Massachusetts Institute of Technology Massachusetts Institute of Technology
Department of Chemical Engineering


Hadley D. Sikes Lab : Publications


Peer-reviewed publications

Joseph B. Lim, Kimberly A. Barker, Linda Jiang, Veronica Molina, Jessica Saifee, Hadley D. Sikes. "Insights into electron leakage in the reaction cycle of cytochrome P450 BM3 revealed by kinetic modeling and mutagenesis," in revision.

Brandon W. Heimer and Hadley D. Sikes. "Characterization and Directed E volution of a Methyl Binding Domain Protein for High-Sensitivity DNA Methylation Analysis," in revision.

Beijing K. Huang, Sohail Ali and Hadley D. Sikes. "Interpreting heterogeneity in the response of cells expressing a fluorescent biosensor to stimulation with hydrogen peroxide," in revision.

Amy Reece, Kaja Kaastrup, Hadley D. Sikes and John Oakey. "Staged Inertial Microfluidic Focusing for Complex Fluid Enrichment," RSC Advances, 2015. DOI: 10.1039/C5RA10634F.

Joseph B. Lim, Beijing K. Huang, William M. Deen and Hadley D. Sikes “Analysis of the lifetime and spatial localization of hydrogen peroxide generated in the cytosol using a reduced kinetic model,” in revision.

Jisam Wong, Kaja Kaastrup, Alan Aguirre-Soto and Hadley D. Sikes. "A quantitative analysis of peroxy- mediated cyclic regeneration of eosin in oxygen-rich photopolymerization conditions," Polymer, 2015. doi:10.1016/j.polymer.2015.05.043

Eric Miller and Hadley D. Sikes, "Addressing barriers to the development and adoption of rapid diagnostic tests in global health," Nanobiomedicine, 2015.

Kaja Kaastrup and Hadley D. Sikes, "Investigation of dendrimers functionalized with eosin as macrophotoinitiators for polymerization-based signal amplification reactions," RSC Adv., 2015, 5, 15652-15659 DOI: 10.1039/C4RA14466J. 

Joseph B. Lim and Hadley D. Sikes, "Use of a genetically encoded hydrogen peroxide sensor for whole cell screening of enzyme activity," Protein Engineering, Design and Selection, (2015) 28 (3):79-83doi:10.1093/protein/gzv003.

Hadley D. Sikes and Nicola Zamboni, "Analytical Biotechnology," Current Opinion in Biotechnology, 2015, 31:iv–vi.


Abraham K. Badu-Tawiah,* Shefali Lathwal,* Kaja Kaastrup, Mohammad Al-Sayah, Dionysios C. Christodouleas, Barbara S. Smith, George M. Whitesides,* and Hadley D. Sikes,* "Polymerization-based signal amplification for paper-based immunoassays" Lab on a Chip, 2015, DOI:10.1039/C4LC01239A

J. B. Lim, K. A. Barker, B. K. Huang, H. D. Sikes "In-depth characterization of the fluorescent signal of HyPer, a probe for hydrogen peroxide, in bacteria exposed to external oxidative stress" Journal of Microbiological Methods, 2014, 106, 33-39.

B.K. Huang and H.D. Sikes "Quantifying intracellular hydrogen peroxide perturbations in terms of concentration" Redox Biology, 2014, DOI: 10.1016/j.redox.2014.08.001.

B.W. Heimer, T.A. Shatova, J.K. Lee, K. Kaastrup and H.D. Sikes "Evaluating the Sensitivity of Hybridization-Based Epigenotyping using a Methyl Binding Domain Protein" Analyst, 2014, 139,3695-3701.

J.K. Lee and H.D. Sikes "Balancing the initiation and molecular recognition capabilities of eosin macroinitiators of polymerization-based signal amplification reactions" Macromolecular Rapid Communications, 2014, DOI: 10.1002/marc.201400035.

K. Kaastrup, L. Chan, H.D. Sikes "Impact of dissociation constant on detection sensitivity of polymerization-based signal amplification reactions" Analytical Chemistry, 2013, 85: 17, 80558060.

K. Kaastrup and H.D. Sikes "Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times" Lab on a Chip, 2012, DOI: 10.1039/C2LC40584A.



J.K. Lee, B.W. Heimer, H.D. Sikes. “Systematic study of fluorescein-functionalized macrophotoinitiators for colorimetric bioassays,” Biomacromolecules, 2012, 13: 4, 1136.



M.E. Boyd, B.W. Heimer, H.D. Sikes. “Functional heterologous expression and purification of a mammalian methyl-CpG binding domain in suitable yield for DNA methylation profiling assays,” Protein Expression & Purification, 2012, 82: 2, 332.


H.D. Sikes, R. Jenison, C.N. Bowman. “Antigen detection using polymerization-based amplification,” Lab on a Chip, 2009 5: 653-656. (Featured on the cover; highlighted in RSC Chemical Biology —Research News 2, 2009)

R.R. Hansen, H.D. Sikes, C.N. Bowman. “Sub-attomole detection of labeled oligonucleotides using visible light photopolymerization for signal amplification on glass microarray surfacesBiomacromolecules 2008 9: 355-362.

H.D. Sikes, R.R. Hansen, L.M. Johnson, R. Jenison, J.W. Birks, K.L. Rowlen,  and C.N. Bowman. “Using polymeric materials to generate an amplified response to molecular recognition eventsNature Materials 2008 7: 52-56.   (Highlighted in Science 2007 318:5852, 888, in C&EN 2007 85:46, 45, and in Trends in Biotechnology, 2008 26: 11, 581-583.)

J. F. Smalley, S. B.Sachs, C.E.D. Chidsey, S. P. Dudek, H. D. Sikes, S. E. Creager, C. J. Yu, S. W. Feldberg, M.D. Newton. “Interfacial electron-transfer kinetics of ferrocene through oligophenyleneethynylene bridges attached to gold electrodes as constituents of self-assembled monolayers: Observation of a nonmonotonic distance dependenceJ. Am. Chem. Soc. 2004 126: 5, 14620-14630. 

H.D. Sikes, Y. Sun, S.P. Dudek, C.E.D. Chidsey, and P. Pianetta.  “Photoelectron spectroscopy to probe the mechanism of electron transfer through oligophenylenevinylene bridgesJ. Phys. Chem. B  2003 107: 5, 1170-1173.

S.P. Dudek, H.D. Sikes, C.E.D. Chidsey.  “Synthesis of ferrocenethiols containing oligo (phenylenevinylene) bridges and their characterization on gold electrodesJ. Am. Chem. Soc.  2001 123: 8033-8038.

H.D. Sikes, J.F. Smalley, S.P. Dudek, A.R. Cook, M.D. Newton, C.E.D. Chidsey, S.W. Feldberg. “Rapid electron tunneling through oligophenylenevinylene bridgesScience 2001 291: 1519-1523.

H.D. Sikes, D.K. Schwartz. “Two-dimensional melting of an anisotropic crystal observed at the molecular level” Science1997 278: 1604-1607.

H.D. Sikes, D.K. Schwartz. “A temperature-dependent two-dimensional condensation transition during Langmuir-Blodgett depositionLangmuir 1997 13: 4704-4709.

J.T. Woodward, I. Doudevski, H.D. Sikes, D.K. Schwartz. “Kinetics of self-assembled monolayer growth explored via submonolayer coverage of incomplete filmsJ. Phys. Chem. B 1997 101:38, 7535-7541.

H.D. Sikes, J.T. Woodward, D.K. Schwartz. “Pattern formation in a substrate-induced phase transition during Langmuir-Blodgett transferJ. Phys. Chem. 1996 100: 21, 9093-9097.


Patents and patents pending

“Paper-based immunoassay with polymerization-based signal amplification”
Provisional Patent Application filed 11/2013 with Abraham K. Badu-Tawiah, Shefali Lathwal, Mohammad H. Al-Sayah, Christodouleas Dionysios, and George M. Whitesides
8,652,778  “Photopolymerization for amplification and detection of a molecular recognition event” Provisional Patent Application #60/988,563 filed 11/16/2007 with C.N. Bowman, K. Rowlen, R. Hansen, H.J. Avens, Regularized Application #12/099,560 filed 4/8/2008, examined and granted in the US 2/18/2014.
7,354,706  “Photopolymerization for amplification and detection of a molecular recognition event” Provisional Patent Application # 60/501755 filed 9/09/03 with K. Rowlen, J. Birks, C.N. Bowman. Regularized Patent Application #PCT/US2004/029733 filed 9/09/04.  Examined and granted in the US 4/08/08 and in the EU 2/10. Licensed, 2010; products brought to market, 2011.

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