharmacology

Email: marcos.milla {at} roche.com

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Susan Marqusee

Paper written while in Sauer Lab:

• Marqusee, S. and Sauer, R.T. (1994) Dissection of Protein Stability: the Relative Contributions of Secondary and Tertiary Interactions Involving a Salt Bridge-Hydrogen Bond Network in the N-terminal Domain of l Repressor. Protein Science 3, 2217-2225.

Where she is now:

University of California, Berkeley

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1991

Lydia Gregoret

Papers written while in Sauer Lab:

• Gregoret, L.M. & Sauer, R.T. (1998) Tolerance of a protein helix to multiple alanine and valine substitutions. Folding & Design 3, 119-126.
• Gregoret, L. and Sauer, R.T. (1993) Additivity of Mutant Effects Assessed by Binomial Mutagenesis. Proc. Nat. Acad. Sci. USA 90, 4246-4250.

Where she is now:
Lydia left her tenured position at UC Santa Cruz and is now an at-home mom who does a little editing and writing on the side. She is very happy being at home with her kids and doesn't want to go back to full-time work for quite some time.

E-mail:
lydia.gregoret {at} alum.bu.edu

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Knut Langsetmo

Where he is now:

Boston Biomedical Research Institute - bbri.org/

Email: knut {at} bbri.org

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1992

Alan Davidson

Papers written while in Sauer Lab:

• Cordes, M.H.J., Davidson, A.R. & Sauer, R.T. (1996) Sequence Space, Folding, and Protein Design. Curr. Opinion Struct. Biol. 6, 3-10.
• Davidson, A.R., Lumb, K.J. & Sauer, R.T. (1995) Cooperatively Folded Proteins in Random Sequence Libraries. Nature Structural Biology 2, 856-863.
• Davidson, A.R. and Sauer, R.T. (1994) Folded Proteins Occur Frequently in Libraries of Random Amino Acid Sequences. Proc. Natl. Acad. Sci. USA 91, 2146-2150.

Where he is now:
University of Toronto - biochemistry.utoronto.ca/davidson/bch.html
Email: alan.davidson {at} sympatico.ca

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Tracy Smith Schmidt

Papers written while in Sauer Lab:

• Smith, T.L. & Sauer, R.T. (1996) Role of Operator Subsites in Arc Repression. J. Mol. Biol. 264, 233-242.
• Smith, T.L. & Sauer, R.T. (1996) Dual Regulation of Open-Complex Formation and Promoter Clearance by Arc Explains a Novel Repressor to Activator Switch. Proc. Nat. Acad. Sci. USA 93, 8868-8872.
• Smith, T.L. & Sauer, R.T. (1995) P22 Arc Repressor: Role of Cooperativity in Repression and Binding to Operators with Altered Half-Site Spacing. J. Mol. Biol. 249, 729-742.
• Brown, B.M., Milla, M.E., Smith, T.L. & Sauer, R.T. (1994) Scanning Mutagenesis of Arc Repressor as a Functional Probe of Operator Recognition. Nature Struct. Biol. 1, 164-168.

Where she is now:

Science writer

Email: tracysmithschmidt {at} yahoo.com

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Patrick Waller

Papers written while in Sauer Lab:

• Kolmar, H., Waller, P.R.H., & Sauer, R.T. (1996) The DegP and DegQ periplasmic endoproteases of Escherichia coli: Specificity for cleavage sites and substrate conformation. J. Bact. 178, 5925-5929.
• Keiler, K.C., Waller, P.R.H. & Sauer, R.T. (1996) Role of a peptide- tagging system in degradation of proteins translated from damaged mRNA. Science 271, 990-993.
• Waller, P.R.H. & Sauer, R.T. (1996) Characterization of degQ and degS, Escherichia coli genes encoding homologs of the DegP protease. J. Bact. 178, 1146-1153.

Where he is now:

Wolf, Greenfield & Sacks, P.C. - wolfgreenfield.com

Email: Patrick.Waller {at} WolfGreenfield.com

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Joel Schildbach

Papers written while in Sauer Lab:

• Schildbach, J.F., Karzai, A.W., Raumann, B.E. & Sauer, R.T. (1999) Origins of DNA Binding Specificity: Role of Protein Contacts with the DNA Backbone. Proc. Nat. Acad. Sci. USA 96, 811-817.
• Schildbach, J.F., Robinson, C.R. & Sauer, R.T. (1998) Biophysical characterization of the TraY protein of Escherichia coli F factor. J. Biol. Chem. 273, 1329-1333.
• Sauer, R.T., Milla, M.E., Waldburger, C.D., Brown, B.M. & Schildbach, J.F. (1996) Sequence Determinants of Folding and Stability for the P22 Arc Repressor Dimer. FASEB Journal 10, 42-48.
• Schildbach, J.F., Milla, M.E., Jeffrey, P.D., Raumann, B.E., & Sauer, R.T. (1995) Crystal Structure, Folding, and Operator Binding of the Hyperstable Arc Repressor Mutant PL8. Biochemistry 34, 1405-1412.
• Waldburger, C.D., Schildbach, J.F., & Sauer, R.T. (1995) Are Buried Salt Bridges Important for Protein Stability and Conformational Specificity. Nature Structural Biology 2, 122-128.

Where he is now:

Johns Hopkins University, Department of Biology

Email: joel {at} jhu.edu

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Carey Waldburger

Papers written while in Sauer Lab:

• Waldburger, C.D. & Sauer, R.T. (1996) Signal Detection by the PhoQ Sensor-Transmitter: Characterization of the Sensor Domain and a Response- Impaired Mutant that Identifies Ligand-Binding Determinants. J. Biol. Chem. 271, 26630- 26636.
• Jonsson, T., Waldburger, C.D. & Sauer, R.T. (1996) Nonlinear Free Energy Relationships in Arc Repressor Unfolding Imply Existence of Unstable, Native-like Folding Intermediates. Biochemistry 35, 4795-4802.
• Waldburger, C.D., Jonsson, T. & Sauer, R.T. (1996) Barriers to protein folding: Formation of buried polar interactions is a slow step in acquisition of structure. Proc. Natl. Acad. Sci U.S.A. 93, 2629-2634.
• Sauer, R.T., Milla, M.E., Waldburger, C.D., Brown, B.M. & Schildbach, J.F. (1996) Sequence Determinants of Folding and Stability for the P22 Arc Repressor Dimer. FASEB Journal 10, 42-48.
• Milla, M.E., Brown, B.M., Waldburger, C.D. & Sauer, R.T. (1995) P22 Arc Repressor: Transition State Properties Inferred from Mutational Effects on the Rates of Protein Unfolding and Refolding. Biochemistry 34, 13914-13919.
• Waldburger, C.D. & Sauer, R.T. (1995) Domains of Mnt Repressor: Roles in Tetramer Formation, Protein Stability, and Operator DNA Binding. Biochemistry 34, 13109-13116.
• Waldburger, C.D., Schildbach, J.F., & Sauer, R.T. (1995) Are Buried Salt Bridges Important for Protein Stability and Conformational Specificity. Nature Structural Biology 2, 122-128.

Where he is now:

Columbia University

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1994

Arie Berggrun

Papers written while in Sauer Lab:

• Berggrun, A. & Sauer, R.T. (2001) Contributions of Distinct Quaternary Contacts to Cooperative Operator Binding by Mnt Repressor. Proc. Natl. Acad. Sci. USA 98, 2301-2305.
• Berggrun, A. & Sauer, R.T. (2000) Interaction of Arg2 in Mnt’s N-terminal Arm with the Central and Flanking Regions of the mnt Operator. J. Mol. Biol. 301, 959-973.

Where he is now:

Kynikos Associates

Arie's e-mail

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Dionisios "Dennis" Rentzeperis

Papers written while in Sauer Lab:

• Rentzeperis, D., Jonsson, T., & Sauer, R.T. (1999) Acceleration of the Refolding of Arc Repressor by Nucleic Acids and Other Polyanions. Nature Structural Biology 6, 569 - 573.
• Robinson, C.R., Rentzeperis, D., Silva, J.R., & Sauer, R.T. (1997) Formation of a Denatured Dimer Limits the Thermal Stability of Arc Repressor. J. Mol. Biol. 273, 692-700.

Where he is now:

Johnson & Johnson PRD, L.L.C.

Email: drentze1 {at} prdus.jnj.com

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Matthew Cordes

Papers written while in Sauer Lab:

• Cordes, M.H.J., Walsh, N.P., McKnight,C.J.&. Sauer R.T. (2003) Solution structure of switch Arc, a mutant with 310 helices replacing a wild-type onβ-ribbon.
• Journal of Molecular Biology 326, 899-909.
• Cordes, M.H.J., Burton, R.E., Walsh, N.P., McKnight, C.J. & Sauer, R.T. (2000) An Evolutionary Bridge to a New Protein Fold. Nature Structural Biology 7, 1129-1132.
• Cordes, M.J., Walsh, N., McKnight, J. & Sauer, R.T. (1999) Evolution of a Protein Fold In Vitro. Science 284, 325-327.
• Cordes, M.J. & Sauer, R.T. (1999) Tolerance of a Protein to Multiple Hydrophobic to Polar Surface Substitutions. Protein Science 8, 318-325.
• Cordes, M.H.J., Davidson, A.R. & Sauer, R.T. (1996) Sequence Space, Folding, and Protein Design. Curr. Opinion Struct. Biol. 6, 3-10.

Where he is now:

University of Arizona - biochem.arizona.edu/dept/ppl/Profiles/cordes.htm

Email: cordes {at} u.arizona.edu

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Thorlakur Jonsson

Papers written while in Sauer Lab:

• Rentzeperis, D., Jonsson, T., & Sauer, R.T. (1999) Acceleration of the Refolding of Arc Repressor by Nucleic Acids and Other Polyanions. Nature Structural Biology 6, 569 - 573.
• Hendsch, Z.S., Jonsson, T., Sauer, R.T. & Tidor, B. (1996) Protein Stabilization By Removal of Unsatisfied Polar Groups: Computational Approaches and Experimental Tests. Biochemistry 35, 7621-7625.
• Jonsson, T., Waldburger, C.D. & Sauer, R.T. (1996) Nonlinear Free Energy Relationships in Arc Repressor Unfolding Imply Existence of Unstable, Native-like Folding Intermediates. Biochemistry 35, 4795-4802.
• Waldburger, C.D., Jonsson, T. & Sauer, R.T. (1996) Barriers to protein folding: Formation of buried polar interactions is a slow step in acquisition of structure. Proc. Natl. Acad. Sci U.S.A. 93, 2629-2634.

Where he is now:

DeCode Genetics - decode.is/

Email: thorlakur.jonsson {at} decode.is

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Harald Kolmar

Papers written while in Sauer Lab:

• Kolmar, H., Waller, P.R.H., & Sauer, R.T. (1996) The DegP and DegQ periplasmic endoproteases of Escherichia coli: Specificity for cleavage sites and substrate conformation. J. Bact. 178, 5925-5929.

Where he is now:

University of Göttingen

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Clifford Robinson

Postdoc

Papers written while in Sauer Lab:

• Robinson, C.R. & Sauer, R.T. (2000) Striking Stabilization of Arc Repressor by an Engineered Disulfide Bond. Biochemistry 39, 12494-502.
• Robinson, C.R. & Sauer, R.T. (1998) Optimizing the Stability of Single-Chain Proteins by Linker Length and Composition Mutagenesis. Proc. Natl. Acad. Sci. USA 95, 5929-5934.
• Schildbach, J.F., Robinson, C.R. & Sauer, R.T. (1998) Biophysical characterization of the TraY protein of Escherichia coli F factor. J. Biol. Chem. 273, 1329-1333.
• Robinson, C.R., Rentzeperis, D., Silva, J.R., & Sauer, R.T. (1997) Formation of a Denatured Dimer Limits the Thermal Stability of Arc Repressor. J. Mol. Biol. 273, 692-700.
• Robinson, C.R. & Sauer, R.T. (1996) Equilibrium Stability and Sub-Millisecond Refolding of a Designed Single-Chain Arc Repressor. Biochemistry 35, 13878-13884.
• Robinson, C.R. & Sauer, R.T. (1996) Covalent Attachment of Arc Repressor Subunits by a Peptide Linker Enhances Affinity for Operator DNA. Biochemistry 35, 109-116.

Where he is now:

University of Delaware - dbi.udel.edu/robinson_c.html

Email: robinson {at} dbi.udel.edu

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1995

Alix Van Geel

Where she is now:

Industrial Economics

Email: avangeel {at} indecon.com

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Eric Roche

Papers written while in Sauer Lab:

• Roche, E.D. & Sauer, R.T. (2001) Identification of endogenous SsrA-tagged proteins reveals tagging at positions corresponding to stop codons. J. Biol Chem. 276, 28509-28515.
• Karzai, A.W., Roche, E.D. & Sauer, R.T. (2000) The SsrA-SmpB System for Protein Tagging, Directed Degradation, and Ribosome Rescue. Nature Structural Biology 7, 449-455.
• Roche, E.D. & Sauer, R.T. (1999) SsrA-mediated Tagging Caused by Rare Codons and tRNA Scarcity. EMBO J. 18, 4579-4589.
• Gottesman, S., Roche, E., Zhou, Y.N., & Sauer, R.T. (1998) The ClpXP and ClpAP proteases degrade proteins with C-terminal peptide tails added by the SsrA tagging system. Genes & Development 12, 1338-1347.

Where he is now:

Cumbre, Inc. - cumbre.net

Email: eric.roche {at} cumbre.biz

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Alok Srivastava

Postdoc

Papers written while in Sauer Lab:

• Srivastava, A.K., & Sauer, R.T. (2002) Mutational Studies of Protein Stability and Folding of the Hyperstable MYL Arc Repressor Variant. Biophys.Chem. (in press)
• Srivastava, A.K. & Sauer, R.T. (2000) Evidence for Partial Secondary Structure Formation in the Transition State for Arc Repressor Refolding and Dimerization. Biochemistry 39, 8308-8314.

Where he is now:

BioScale, Inc. - bioscale.com/

Email: shree_alok {at} yahoo.com

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Wali Karzai

Papers written while in Sauer Lab:

• Barends, S., Karzai, A.W., Sauer, R.T., Wower, J. & Kraal, B. (2001) Simultaneous and Functional Binding of SmpB and EF-TU to the Alanyl Acceptor Arm of tmRNA. J. Mol. Biol. 314, 9-21.
• Karzai, A.W. & Sauer, R.T. (2001) Protein Factors Associated with the SsrA•SmpB Tagging and Ribosome Rescue Complex. Proc. Natl. Acad. Sci. USA 98, 3040-3044.
• Karzai, A.W., Roche, E.D. & Sauer, R.T. (2000) The SsrA-SmpB System for Protein Tagging, Directed Degradation, and Ribosome Rescue. Nature Structural Biology 7, 449-455.
• Karzai, A.W. & Sauer, R.T. (1999) SmpB, A Unique RNA-Binding Protein Essential for the Peptide-Tagging Activity of SsrA (tmRNA). EMBO J. 18, 3793-3799.
• Schildbach, J.F., Karzai, A.W., Raumann, B.E. & Sauer, R.T. (1999) Origins of DNA Binding Specificity: Role of Protein Contacts with the DNA Backbone. Proc. Nat. Acad. Sci. USA 96, 811-817.

Where he is now:

State University of New York- sunysb.edu/biochem/BIOCHEM/facultypages/Karzai.html

Email: akarzai {at} ms.cc.sunysb.edu

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1996

Virginia Cornish

Paper written while in Sauer Lab:

• Lin, H., Abida, W.A., Sauer, R.T. & Cornish, V.W. (2000) Dexamethasone-Methotrexate: An Efficient Chemical Inducer of Protein Dimerization In Vivo. J. Amer. Chem. Soc. 122, 4247-4248.

Where she is now:

Columbia University - columbia.edu/cu/chemistry/groups/cornish/index.html

Email: vc114 {at} columbia.edu

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Nathan Walsh

Papers written while in Sauer Lab:

• Cordes, M.H.J., Burton, R.E., Walsh, N.P., McKnight, C.J. & Sauer, R.T. (2000) An Evolutionary Bridge to a New Protein Fold. Nature Structural Biology 7, 1129-1132.
• Cordes, M.J., Walsh, N., McKnight, J. & Sauer, R.T. (1999) Evolution of a Protein Fold In Vitro. Science 284, 325-327.
• Levchenko, I., Smith, C.K., Walsh, N.P., Sauer, R.T. & Baker, T.A. (1997) PDZ-like Domains Mediate Binding Specificity in the Clp/Hsp100 Family of Chaperones and Protease Regulatory Subunits. Cell 91, 939-947.

Where he is now:

Brigham & Women's Hospital

Email: nwalsh {at} genetics.med.harvard.edu

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1997

Peter Chivers

Papers written while in Sauer Lab:

• Chivers, P.T. & Sauer, R.T. (2002) NikR repressor: high-affinity nickel binding to the C-terminal domain regulates binding to operator DNA. submitted.
• Chivers, P.T. & Sauer, R.T. (2000) Regulation of High-Affinity Nickel Uptake in Bacteria: Ni2+-Dependent Interaction of NikR with Wild-type and Mutant Operator Sites. J. Biol. Chem. 275, 19735-19741.
• Chivers, P.T. & Sauer, R.T. (1999) NikR is a ribbon-helix-helix DNA-binding protein. Protein Science 8, 2494-2500.

Where he is now:

Washington University

Email: chivers {at} wustl.edu

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Michael Nohaile

Papers written while in Sauer Lab:

• Nohaile, M.J., Hendsch, Z.S., Tidor, B. & Sauer, R.T. (2001) Altering Dimerization Specificity by Changes in Surface Electrostatics. Proc. Natl. Acad. Sci. USA 98, 3109-3114.
• Hendsch, Z.S., Nohaile, M.J., Sauer, R.T., & Tidor, B. (2001) Preferential Heterodimer Formation Via Undercompenstated Electrostatic Interactions. J. Amer. Chem. Soc. 123, 1264-1265.

Where he is now:

McKinsey & Company, Inc.

Email: Michael_Nohaile {at} mckinsey.com

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Catherine Smith

Papers written while in Sauer Lab:

• Smith, C.K., Wohnert, J., Sauer, R.T. & Schwalbe, H. (2001) Assignments of the 1H, 13C, and 15N resonances of the substrate-binding SSD domain from Lon protease. Journal of Biomolecular NMR 21, 387-388.
• Smith, C.K., Baker, T.A., & Sauer, R.T. (1999) Lon and Clp Family Proteases and Chaperones Share Homologous Substrate-Recognition Domains. Proc. Natl. Acad. Sci. USA 96, 6678-6682.
• Levchenko, I., Smith, C.K., Walsh, N.P., Sauer, R.T. & Baker, T.A. (1997) PDZ-like Domains Mediate Binding Specificity in the Clp/Hsp100 Family of Chaperones and Protease Regulatory Subunits. Cell 91, 939-947.

Where she is now:

Schering- Plough

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Ehud Gazit

Papers written while in Sauer Lab:

• Gazit, E. & Sauer, R.T. (1999) The Doc Toxin and Phd Antidote Proteins of the Bacteriophage P1 Plasmid Addiction System Form a Heterotrimeric Complex. J. Biol. Chem. 274,16813-16818.
• Gazit, E. & Sauer, R.T. (1999) Stability and DNA Binding of the Phd Protein of the Phage P1 Plasmid Addiction System. J. Biol. Chem. 274, 2652-2657.

Where he is now:

Tel-Aviv University - tau.ac.il/lifesci/departments/biotech/members/gazit/gazit.html

Email: EhudGa {at} tauex.tau.ac.il

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John Lo

Papers written while in Sauer Lab:

• Lo, J., Baker, T.A. & Sauer, R.T. (2001) Characterization of the N-terminal Repeat Domain of E. coli ClpA—a Class I Clp/HSP100 ATPase. Protein Science 10, 551-559.

Where he is now:

McKinsey & Company, Inc.

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1998

Ramon Tabtiang

Papers written:

• Tabtiang, R.K., Cezairliyan, B.O., Grant, R.A., Cochrane, J.C. & Sauer, R.T. (2005) Consolidating critical binding determinants by non-cyclic rearrangement of protein secondary structure. Proc. Natl. Acad. Sci. USA 102, 2305-2309.

Where he is now:

Fish & Richardson, P.C.

Email: tabtiang {at} fr.com

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Tony Anderson

Papers written while in Sauer Lab:

• Anderson, T.A. & Sauer, R.T. (2002) Role of an Ncap Residue in Determining the Stability and Operator-Binding Affinity of Arc Repressor. submitted.

Where he is now:

University of Michigan Medical School

Email: tanthonya {at} yahoo.com

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Shilpa Joshi

Graduate Student

Papers written while in Sauer Lab:

• Joshi, S.A., Hersch G.L., Baker, T.A., & Sauer, R.T. (2004) Communication between ClpX and ClpP during substrate processing and degradation. Nat. Struct. Mol. Biol., 11, 404-411.
• Joshi, S.A., Baker, T.A., & Sauer, R.T. (2003) C-terminal domain mutations in ClpX uncouple substrate binding from an engagement step required for unfolding. Mol. Microbiol., 48, 67-76.

Where she is now:

She's @ UCSF.

Email: sjoshi {at} itsa.ucsf.edu

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Randy Burton

Research Scientist

Papers written while in Sauer Lab:

• Burton, R.E., Baker, T.A., Sauer, R.T. (2005) Nucleotide-dependent substrate recognition by the AAA+ HsIUV protease. Nature Structural and Molecular Biology, 12: 245-251.
• Kenniston, J.A., Burton, R.E., Siddiqui, S.M., Baker, T.A. & Sauer, R.T. (2004) Effects of local protein stability and the geometric position of the substrate degradation tag on the efficiency of ClpXP denaturation and degradation. J. Struct. Biol. 146, 130-140.
• Burton, R.E., Baker, T.A. & Sauer, R.T. (2003) Energy-dependent degradation: Linkage between ClpX-catalyzed nucleotide hydrolysis and protein-substrate processing. Protein Sci. 12, 893-902.
• Burton, R.E., Siddiqui, S.M., Kim, Y.I., Baker, T.A. & Sauer, R.T. (2001) Effects of protein stability and structure on substrate processing by the ClpXP unfolding and degradation machine. EMBO Journal 20, 3092-3100.
• Cordes, M.H.J., Burton, R.E., Walsh, N.P., McKnight, C.J. & Sauer, R.T. (2000) An Evolutionary Bridge to a New Protein Fold. Nature Structural Biology 7, 1129-1132.
• Kim, Y.I., Burton, R.E., Burton, B.M., Sauer, R.T. & Baker, T.A. (2000) Dynamics of Substrate Denaturation and Translocation by the ClpXP Degradation Machine. Molecular Cell 5, 639-648.

Where he is now:

US Genomics

Email: reb1 {at} mit.edu

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1999

Shari Spector

Appointed to 2 labs: Sauer Lab in Biology and Tidor Lab in EECS/BEH.

Papers written while in Sauer Lab:

• Spector, S., Sauer, R.T., & Tidor, B. (2004) Computational and experimental probes of symmetry mismatches in the Arc repressor-DNA complex. J Mol. Biol. 340, 253-261.
• Spector, S., Flynn, J.M., Tidor, B., Baker, T.A., & Sauer, R.T. (2003) Expression of N-formylated proteins in Escherichia coli. Protein Expr. Purif. 32, 317-322.

Where she is now:

Millipore Corporation

Email: sharispector {at} rcn.com

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