Bob Sauer's Lab

BRONWEN BROWN LOEB

Bronwen (Brown) Loeb
Graduate Student (1988-1994)
Email (Bronwen.Loeb at dbr.com)

Patent Agent
Drinker Biddle & Reath, LLP

Brown, B.M. & Sauer, R.T. (1999) Tolerance of Arc Repressor to Multiple Alanine Substitutions. Proc. Natl. Acad. Sci. USA 96, 1983-1988. pubmed. citation

Sauer, R.T., Milla, M.E., Waldburger, C.D., Brown, B.M. & Schildbach, J.F. (1996) Sequence Determinants of Folding and Stability for the P22 Arc Repressor Dimer. FASEB Journal 10, 42-48. pubmed. citation

Milla, M.E., Brown, B.M., Waldburger, C.D. & Sauer, R.T. (1995) P22 Arc Repressor: Transition State Properties Inferred from Mutational Effects on the Rates of Protein Unfolding and Refolding. Biochemistry 34, 13914-13919. pubmed. citation

Milla, M.E., Brown, B.M., & Sauer, R.T. (1994) Protein stability effects of a complete set of alanine substitutions in Arc repressor. Nature Struct. Biol. 1, 518-523. pubmed. citation

Brown, B.M., Milla, M.E., Smith, T.L. & Sauer, R.T. (1994) Scanning Mutagenesis of Arc Repressor as a Functional Probe of Operator Recognition. Nature Struct. Biol. 1, 164-168. pubmed. citation

Raumann, B.E., Brown, B.M. & Sauer, R.T. (1994) Major Groove DNA Recognition by b-sheets: The Ribbon-Helix-Helix Family of Gene Regulatory Proteins. Curr. Opin. Struct. Biol. 4, 36-43. pubmed. citation

Milla, M.E., Brown, B.M., and Sauer, R.T. (1993) P22 Arc Repressor: Enhanced Expression of Unstable Mutants by Addition of Polar C-terminal Sequences. Protein Science 2, 2198-2205. pubmed. citation

Brown, B.M. and Sauer, R.T. (1993) Assembly of the Arc Repressor-Operator Complex: Cooperative Interactions between DNA-Bound Dimers. Biochemistry 32, 1354-1363. pubmed. citation

Brown, B.M., Bowie, J.U. & Sauer , R.T. (1990) Arc Repressor is Tetrameric When Bound to Operator DNA. Biochemistry 29, 11189-11195. pubmed. citation

KEVIN SHOEMAKER

Kevin Shoemaker
Postdoc (1988-1991)
Email (kevin.shoemaker at novartis.com)

Research Director
Norvartis Institutes for BioMedical Research

Reidhaar-Olson, J.F., Bowie, J.U., Breyer, R.M., Hu, J.C., Knight, K.L., Lim, W.A., Mossing, M.C., Parsell, D.A., Shoemaker, K.R.& Sauer, R.T. (1991). Random Mutagenesis of Protein Sequences Using Oligonucleotide Cassettes. in Methods in Enzymology vol 208 (Sauer, R.T., ed.) pps. 564-586, Academic Press, Orlando, FL.

KAREN (SILBER) WEISSEL

Karen (Silber) Weissel
Graduate Student (1988-1992)
Email (weissel05 at comcast.net)

Karen left an intellectual property management/technology transfer position at a biotech company and is now at home with three children.

Keiler, K.C., Silber, K.R., Downard, K.M., Papayannopoulos, I.A., Biemann, K., & Sauer, R.T. (1995) C-terminal Specific Protein Degradation: Activity and Substrate Specificity of the Tsp Protease. Protein Science 4, 1507-1515.

Silber, K.R. and Sauer, R.T. (1994) Deletion of the prc (tsp) gene provides evidence for additional tail-specific proteolytic activity in Escherichia coli. K12 Mol. Gen. Genet. 242, 237-240.

Silber, K.R., Keiler, K.C. and Sauer, R.T. (1992). Tsp; a tail-specific protease that selectively degrades proteins with non-polar C-termini. Proc. Natl. Acad. Sci. USA 89, 295-299.

Parsell, D.A., Silber, K.R. & Sauer, R.T. (1990). C-terminal Determinants of Intracellular Protein Degradation. Genes and Development 4, 277-286. pubmed. citation

WENDELL LIM

Wendell Lim
Graduate Student (1987-1991)
Email (lim at cmp.ucsf.edu)

Faculty
University of California, San Francisco

Lim, W.A., Hodel, A., Sauer, R.T. and Richards, F.M. (1994) The Crystal Structure of a Mutant Protein with Altered but Improved Hydrophobic Core Packing Proc. Natl. Acad. Sci. USA 91, 423-427. pubmed citation

Sauer, R.T. and Lim, W.A. (1992) Mutational Analysis of Protein Stability. Current Opinion in Structural Biology 2, 46-51. pubmed citation

Lim, W.A., Farruggio, D.C. and Sauer, R.T. (1992) The Structural and Energetic Consequences of Disruptive Mutations in a Protein Core. Biochemistry 31, 4324-4333. pubmed citation

Lim, W.A., Sauer, R.T., and Lander, A.D. (1991) Analysis of DNA-Protein Interactions by Affinity Co-Electrophoresis. in Methods in Enzymology vol 208 (Sauer, R.T., ed.) pps. 196-210, Academic Press, Orlando, FL. pubmed citation

Reidhaar-Olson, J.F., Bowie, J.U., Breyer, R.M., Hu, J.C., Knight, K.L., Lim, W.A., Mossing, M.C., Parsell, D.A., Shoemaker, K.R.& Sauer, R.T. (1991). Random Mutagenesis of Protein Sequences Using Oligonucleotide Cassettes. in Methods in Enzymology vol 208 (Sauer, R.T., ed.) pps. 564-586, Academic Press, Orlando, FL pubmed citation

Lim, W.A. & Sauer, R.T. (1991). The Role of Internal Packing Interactions in Determining the Structure and Stability of a Protein. J. Mol. Biol. 219, 359-376. pubmed citation

Bowie, J.U., Reidhaar-Olson, J.F., Lim, W.A. & Sauer, R.T. (1990). Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions. Science 247, 1306-1310. pubmed citation

Lim, W.A. & Sauer, R.T. (1989). Alternative Packing Arrangements in the Hydrophobic Core of l Repressor. Nature 339, 31-36. pubmed citation

JIM HU

Jim Hu
Postdoc (1987-1992)
Email (jimhu at tamu.edu)

Faculty
Texas A&M University

Hu, J.C., Newell, N.E., Tidor, B. and Sauer, R.T. (1993) Probing the Roles of Residues at the e and g Positions of the GCN4 Leucine Zipper by Combinatorial Mutagenesis. Protein Science 2, 1072-1084. pubmed. citation

Hu, J.C. and Sauer, R.T. (1992) The basic-region leucine-zipper family of DNA binding proteins. in Nucleic Acids and Molecular Biology vol VI (Eckstein, F. and Lilley, D.M.J., eds.) Springer-Verlag, Berlin, pps. 82-101.

Reidhaar-Olson, J.F., Bowie, J.U., Breyer, R.M., Hu, J.C., Knight, K.L., Lim, W.A., Mossing, M.C., Parsell, D.A., Shoemaker, K.R.& Sauer, R.T. (1991). Random Mutagenesis of Protein Sequences Using Oligonucleotide Cassettes. in Methods in Enzymology vol 208 (Sauer, R.T., ed.) pps. 564-586, Academic Press, Orlando, FL.

Hu, J.C., O’Shea, E.K., Kim, P.S., and Sauer, R.T. (1990) Sequence Requirements for Coiled-Coil Interactions: Analysis Using l Repressor-GCN4 Leucine Zipper Fusions. Science 250, 1400-1403.

DOROTHY BECKETT

Dorothy Beckett
Postdoc (1986-1987)
Email (dbeckett at umd.edu)

Faculty
University of Maryland

Beckett, D., Burz, D.S., Ackers, G.K., and Sauer, R.T. (1993) Isolation of lambda repressor mutants with defects in cooperative operator binding. Biochemistry 32, 9073-9079. pubmed. citation

MIKE MOSSING

Michael Mossing
Postdoc (1986-1990)
Email (mmossing at olemiss.edu)

Faculty
University of Mississippi

Mossing, M.C., Bowie, J.U. & Sauer, R.T. (1991) A Streptomycin Selection for DNA Binding Activity. in Methods in Enzymology vol 208 (Sauer, R.T., ed.) pps. 604-619, Academic Press, Orlando, FL.

Reidhaar-Olson, J.F., Bowie, J.U., Breyer, R.M., Hu, J.C., Knight, K.L., Lim, W.A., Mossing, M.C., Parsell, D.A., Shoemaker, K.R.& Sauer, R.T. (1991). Random Mutagenesis of Protein Sequences Using Oligonucleotide Cassettes. in Methods in Enzymology vol 208 (Sauer, R.T., ed.) pps. 564-586, Academic Press, Orlando, FL.

Mossing, M.C. and Sauer, R.T. (1990). Stable, Monomeric Variants of l Cro Obtained by Insertion of a Designed b-Hairpin Sequence. Science 250, 1712-1715.

JIM BOWIE

James Bowie
Graduate Student (1985-1989)
Email (bowie at mbi.ucla.edu)

Faculty
University of California Los Angeles

Mossing, M.C., Bowie, J.U. & Sauer, R.T. (1991) A Streptomycin Selection for DNA Binding Activity. Methods in Enzymology vol 208 (Sauer, R.T., ed.) pps. 604-619, Academic Press, Orlando, FL. pubmed citation

Reidhaar-Olson, J.F., Bowie, J.U., Breyer, R.M., Hu, J.C., Knight, K.L., Lim, W.A., Mossing, M.C., Parsell, D.A., Shoemaker, K.R.& Sauer, R.T. (1991). Random Mutagenesis of Protein Sequences Using Oligonucleotide Cassettes. Methods in Enzymology vol 208 (Sauer, R.T., ed.) pps. 564-586, Academic Press, Orlando, FL. 189

Brown, B.M., Bowie, J.U. & Sauer, R.T. (1990) Arc Repressor is Tetrameric When Bound to Operator DNA. Biochemistry 29, 11189-11195.

Bowie, J.U., Clarke, N.D, Pabo, C.O. & Sauer, R.T. (1990). Identification of Protein Folds: Matching Hydrophobicity Patterns of Sequence Sets with Solvent Accessibility Patterns of Known Structures. Proteins: Structure, Function, and Genetics 7, 257-264.

Bowie, J.U. & Sauer, R.T. (1990). TraY Proteins of F and Related Episomes are Members of the Arc and Mnt Repressor Family. J. Mol. Biol. 211, 5-6. pubmed citation

Bowie, J.U., Reidhaar-Olson, J.F., Lim, W.A. & Sauer, R.T. (1990). Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions. Science 247, 1306-1310. pubmed citation

Zagorski, M.G., Bowie, J.U., Vershon, A.K., Sauer, R.T. & Patel, D.J. (1989) NMR Studies of the Arc Repressor Mutant PL8: Resonance Assignments, Secondary Structure and Long Range Contacts. Biochemistry 28, 9813-9825. pubmed citation

Bowie, J.U. & Sauer, R.T. (1989) Equilibrium Dissociation and Unfolding of the Arc Repressor Dimer. Biochemistry 28, 7139-7143. pubmed citation

Bowie, J.U. & Sauer, R.T. (1989). Identification of C-terminal Extensions That Protect Proteins from Intracellular Proteolysis. J. Biol. Chem. 264, 7596-7602. pubmed citation

Knight, K.L., Bowie, J.U., Vershon, A.K., Kelley, R.D. & Sauer, R.T. (1989). The Arc and Mnt Repressors: A New Class of Sequence Specific DNA-Binding Protein. J. Biol. Chem. 264, 3639-3642. pubmed citation

Bowie, J.U. & Sauer, R.T. (1989). Identifying Determinants of Folding and Activity for a Protein of Unknown Structure. Proc. Natl. Acad. Sci. U.S.A. 86, 2152-2156. pubmed citation

Vershon, A.K., Bowie, J.U., Karplus, T.M. and Sauer, R.T. (1986). Isolation and Analysis of Arc Repressor Mutants: Evidence for an Unusual Mechanism of DNA Binding. Proteins: Structure, Function & Genetics 1, 302-311. pubmed citation

ROBIN (KELLEY) KELSON

Robin (Kelley) Kelson
Graduate Student (1986-1988)
Email (vrkelson at montanasky.com )

Founder/Director
U14/5 HERBS ~ plant studies for humankind

Knight, K.L., Bowie, J.U., Vershon, A.K., Kelley, R.D. &Sauer, R.T. 1989. The Arc and Mint Repressors: A New Class of Sequence Specific DNA-Binding Protein, J. Biol. Chem. 264, 3639-3642. pubmed. citation

Vershon, A.K., Kelley, R.D. , & Sauer, R.T.. (1989) Sequence-specific Binding of Arc Repressor to DNA: Effects of Operataor Mutations and Modifications. J. Biol. Chem. 264, 3267-3273. pubmed. citation

JOHN REIDHAAR-OLSON

John Reidhaar-Olson
Graduate Student (1985-1990)
Email (john.reidhaar-olson at ROCHE.COM)

Research Scientist
Hoffmann-La Roche

Reidhaar-Olson, J.F., Bowie, J.U., Breyer, R.M., Hu, J.C., Knight, K.L., Lim, W.A., Mossing, M.C., Parsell, D.A., Shoemaker, K.R.& Sauer, R.T. (1991). Random Mutagenesis of Protein Sequences Using Oligonucleotide Cassettes. in Methods in Enzymology vol 208 (Sauer, R.T., ed.) pps. 564-586, Academic Press, Orlando, FL.

Reidhaar-Olson, J.F., Parsell, D.A. & Sauer, R.T. (1990) An Essential Proline in l Repressor is Required for Resistance to Intracellular Proteolysis. Biochemistry 29, 7563-7571. pubmed. citation

Reidhaar-Olson, J.F. & Sauer, R.T. (1990). Functionally Acceptable Substitutions in Two a-helical Regions of l Repressor. Proteins: Structure, Function, and Genetics 7, 306-316. pubmed. citation

Bowie, J.U., Reidhaar-Olson, J.F., Lim, W.A. & Sauer, R.T. (1990). Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions. Science 247, 1306-1310. pubmed. citation

Reidhaar-Olson, J.F. and Sauer, R.T. (1988). Combinatorial Cassette Mutagenesis as a Probe of the Informational Content of Protein Sequences. Science 241, 53-57. pubmed. citation

DAWN PARSELL

Dawn Parsell
Graduate Student (1985-1990)
Email (dp1571 at austin.rr.com)

Regulatory Affairs Consultant

Reidhaar-Olson, J.F., Bowie, J.U., Breyer, R.M., Hu, J.C., Knight, K.L., Lim, W.A., Mossing, M.C., Parsell, D.A., Shoemaker, K.R.& Sauer, R.T. (1991). Random Mutagenesis of Protein Sequences Using Oligonucleotide Cassettes. Methods in Enzymology 208 (Sauer, R.T., ed.) pps. 564-586, Academic Press, Orlando, FL.

Reidhaar-Olson, J.F., Parsell, D.A. & Sauer, R.T. (1990) An Essential Proline in l Repressor is Required for Resistance to Intracellular Proteolysis. Biochemistry 29, 7563-7571. pubmed. citation

Parsell, D.A., Silber, K.R. & Sauer, R.T. (1990). C-terminal Determinants of Intracellular Protein Degradation. Genes and Development 4, 277-286. pubmed. citation

Parsell, D.A. & Sauer, R.T. (1989). Induction of a Heat Shock-like Response by Unfolded Protein in E. coli: Dependence on Protein Level Not Protein Degradation. Genes and Development 3, 1226-1232. pubmed. citation

Parsell, D.A. & Sauer, R.T. (1989). The Structural Stability of a Protein Is an Important Determinant of Its Proteolytic Susceptibility in E. coli. J. Biol. Chem. 264, 7590-7595. pubmed. citation

KEN KNIGHT

NO PHOTO AVAILABLE

Kendall Knight
Postdoc (1985-1989)
Email (Kendall.Knight at umassmed.edu)

Faculty
University of Massachusetts Medical School

Raumann, B.E., Knight, K.L. & Sauer, R.T. (1995) Dramatic Changes in DNA-Binding Specificity Caused By Single Residue Substitutions in an Arc/Mnt Hybrid Repressor. Nature Structural Biology 2, 1115-1122. pubmed. citation

Burgering, M.J., Boelens, R., Gilberyt, D.E., Breg, J.N., Knight, K.L., Sauer, R.T. & Kaptein, R. (1994) Solution Structure of Dimeric Mnt Repressor (1-76). Biochemistry 33, 15036-15045. pubmed. citation

Knight, K.L. and Sauer, R.T. (1992) Biochemical and Genetic Analysis of Operator Contacts Made by Residues within the b-sheet DNA Binding Motif of Mnt Repressor EMBO J. 11, 215-223. pubmed. citation

Reidhaar-Olson, J.F., Bowie, J.U., Breyer, R.M., Hu, J.C., Knight, K.L., Lim, W.A., Mossing, M.C., Parsell, D.A., Shoemaker, K.R.& Sauer, R.T. (1991). Random Mutagenesis of Protein Sequences Using Oligonucleotide Cassettes. in Methods in Enzymology vol 208 (Sauer, R.T., ed.) pps. 564-586, Academic Press, Orlando, FL. pubmed. citation

Knight, K.L., Bowie, J.U., Vershon, A.K., Kelley, R.D. & Sauer, R.T. (1989). The Arc and Mnt Repressors: A New Class of Sequence Specific DNA-Binding Protein. J. Biol. Chem. 264, 3639-3642. pubmed. citation

Knight, K.L. & Sauer, R.T. (1989). Identification of Functionally Important Residues in the DNA-binding Region of the Mnt Repressor. J. Biol. Chem. 264, 13706-13710. pubmed. citation

Knight, K.L. & Sauer, R.T. (1989). DNA Binding Specificity of the Arc and Mnt Repressors Is Determined By a Short Region of N-terminal Residues. Proc. Natl. Acad. Sci. U.S.A. 86, 797-801. pubmed. citation

Knight, K.L. and Sauer, R.T. (1988). The Mnt Repressor of Bacteriophage P22: Role of C-terminal Residues in Operator Binding and Tetramer Formation. Biochemistry 27, 2088-2094. pubmed. citation

RICH BREYER

Richard Breyer
Graduate Student (1985-1989)
Email (rich.breyer at vanderbilt.edu)

Faculty
Vanderbilt University

Reidhaar-Olson, J.F., Bowie, J.U., Breyer, R.M., Hu, J.C., Knight, K.L., Lim, W.A., Mossing, M.C., Parsell, D.A., Shoemaker, K.R.& Sauer, R.T. (1991). Random Mutagenesis of Protein Sequences Using Oligonucleotide Cassettes. in Methods in Enzymology vol 208 (Sauer, R.T., ed.) pps. 564-586, Academic Press, Orlando, FL. pubmed citation

Breyer, R.M. & Sauer, R.T. (1989). Mutational Analysis of the Fine Specificity of Binding of Monoclonal Antibody 51F to l Repressor. J. Biol. Chem. 264, 13355-13360. pubmed citation

Breyer, R.M. & Sauer, R.T. (1989). Production and Characterization of Monoclonal Antibodies to the N-terminal Domain of l Repressor. J. Biol. Chem. 264, 13348-13354. pubmed citation

Reilly, E.B., Reilly, R.M., Breyer, R.M., Sauer, R.T. and Eisen, H.N. (1984). Amino Acid and Nucleotide Sequences of Variable Regions of Mouse Immunoglobulin Light Chains of the l3 Subtype. J. Immunol. 133, 471-475. pubmed citation

Breyer, R.M., Sauer, R.T. and Eisen, H.N. (1981). The Variable Region of Mouse l3 Chains. In: Immunoglobulin Idiotypes (C.A. Janeway, E.E. Sercarz, H. Wigzell and C.F. Fox, eds.) ICN-UCLA Symposia on Molecular and Cellular Biology, Academic Press, NY, pps. 105-110. pubmed citation

ANDREW PAKULA

Andrew Pakula
Graduate Student (1982-1988)
Email (apakula at gmail.com)

Pakula, A.A. & Sauer, R.T. (1990). Reverse Hydrophobic Effects Relieved by Amino-Acid Substitutions at a Protein Surface. Nature 344, 363-364. pubmed. citation

Pakula, A.A. & Sauer, R.T. (1989). Genetic Analysis of Protein Stability and Function. Annual Review of Genetics 23, 289-310. pubmed. citation

Pakula, A.A. & Sauer, R.T. (1989). Amino Acid Substitutions that Increase the Thermal Stability of the l Cro Protein. Proteins: Structure, Function & Genetics 5, 202-210. pubmed. citation

Sauer, R.T., Hecht, M.H., Nelson, H.C.M. and Pakula, A. (1987). Identifying the Determinants of Protein Function and Stability. In: New Frontiers in the Study of Gene Functions (G. Poste and S. Crooke, eds.), Plenum, pps. 177-198.

Pakula, A., Young, V. and Sauer, R.T. (1986). l Cro Mutations: Effects on Activity and Intracellular Degradation. Proc. Nat. Acad. Sci. U.S.A. 83, 8829-8833. pubmed. citation

ANDREW VERSHON

Andrew Vershon
Graduate Student (1982-1986)
Email (vershon at waksman.rutgers.edu)

Faculty
Rutgers, the State University of New Jersey

Zagorski, M.G., Bowie, J.U., Vershon, A.K., Sauer, R.T. & Patel, D.J. (1989) NMR Studies of the Arc Repressor Mutant PL8: Resonance Assignments, Secondary Structure and Long Range Contacts. Biochemistry 28, 9813-9825. pubmed. citation

Knight, K.L., Bowie, J.U., Vershon, A.K., Kelley, R.D. & Sauer, R.T. (1989). The Arc and Mnt Repressors: A New Class of Sequence Specific DNA-Binding Protein. J. Biol. Chem. 264, 3639-3642. pubmed. citation

Vershon, A.K., Kelley, R.D. & Sauer, R.T. (1989) Sequence-specific Binding of Arc Repressor to DNA: Effects of Operator Mutations and Modifications. J. Biol. Chem. 264, 3267-3273. pubmed. citation

Vershon, A.K., Liao, S-M., McClure, W.R. and Sauer, R.T. (1987). Bacteriophage P22 Mnt Repressor: DNA Binding and Effects on Transcription in Vitro. J. Mol. Biol. 195, 311-322. pubmed. citation

Vershon, A.K., Liao, S-M., McClure, W.R. and Sauer, R.T. (1987). Interaction of the Bacteriophage P22 Arc Repressor with Operator DNA. J. Mol. Biol. 195, 323-331. pubmed. citation

Vershon, A.K., Bowie, J.U., Karplus, T.M. and Sauer, R.T. (1986). Isolation and Analysis of Arc Repressor Mutants: Evidence for an Unusual Mechanism of DNA Binding. Proteins: Structure, Function & Genetics 1, 302-311. pubmed. citation

Vershon, A.K., Blackmer, K. and Sauer, R.T. (1986). Mutagenesis of the Arc Repressor Using Synthetic Primers with Random Nucleotide Substitutions. In: Protein Engineering (M. Inouye and R. Sarma, eds.), Academic Press, Orlando, Florida, pps. 243-256.

Jordan, S.R., Pabo, C.O., Vershon, A.K. and Sauer, R.T. (1985). Crystallization of the Arc Repressor. J. Mol. Biol. 185, 445-446.

Vershon, A.K., Youderian, P., Weiss, M.A., Susskind, M.M. and Sauer, R.T. (1985). Mnt Repressor-Operator Interactions: Altered Specificity Requires N6 Methylation of Operator DNA. In: Specificity in Transcription and Translation (R. Calendar and L. Gold, eds.), Alan Liss, Inc., New York, NY, pps. 209-218.

Vershon, A.K., Youderian, P., Susskind, M.M. and Sauer, R.T. (1985). The Bacteriophage P22 Arc and Mnt Repressors: Overproduction, Purification, and Properties. J. Biol. Chem. 260, 12124-12129.

Youderian, P., Vershon, A., Bouvier, S., Sauer, R.T. and Susskind, M.M. (1983). Changing the DNA-Binding Specificity of a Repressor. Cell 35, 777-783. pubmed. citation

FRED GIMBLE

Fred Gimble
Graduate Student (1981-1986)
Email (fgimble at purdue.edu)

Faculty
Purdue University

Gimble, F.S. & Sauer, R.T. (1989). Lambda Repressor Mutants that are Better Substrates for RecA-mediated Cleavage. J. Mol. Biol. 206, 29-39. pubmed. citation

Gimble, F.S. and Sauer, R.T. (1986). Lambda Repressor Inactivation: Properties of Purified Ind- Proteins in the Autodigestion and RecA-mediated Cleavage Reactions. J. Mol. Biol. 192, 39-47. pubmed. citation

Gimble, F.S. and Sauer, R.T. (1985). Mutations in Bacteriophage lambda Repressor that Prevent RecA-Mediated Cleavage. J. Bacteriol. 162, 147-154. pubmed. citation

Sauer, R.T., Nelson, H.C.M., Hehir, K., Hecht, M.H., Gimble, F.S., DeAnda, J. and Poteete, A.R. (1983). The lambda and P22 Phage Repressors. J. Biomol. Structure and Dynamics 1, 1011-1022. pubmed. citation

HILLARY NELSON

Hillary Nelson
Graduate Student (1980-1985)
Email (hnelson at mail.med.upenn.edu)

Faculty
University of Pennsylvania School of Medicine

Sauer, R.T., Hecht, M.H., Nelson, H.C.M. and Pakula, A. (1987). Identifying the Determinants of Protein Function and Stability. In: New Frontiers in the Study of Gene Functions (G. Poste and S. Crooke, eds.), Plenum, pps. 177-198. pubmed. citation

Nelson, H.C.M. and Sauer, R.T. (1986). Interaction of Mutant lambda Repressors with Operator and Non-Operator DNA. J. Mol. Biol. 192, 27-38. pubmed. citation

Nelson, H.C.M. and Sauer, R.T. (1985). Lambda Repressor Mutations that Increase the Affinity and Specificity of Operator Binding. Cell 42, 549-558. pubmed. citation

Hecht, M.H., Hehir, K.M., Nelson, H.C.M., Sturtevant, J.M. and Sauer, R.T. (1985). Increasing and Decreasing Protein Stability: Effects of Revertant Substitutions on the Thermal Denaturation of Phage lambda Repressor. J. Cellular Biochem. 29, 217-224. pubmed. citation

Sauer, R.T., Nelson, H.C.M., Hehir, K., Hecht, M.H., Gimble, F.S., DeAnda, J. and Poteete, A.R. (1983). The lambda and P22 Phage Repressors. J. Biomol. Structure and Dynamics 1, 1011-1022. pubmed. citation

Hecht, M.H., Nelson, H.C.M. and Sauer, R.T. (1983). Mutations in lambda Repressor's Amino-Terminal Domain: Implications for Protein Stability and DNA Binding. Proc. Nat. Acad. Sci. U.S.A. 80, 2676-2680. pubmed. citation

Nelson, H.C.M., Hecht, M.H. and Sauer, R.T. (1983). Mutations Defining the Operator Binding Sites of Bacteriophage lambda Repressor. Cold Spring Harbor Symposium 47, 441-449. pubmed. citation

MICHAEL HECHT

Michael Hecht
Graduate Student (1980-1985)
Email (hecht at princeton.edu)

Faculty
Princeton University

Sauer, R.T., Hecht, M.H., Nelson, H.C.M. and Pakula, A. (1987). Identifying the Determinants of Protein Function and Stability. In: New Frontiers in the Study of Gene Functions (G. Poste and S. Crooke, eds.), Plenum, pps. 177-198.

Hecht, M.H., Sturtevant, J.M. and Sauer, R.T. (1986). Stabilization of l Repressor Against Thermal Denaturation by Site-Directed GlyÆAla Changes in a-Helix Three. Proteins: Structure, Function and Genetics 1, 43-46. pubmed citation

Hecht, M.H., Hehir, K.M., Nelson, H.C.M., Sturtevant, J.M. and Sauer, R.T. (1985). Increasing and Decreasing Protein Stability: Effects of Revertant Substitutions on the Thermal Denaturation of Phage lambda Repressor. J. Cellular Biochem. 29, 217-224. pubmed. citation

Hecht, M.H. and Sauer, R.T. (1985). Phage lambda Repressor Revertants: Amino Acid Substitutions that Restore Activity to Mutant Proteins. J. Mol. Biol. 186, 53-63. pubmed. citation

Hecht, M.H., Sturtevant, J.M. and Sauer, R.T. (1984). Effect of Single Amino Acid Replacements on the Thermal Stability of the NH2-Terminal Domain of Phage l Repressor. Proc. Nat. Acad. Sci. U.S.A. 81, 5685-5689. pubmed. citation

Sauer, R.T., Nelson, H.C.M., Hehir, K., Hecht, M.H., Gimble, F.S., DeAnda, J. and Poteete, A.R. (1983). The lambda and P22 Phage Repressors. J. Biomol. Structure and Dynamics 1, 1011-1022.

Hecht, M.H., Nelson, H.C.M. and Sauer, R.T. (1983). Mutations in lambda Repressor's Amino-Terminal Domain: Implications for Protein Stability and DNA Binding. Proc. Nat. Acad. Sci. U.S.A. 80, 2676-2680.

Nelson, H.C.M., Hecht, M.H. and Sauer, R.T. (1983). Mutations Defining the Operator Binding Sites of Bacteriophage lambda Repressor. Cold Spring Harbor Symposium 47, 441-449.

JAIME DE ANDA

Jaime De Anda
Graduate Student (1979-1985)
Email (Jaime_DeAnda at icoc.org)

President and Chairman, MCA Missions; Irvine, CA (www.mcamissions.org)

President, Science Innovation Fund LLC; Irvine, CA (www.scienceinnovationfund.com)

Adjunct Professor of Chemistry, Cypress College, CA (jdeanda@cypresscollege.edu)

International Church of Christ

Sauer, R.T., Nelson, H.C.M., Hehir, K., Hecht, M.H., Gimble, F.S., DeAnda, J. and Poteete, A.R. (1983). The lambda and P22 Phage Repressors. J. Biomol. Structure and Dynamics 1, 1011-1022.

DeAnda, J., Poteete, A.R. and Sauer, R.T. (1983). P22 c2 Repressor: Domain Structure and Function. J. Biol. Chem. 258, 10536-10542.

Sauer, R.T., Krovatin, W., DeAnda, J., Youderian, P. and Susskind, M. (1983). Primary Structure of the immI Immunity Region of Bacteriophage P22. J. Mol. Biol. 168, 699-713.