Why are my 13C peaks split?

This is a good question and there are a number of reasons why your carbon peaks may be split into mutiplets.   Incredibly enough some of these may even fall into the "Something is wrong with the spectrometer.  I know it's not my chemistry." category.   The following list is by no means comprehensive but rather a listing of some of the more common reasons you may see "carbon splitting".   Some of these are specific to the MIT facility but some are not.   These include:

  • Probe tuning (most common)...
  • Incomplete decoupling (can be related to probe tuning)...
  • Low decoupler power output (can be hardware or software related)...
  • Incorrect probe file loaded (software)...
  • Decouple mode (software)...
  • Shims...


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    Now stop crying and get back to work!