SAUER LAB

BIOLOGY DEPARTMENT
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ROBERT T. SAUER

RTSauer

 

 

Bob Sauer
Salvador E. Luria Professor of Biology
Principal Investigator (1978 - Present)

Email (bobsauer [at] mit.edu
)

Current C.V.

Kresge, N., Simoni, R., and Hill, R. (2009)
A Model for Protein Turnover: the Work of
Robert T. Sauer
J Biol Chem 284(49): e22-e23, PMC2797215.

Bob Sauer's Biology Webpage

 

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Professor Sauer teaches:

Graduate Biochemistry (7.51)

 

 

ROBERT A. GRANT

ROBERTGRANT

 

Robert A.Grant
Research Scientist (2000 - Present)

Email (ragrant [at] mit.edu
)

BGrant

Stein, B.J., Grant, R.A., Sauer, R.T., & Baker, T.A. (2016) Structural basis of an N-degron adaptor with more stringent specificityStructure 2, 232-242.

Román-Hernández G, Hou JY, Grant RA, Sauer RT, Baker TA.(2011) The ClpS adaptor mediates staged delivery of N-end rule substrates to the AAA+ ClpAP protease. Mol Cell. Jul 22;43(2):217-28. PubMed PMID: 21777811; PubMed Central PMCID: PMC3168947.

Kim S, Grant RA, Sauer RT. Covalent linkage of distinct substrate degrons controls assembly and disassembly of DegP proteolytic cages. Cell. 2011 Apr 1;145(1):67-78. PubMed PMID: 21458668; PubMed Central PMCID: PMC3075617.

Wang, K.H., Román-Hernández, G, Grant, R.A., Sauer, R.T. & Baker, T.A. (2008) The molecular basis of N-end rule recognition. Mol. Cell 32(3):406-14. PubMed PMID: 18995838; PubMed Central PMCID: PMC3114436 [Indexed for MEDLINE]

Chein, P., Grant, R.A., Sauer, R.T. & Baker, T.A. (2007) Structure and substrate specificity of an SspB ortholog: design implications for AAA+ adaptors. Structure 15, 139-145.

Sohn, J., Grant, R.A. & Sauer, R.T. (2007) Allosteric activation of DegS, a stress sensor PDZ-protease. Cell 131, 572-583.

Bolon, D.N., Grant, R.A., Baker, T.A. & Sauer, R.T. (2005) Specificity versus stability in computational protein design. Proc. Natl. Acad. Sci. USA 102, 12724-12729. pubmed. citation

 

KARL SCHMITZ

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Karl Schmitz
Postdoc Associate (2010 -June 2015)
and
Postdoc Fellow (July 2015-Present)
The Medical Foundation
Email (schmitzk [at] mit.edu)

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Alvaro, A.J., Schmitz, K.R., Sello, J.K., Baker, T.A. & Sauer, R.T. (2016) Highly dynamic interactions maintain kinetic stability of the ClpXP protease during the ATP-fueled mechanical cycle. ACS Chem. Biol. 30 March 2016.

Schmitz, K.R. and Sauer, R.T. (2014) Substrate delivery by the AAA+ ClpX and ClpC1 unfoldases activates the mycobacterial ClpP1P2 peptidase Mol. Microbiology 93(4):617-28. doi: 10.1111/mmi.12694. Epub 2014 Jul 13.

Carney, D., Schmitz, K.R., Truong, J., Sauer, R.T., and Sello, J.K. (2014) Restriction of the conformational dynamics of the cyclic acyldepsipeptide macrocycle improves antibacterial activity by enhancing both ClpP peptidase binding and activation. J. Amer. Chem. Soc. 136, 1922-1929.

Carney, D.W., Compton, C.L., Schmitz, K.R., Stevens, J.P., Sauer, R.T. & Jason K. Sello (2014) A simple fragment of the acyldepsipeptide antibacterial agents is necessary and sufficient for ClpP activation and antibacterial activity. Chembiochem. 15(15):2216-20. doi: 10.1111/mmi.12694. Epub 2014 Jul 13.

Stinson, B.M., Nager, A.R., Glynn, S.E., Schmitz, K.R., Baker, T.A. & Sauer, R.T. (2013) Nucleotide Binding and Conformational Switching in the Hexameric Ring of a AAA+ Machine. Cell 153(3): 628-39.

 

OHAD YOSEFSON

OhadYosefson

 

 

Ohad Yosefson
Postdoc Associate (October 2011-Present)

Email (ohady [at] mit.edu)

 

OhadImage

 

 

 

Iosefson, O., Olivares, A.O., Baker, T.A. & Sauer, R.T. (2015) Dissection of Axial-Pore Loop Function during Unfolding and
Translocation by a AAA+ Proteolytic Machine
.
Cell Reports 11; 12(6), 1032-41. doi: 10.1016/j.celrep.2015.07.007.

Iosefson, O., Nager, A.R., Baker, T.A. & Sauer, R.T. (2015) Coordinated gripping of substrate by subunits of a AAA+ proteolytic machine Nat. Chem. Biol. 11(3):201-6. doi: 10.1038/nchembio.1732.

Olivares, A.O., Nager, A.R., Iosefson, O., Sauer, R.T. & Baker, T.A. (2014) Mechanochemical basis of protein degradation by a double-ring AAA+ machine Nat. Struct. Mol. Biol. 21, 871-875.

 

VLADIMIR BAYTSHTOK

Vlad

 

Vladimir Baytshtok
Graduate Student (June 2011 - Present)

Email (vbaytsht [at] mit.edu)

 

Baytshtok, V., Baker, T.A. & Sauer, R.T. (2015) Assaying the kinetics of protein denaturation catalyzed by AAA+ unfolding machines and proteases. Proc. Natl. Acad. Sci. USA 112, 5377-5382. pubmed. citation

 

 

 

 

 

 

ALVARO "AJ" AMOR

A_Amon

 

Alvaro "AJ" Amor
Graduate Student (June 2013-Present)

Email (alamor [at] mit.edu)

 

 

Alvaro, A.J., Schmitz, K.R., Sello, J.K., Baker, T.A. & Sauer, R.T. (2016) Highly dynamic interactions maintain kinetic stability of the ClpXP protease during the ATP-fueled mechanical cycle. ACS Chem. Biol. 30 March 2016

 


SANJAY HARI

Hari

 

Sanjay Hari
Postdoc Associate (2014-August 2015)
and
NIH Postdoc Fellow (September 2015-Present)
Email (sanjayh [at] mit.edu)

 

 

XUE FEI

XueFei Image

 

Xue Fei
Postdoc Associate (February 2015-Present)

Email (xfei [at] mit.edu)

 

ADRIAN OLIVARES

AOLIVARES

 

Adrian Olivares
Research Scientist (July 2015-Present)

Email (olivares [at] mit.edu)

 

AO RESEARCH IMAGE

Olivares, A.O., Baker, T.A., and Sauer, R.T. (2016) Mechanistic insights into bacterial AAA+ proteases and protein-remodeling machines. Nat. Rev. Microbiol 14(1):33-44.

Iosefson, O., Olivares, A.O., Baker, T.A., and Sauer, R.T. (2015) Dissection of axial-pore loop function during unfolding and translocation by a AAA+ proteolytic machine. Cell Reports 12(6):1032-1041, PMC4536184.

Olivares, A.O., Nager, A.R., Iosefson, O., Sauer, R.T., and Baker, T.A. (2014) Mechanochemical basis of protein degradation by a double-ring AAA+ machine. Nat. Struct. Mol. Biol. 21(10):871-875, PMC4190165.

Cordova, J.C.*, Olivares, A.O.*, Shin, Y.*, Stinson, B.M., Calmat, S., Schmitz, K.R., Aubin-Tam, M.E., Baker, T.A., Lang, M.J., and Sauer, R.T. (2014) Stochastic but highly coordinated protein unfolding and polypeptide translocation by the ClpXP proteolytic machine. Cell 158(3):647-658, PMC4134808. *equal contribution

Aubin-Tam, M.E.*, Olivares, A.O.*, Sauer, R.T., Baker, T.A., and Lang, M.J. (2011) Single-molecule protein unfolding and translocation by an ATP-fueled proteolytic machine. Cell 145(2):257-267, PMC3108460. *equal contribution


REUBEN SAUNDERS

ReubenSauders'sImage

Reuben Saunders
UROP (February 2014-June 2016)
Summer Research Assistant (June 2016-Present)
Email (reubens [at] mit.edu)

Hertz Fellowship


 

 

ERIN DEVINE

ErinDevine

 

Erin Devine
Postdoc Associate (February 2016 - Present)

Email (edevine [at] mit.edu)

 

TRISTAN BELL

TristanBell

 

Tristan Bell
Graduate Student (June 2016-Present)

Email (tribell [at] mit.edu)

 

JUHEE PARK

JuheePark

 

Juhee Park
Graduate Student (June 2016-Present)

Email (jpark05 [at] mit.edu)

 

BRENDA ELLEN PEPE

Brenda Pepe

Brenda Ellen Pepe
Administrative Assistant, Sauer Lab (pepebe [at] mit.edu)

I am a proud Boston College alumnus, class of 1985 (WCAS) and 1989 (GA&S). I live on the South Shore and in my free time I enjoy gardening, reading,worldwide travel, and genealogy research.

 


 

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Sauer Lab, Massachusetts Institute of Technology
77 Massachusetts Avenue, 68-571
Cambridge, MA 02139
Telephone: 617.253.6077, Fax: 617.258.0673

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