Hydroxyacids, energy storage, levulinic acid, hydroxyvalerate, biosynthesis, Ecoli, 4-hydroxyvalerate, 3-hydroxyvalerate, chiral synthesis
Biodegradable polyester (PHA) polymerization for energy storage, biosynthesis of antibiotics, pharmaceuticals and other chemicals
Yield efficiency on the production of long chain hydroxyacids monomers
This invention is an economical method for producing 4-hydroxyvalerate (4HV) and 3-hydroxyvalerate (3HV) from the inexpensive and renewable carbon source levulinic acid. These hydroxyvalerates were produced by periodically feeding levulinate to Psuedomonas putida KT2440 expressing the thioesterase II (tesB) gene from Escherichia coli K12. Once a suitable strain and enzyme system was found, the production process was optimized at the shake flask in minimal and rich media for the high-titer production of both 4HV and 3HV.
PCT filed April 3, 2009
Last revised: January 19, 2012
>>List of MIT Energy IP<<