Enzyme-Mediated Assimilation of DNA-Functionalized Single-Walled Carbon Nanotubes

Research areas:
  • Uncategorized
Year: 2010
Type of Publication: Article
  • Clint M. Arnett, Charles R. Welch Charles P. Marsh
Journal: Langmuir Volume: 26
Number: 2
Month: JAN 19 2010
PT: J; TC: 0; UT: WOS:000273403400001
When pyrimidine-functionalized carbon nanotubes were incubated with single-stranded DNA ligase, formations of macroscopic aggregates were observed. Wet-cell transmission electron microscopy imaging revealed that the nanotubes were radially bound to form a 3D latticelike structure. These structures were not observed in control reactions lacking ligase or adenosine triphosphate. Raman spectroscopy analysis revealed no spectra indicative of carbon nanotubes in ligase-unamended controls; however, spectra were observed in radial breathing mode and in the G and G' bands in reactions containing ligase. Furthermore, the addition of deoxyribonuclease to the ligated reactions dispersed the aggregates, and a reduction in Raman spectral intensity was observed.