- The first thing you may want to look at
is our FAQ (Frequently Asked Questions) sheets. This answers and addresses
many of the little problems we encounter on a day to day basis. You
can view or download the FAQ sheet: (Varian )(Bruker )
- Here is an algorithm
for determining the resonances of a 'known' structure.
- Here is a helpful algorithm
for determining the resonances of an 'unknown' structure.
- Ever wonder 'Which 2D experiment is best
for me?' This useful data sheet may help you in determining which experiment
is best for you. This sheet summarizes some of the more common 2D pulse
sequences including the information content and pros and cons. Get one here !
- Here are some other Academic NMR Facility
tutorial / instruction sheets / user guides. These include numerous
hardware, software, and experimental tips. I've tried to group them
by manufacturer but the is a lot of general information available as
Solvent Reference Lists:
- The NMR Facility at Indiana University
has an extensive list of 19F Chemical Shifts and Coupling Constants.
You can find it here.
- If you prefer your references in .pdf format here is a list from the University of Colorado
- The NMR periodic table has some good information about Fluorine and other NMR active nuclei
- An extensive Phosphorous NMR reference
listing can be found at "The Wired Chemist". The url is here.
- Another can be found at the University
of Indiana. You can get it here
Experiment Guideline Sheets: (click the acronym to get a description of the experiment)
- Instrument training sheets (covering simple
1D 1H and 13C spectra) are available here.
- Locking instructions for solvents with multiple types of 2H (CD3OD).
Instruction Manuals from the University of Minnesota Chemistry Department
NMR Facility. Well thought out and well organized. These are really
Observing Nuclei Other than 1H and 13C :
- Instructions for fluorine and phosphorus acquisition on the Varian 300
- Instructions for running deuterium NMR (Varian 500)
- Here is an overview for doing 2D (COSY, ROESY, TOCSY, NOESY, HMBC, HSQC and HMQC)
on the Varians .
- Instructions to run a TOCSY are here (Varian )
- How to phase a 2D spectrum (Varian).
- How to plot a 2D spectrum (Varian).
- Guidelines to perform a ROESY experiment are here (Varian) (Bruker).
- How to perform a NOESY experiment can be found here (Varian) (Bruker ).
- How to run a COSY on the Brukers can be found here.
- Get your HETCOR instructions here (Varian ) (Bruker ).
- The HMBC instruction
sheet is available here (Varian) (Bruker).
- The HSQC instruction
sheet is available here (Varian )
- The HMQC experiment
is described here (Varian ) (Bruker).
- Quick tips for running a GCOSY (Varians ).
- A more detailed description can be
found here .
- How to calibrate
a 90o pulse (Varian ) (Topspin 1.3 ).
- Bruker VT instructions: Bruker 400 & 401 .
- Simple water suppression experiment using PRESAT (Varian ).
- Water or solvent suppression for the Brukers (Topspin 1.3) (Topspin 3.1)
- Other solvent suppression techniques
include BINOM, JUMPRET,
- Selective suppression (or excitation)
using shaped pulses (Varian )
- How to gradient shim your sample (Varian ) (Bruker 600 ).
- Homospoil gradient shimming the Varian.
- Instructions for increasing the number of scans while the acquisition is running, or adding scans to a completed experiment, are here (Varian )
- Instructions for queueing experiments in Bruker (setting up multiple experiments at once) can be found here
Plotting, Printing & Exporting Tips: (these links
are a mix of .pdf files and pop-up windows)
- Instructions on backing up and transferring data with SecureFx can be found here . SecureFx can be downloaded here.
- How can I export my spectrum into a graphics file?
: postscript .ps files) (Topspin 1.3 :.ps, .pdf, .jpg, .tif & .png files) (Topspin 3.1- use Publish> Save as Pdf)
- Instructions on how to convert your Varian data to the
Bruker format, in order to use Topspin Plot Editor, can be obtained here .
- Instructions on how to download and install MNova Lite (AKA MestRec, AKA MestReNova) on your own computer are here
- Instructions on how to convert your Varian & Bruker data to the
MNova Lite/Mestrec/MestReNova format can be obtained here .
- Instructions on how to plot multiple spectra
on one page can be obtained here. (Varian ) (Bruker)
- Instructions on how to make a white washed
stacked plot can be found here. (Varian ) (Bruker- use instructions above for regular stacked plots)
- How do I make insets? (Varian) (Bruker: Use Topspin Plot Editor program)
- Can I import my spectrum into a spreadsheet
(Excel, Sigmaplot, Origin, etc)? Check these links. (Varian)
Instrument Problems and Curiosities? (most
of these quick tips will open a small pop-up window)
- The colors are flashing in the CDE. What's
- The variable temperature (VT) unit isn't
working. What's wrong? (Varian)
- My sample won't spin. Find out why here.
- What are Pulsed Field Gradients
- Having problems with probe tuning? Check
out this excerpt from the October
14th 2002 edition of Varian VNR News. You'll be tuning like
a pro in no time!
- What is the relaxation
time? What's the difference between T1 and T2?
- How do I unlock an experiment that's locked?
- Something is wrong with the spectrometer.
( I can't lock.)
- Click here to take a graphical tour on how to lock the Varians.
- My sample won't shim. (Varian
- If you would like more information
on shimming here are a few online resources gathered by Adrienne
B. Hoeglund through the AMMRL...
- A paper by Gerald A. Pearson at
the University of Iowa can be found here.
- A nice little tutorial at the University
of Saskatchewan can be found here.
- And the classic "Shimming
Ain't Magic" paper at Acorn NMR Inc. can be found here.
- Which spectrometer should I use for carbon?
- What the heck is apodization?
- How does 'resolution
- What is the Fourier
- What does the "ADC overflow" error message
- All of my 13C signals are split
into multiplets. Click here.
These tips are good for both the Bruker and Varian NMR spectrometers.
- I have integral concerns. What can I do
to "improve" my values? (Varian)
- I need to run my spectra at a higher field
to get better resolution. (Not necessarily
NMR Sample Prep and Tube Care:
- NMR sample preparation tips are here.
- These tips come directly from Wilmad Glass...