Massachusetts Institute of Technology Massachusetts Institute of Technology
Department of Chemical Engineering


Hadley D. Sikes Lab : Publications


Peer-reviewed publications

Kaja Kaastrup and Hadley D. Sikes, "Investigation of dendrimers functionalized with eosin as macrophotoinitiators for polymerization-based signal amplification reactions," RSC Adv., 2015, 5, 15652-15659 DOI: 10.1039/C4RA14466J. 

Joseph B. Lim and Hadley D. Sikes, "Use of a genetically encoded hydrogen peroxide sensor for whole cell screening of enzyme activity," Protein Engineering, Design and Selection, (2015) 28 (3):79-83doi:10.1093/protein/gzv003.

Hadley D. Sikes and Nicola Zamboni, "Analytical Biotechnology," Current Opinion in Biotechnology, 2015, 31:iv–vi.



Abraham K. Badu-Tawiah,* Shefali Lathwal,* Kaja Kaastrup, Mohammad Al-Sayah, Dionysios C. Christodouleas, Barbara S. Smith, George M. Whitesides,* and Hadley D. Sikes,* "Polymerization-based signal amplification for paper-based immunoassays" Lab on a Chip, 2015, DOI: 10.1039/C4LC01239A

J. B. Lim, K. A. Barker, B. K. Huang, H. D. Sikes "In-depth characterization of the fluorescent signal of HyPer, a probe for hydrogen peroxide, in bacteria exposed to external oxidative stress" Journal of Microbiological Methods, 2014, 106, 33-39.

B.K. Huang and H.D. Sikes "Quantifying intracellular hydrogen peroxide perturbations in terms of concentration" Redox Biology, 2014, DOI: 10.1016/j.redox.2014.08.001.

B.W. Heimer, T.A. Shatova, J.K. Lee, K. Kaastrup and H.D. Sikes "Evaluating the Sensitivity of Hybridization-Based Epigenotyping using a Methyl Binding Domain Protein" Analyst, 2014, 139, 3695-3701.

J.K. Lee and H.D. Sikes "Balancing the initiation and molecular recognition capabilities of eosin macroinitiators of polymerization-based signal amplification reactions" Macromolecular Rapid Communications, 2014, DOI: 10.1002/marc.201400035.

K. Kaastrup, L. Chan, H.D. Sikes "Impact of dissociation constant on detection sensitivity of polymerization-based signal amplification reactions" Analytical Chemistry, 2013, 85: 17, 80558060.

K. Kaastrup and H.D. Sikes "Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times" Lab on a Chip, 2012, DOI: 10.1039/C2LC40584A.



J.K. Lee, B.W. Heimer, H.D. Sikes. “Systematic study of fluorescein-functionalized macrophotoinitiators for colorimetric bioassays,” Biomacromolecules, 2012, 13: 4, 1136.



M.E. Boyd, B.W. Heimer, H.D. Sikes. “Functional heterologous expression and purification of a mammalian methyl-CpG binding domain in suitable yield for DNA methylation profiling assays,” Protein Expression & Purification, 2012, 82: 2, 332.


H.D. Sikes, R. Jenison, C.N. Bowman. “Antigen detection using polymerization-based amplification,” Lab on a Chip, 2009 5: 653-656. (Featured on the cover; highlighted in RSC Chemical Biology —Research News 2, 2009)

R.R. Hansen, H.D. Sikes, C.N. Bowman. “Sub-attomole detection of labeled oligonucleotides using visible light photopolymerization for signal amplification on glass microarray surfacesBiomacromolecules 2008 9: 355-362.

H.D. Sikes, R.R. Hansen, L.M. Johnson, R. Jenison, J.W. Birks, K.L. Rowlen,  and C.N. Bowman. “Using polymeric materials to generate an amplified response to molecular recognition eventsNature Materials 2008 7: 52-56.   (Highlighted in Science 2007 318:5852, 888, in C&EN 2007 85:46, 45, and in Trends in Biotechnology, 2008 26: 11, 581-583.)

J. F. Smalley, S. B.Sachs, C.E.D. Chidsey, S. P. Dudek, H. D. Sikes, S. E. Creager, C. J. Yu, S. W. Feldberg, M.D. Newton. “Interfacial electron-transfer kinetics of ferrocene through oligophenyleneethynylene bridges attached to gold electrodes as constituents of self-assembled monolayers: Observation of a nonmonotonic distance dependenceJ. Am. Chem. Soc. 2004 126: 5, 14620-14630. 

H.D. Sikes, Y. Sun, S.P. Dudek, C.E.D. Chidsey, and P. Pianetta.  “Photoelectron spectroscopy to probe the mechanism of electron transfer through oligophenylenevinylene bridgesJ. Phys. Chem. B  2003 107: 5, 1170-1173.

S.P. Dudek, H.D. Sikes, C.E.D. Chidsey.  “Synthesis of ferrocenethiols containing oligo (phenylenevinylene) bridges and their characterization on gold electrodesJ. Am. Chem. Soc.  2001 123: 8033-8038.

H.D. Sikes, J.F. Smalley, S.P. Dudek, A.R. Cook, M.D. Newton, C.E.D. Chidsey, S.W. Feldberg. “Rapid electron tunneling through oligophenylenevinylene bridgesScience 2001 291: 1519-1523.

H.D. Sikes, D.K. Schwartz. “Two-dimensional melting of an anisotropic crystal observed at the molecular level” Science1997 278: 1604-1607.

H.D. Sikes, D.K. Schwartz. “A temperature-dependent two-dimensional condensation transition during Langmuir-Blodgett depositionLangmuir 1997 13: 4704-4709.

J.T. Woodward, I. Doudevski, H.D. Sikes, D.K. Schwartz. “Kinetics of self-assembled monolayer growth explored via submonolayer coverage of incomplete filmsJ. Phys. Chem. B 1997 101:38, 7535-7541.

H.D. Sikes, J.T. Woodward, D.K. Schwartz. “Pattern formation in a substrate-induced phase transition during Langmuir-Blodgett transferJ. Phys. Chem. 1996 100: 21, 9093-9097.


Patents and patents pending

“Paper-based immunoassay with polymerization-based signal amplification”
Provisional Patent Application filed 11/2013 with Abraham K. Badu-Tawiah, Shefali Lathwal, Mohammad H. Al-Sayah, Christodouleas Dionysios, and George M. Whitesides
8,652,778  “Photopolymerization for amplification and detection of a molecular recognition event” Provisional Patent Application #60/988,563 filed 11/16/2007 with C.N. Bowman, K. Rowlen, R. Hansen, H.J. Avens, Regularized Application #12/099,560 filed 4/8/2008, examined and granted in the US 2/18/2014.
7,354,706  “Photopolymerization for amplification and detection of a molecular recognition event” Provisional Patent Application # 60/501755 filed 9/09/03 with K. Rowlen, J. Birks, C.N. Bowman. Regularized Patent Application #PCT/US2004/029733 filed 9/09/04.  Examined and granted in the US 4/08/08 and in the EU 2/10. Licensed, 2010; products brought to market, 2011.

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