Carlos Turrado Garcia

Capture of endogenous S-nitrosothiols for proteomics andimaging. Nitration of tyrosine residues in proteins is a low abundant post-translational modification. Protein nitration is associated with oxidative stress and increased nitric oxide (NO) synthase activity. It occurs both in physiological and in pathological conditions such as neurodegenerative processes (Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis), cancer and inflammatory diseases. This post-translational modification affects protein structure and function and even though it is known to be involved in the pathogenesis of some diseases and in normal regulatory processes, it has been difficult to study, mainly because the levels of 3-nitrotyrosine containing proteins are low compared to the levels of phosphorylated or nitrosylated proteins. Therefore, it is important to develop tools for the enrichment of modified proteins and detection with high selectivity and sensitivity. Here we describe two different approaches for the identification and visualization of 3-nitroproteins. The first is a new proteomics method for the determination of 3-nitrotyrosine containing proteins using new technologies in immunoprecipitation enrichment. In addition, based on a previously described method, a new class of probes for 3-nitrotyrosine containing peptides has been synthesized. These promising tools allow for both proteomics and imaging studies, and will provide a deeper understanding of the role of nitration in complex biological proteomes.

Carlos began his research in organic chemistry as an undergraduate in the photochemistry laboratory of Professor Diego Armesto Vilas at the Universidad Complutense de Madrid and later in the medicinal chemistry laboratory of Professor Mariá Luz López Rodríguez, leading to his BsC in 2005. Supported by an F.P.U. fellowship from the Spanish Ministroy of Education, he began his Ph.D. research in medicinal chemistry in 2006, supervised by professors López Rodríguez, Bellinda Benhamú Salama, and Silvia Ortega Gutiérrez, focusing on the design, synthesis and development of new synthetic inhibitors of fatty acid synthase enzymes. This work was additionally supported by a fellowship from the pharmaceutical company ITALFARMACO. He concurrently carried out a predoctoral project supervised by Professor Teresa Puig Miquel at the Hospital Universitari de Girona Doctor Josep Trueta and Universitat de Girona. He obtained his Ph.D. in organic chemistry from the Universidad Complutense de Madrid in 2013. Following a brief but successful period as a scientific junior in the medicinal chemistry department of the pharmaceutical company Almirall (2012), he joined the Wishnok/Tannenbaum mass spec lab in 2014 as a postdoctoral associate.
email Carlos.