LBRC Research Laboratories Guidelines

Main Laboratories and Facilities Equipment

CELL CULTURE FACILITY/CHEMICAL PREPARATION ROOM
(6-010 & 6-012, Ramachandra R. Dasari)

This laboratory is to store and prepare biological/chemical samples.

Centrifuge

1. 5804R (Eppendorf)
2. 5415 (Eppendorf)

Shaker Bath, Model 50 (Precision)

- Capacity: 26.5 L.
- Temperature Range: Ambient +100°C with cover

CO2 incubator, Steri-cycle (Thermo Forma)

- Capacity:  6.5 cu. ft. (184L)
- CO2 Concentration Range: 0 to 20%
- Temperature Range: Ambient +5°C to +50°C
- Relative Humidity:  Ambient to 95% at 37°C

Biological safety cabinet, SterilGARD III Advance (Baker co.)

- Class II Type B2 (exhaust to outside)

Refrigerators

- General purpose, 4°C (Lab Research Product Inc.)
- Frozen cell storage, -80°C (VWR)
- Frozen medium storage, -20°C (VWR)

Microscopes for sample observation

- Axioskop2 plus (Zeiss)
- Axiovert 25 (Zeiss)

Microtome Cryostat, HM525 (Microm)

- Storage temperature: -20°C up to 50°C

UV Curing system, UV75 Light (Thorlabs)

- Light Intensity >40mW

Chemical fume hood (Mott)

Analytical balance (Mettler Toledo)

Ultrasonic cleaner, 1510 (Branson)

- Tank capacity: 0.50 gal
- Frequency: 40kHz

Peptide synthesizer, PS3 (Protein Technologies, Inc.)

- Synthesis Scale: 0.005 - 1.50 mMol (at 2x excess, up to 1.0 g of resin per RV)