LBRC Laboratories Guidelines BRP - Bioengineering Research Partnership Research's Guide

Main Laboratories and Facilities Equipment

2. TRI-MODAL SPECTROSCOPY AND MICROSCOPY LABORATORY
(6C-048)

Fluorescence and reflectance spectroscopy are used to detect diseases like dysplasia (pre-cancer) and atherosclerosis in vitro and in vivo in real time. A fiber optic based portable instrument with multiple excitation wavelengths was developed to study disease characteristic changes in fluorescence and reflectance spectra. A UV-visible fluorescence microscope is used to identify tissue fluorophores. An integrating sphere system is used to determine optical properties of tissue samples.

FastEEM System 4 Units
Lambda Physik Optex XeCl Excimer Laser Pumped Dye Lasers. Pulse energy 10 mJ (308 nm), dye lasers from 340 nm – 505 nm (~2 mJ); pulse width: 10 ns. Princeton Instruments I-Max 1024 Intensified CCD with an Acton Research ARC 150 Spectra Pro spectrograph. Hamamatsu flash lamp, Computer interfaced and real time data analysis

Fluorescence Microscope
Leitz Quartz Microscope, epi-fluorescence and transmission capabilities for UV-visible light. Fluorescence excitation light source: Coherent Innova 300 Argon Ion Laser UV Power 15 Watts (lines 333.6-363.8).

Integrating Sphere System
LabSphere Integrating Sphere with 8 inch diameter and Spectralon coated interior. Andor DU420-BU CCD camera with Acton Spectra Pro 300i spectrograph. 400W Oriel light source with UV-Vis wavelength range.