DCIF Mass Spectrometer Instrumentation
[Mass Spectroscopy Links
Fourier Transform Mass Spectrometer:
The Facility houses a Bruker
Daltonics APEXIV 4.7 Tesla Fourier Transform Ion
Cyclotron Resonance Mass Spectrometer (FT-ICR-MS), controlled
by a Windows 2000 PC workstation. Ionization sources include electrospray
(ESI), electron impact (EI), and direct analysis in real time (DART).
The spectrometer is equipped with a Agilent auto sampler
to assist in high throughput ESI analysis. The spectrometer
is run exclusively by Li Li our mass spectrometry research specialist.
- Place sample (~ 0.5mg) in a small sample
vial. Solids are preferred but liquids are OK. Do not use a plastic
vial if the samples are only soluble in chloroform or methylene chloride
- Chemistry Department Researchers should
fill out a mass spectrometric analysis request form (download one in .pdf form here) and
submit the samples to Li Li. If your sample is unstable under
room temperature conditions, you may leave the sample on the bottom shelf in
the DCIF's refridgerator or keep the sample in your lab and Li Li will notify you when
the experiment is ready. Researchers may request several scan variations
so if you are unsure, you are encouraged to discuss analysis details
with Li Li.
- The currently installed ion source (EI,
ESI, and DART) and projected usage dates are listed on the white board
at the entrance to the DCIF.
- Please note!! To verify which ionization
source was used for your sample analysis look at the file name on the
bottom of the spectrum. The nomenclature is as follows:
- File name: xxx/data/XX/XXxxxx_ESI,
Source: Electrospray ion source (ESI)
- File name: xxx/data/XX/XXxxxx_EI, Source:
Electron Impact and Chemical Ionization (EI/CI)
- File name: xxx/data/XX/XXxxxx_DART, Source: Direct Analysis in Real Time (DART)
- An FTMS services guideline
can be downloaded here.
- The terms "High resolution",
"Exact mass", "Mass accuracy" and others are used
almost interchangeably. JEOL M.S. has an excellent overview defining
these, and other, mass spectrometry terms. View it here.
- If you would like to import your FTMS spectrum
into a Word document click here!
Gas Chromatograph/Mass Spectrometer:
The DCIF has an Agilent
5973N Gas Chromatograph/Mass Spectrometer (GC/MS) -
This is a student operated unit mass, electron impact GC/MS. This instument
is good for volatile, neutral molecules up to ~500amu.
- A training sheet for the new GC/MS can
be downloaded here.
- After downloading the training sheet, use the Training Request Form to schedule an appointment.
- For nostalgia puposes we have retained
our graphic Hewlett-Packard MS ChemStation Tour. It will only take a
few moments but it will help you become familiar with some of the features
along with the now somewhat outdated Windows 3.1 software interface
before training. Click here
to take the tour.
- A Sample concentration calculator for the
GC-MS is here.
Bruker Omniflex MALDI-TOF:
The DCIF has a Bruker
Omniflex MALDI-TOF instrument with a Reflectron accessory. This instrument
is ideal for unit mass determination and structural analysis of non-volatile organic
compounds (with N, O, S or aromatic rings), bio-molecules, polymers, proteins, and many other molecules
in the ~600 to 60,000 amu mass range (possibly higher). The instrument
is student operated. In order to gain instrument access users must be trained by the
Li Li, the DCIF mass spectroscopist.
The MALDI-TOF training's are divided into two separate sessions.
- The first sessions will focus on sample
preparation and can accommodate up to four (4) students. These sessions
will also address the proper handling / preparation of the MALDI targets
and further address issues concerning the different matrices and calibration
standards. These sessions are critical as proper / careful sample preparation
is of the utmost importance in a MALDI-TOF experiment. The sample preparation
session must be completed before you start the instrument operation
- The second session will concentrate on
instrument function / features / and operation. These sessions can only
accommodate two (2) students.
- The DCIF does not supply targets, matrices,
or calibration standards but does have a few targets that groups may
"check out" until a group acquires their own target(s).
- The MALDI targets are available from Bruker
- Matrices and calibration standards are
available from various sources (including Bruker) and prices may vary
anywhere from ~$200 to $500 depending upon the compounds desired.
- If you would like to be trained, see the Training Request Form to schedule an appointment. .
Get a MALDI training sheet here
and a MALDI sample
preparation sheet here .
Waters Q-TOF Micro Mass Spectrometer Trainings Now Available!
The DCIF has a Waters Q-TOF micro mass spectrometer on open access to researchers. The Q-TOF is equipped with an ESI (electrospray ionization) ion source, a TOF (Time of Flight) detector, secondary mass (MS/MS) utility, and an Agilent 1100 auto-sampler. Q-TOF analysis is suitable for organic polar compounds with molecular weights up to 10,000 daltons. Samples must be soluble in water or an organic solvent such as methanol, acetonitrile, acetone, ethyl acetate, dichloromethane, or chloroform. The Q-TOF is ideal for obtaining unit mass data. Exact mass results require an internal calibration.
See the Training Request Form to schedule an appoinment.
Training sheets and sample prep information will be posted in this space soon.