contrasts.fit {limma}  R Documentation 
Given a linear model fit to microarray data, compute estimated coefficients and standard errors for a given set of contrasts.
contrasts.fit(fit, contrasts=NULL, coefficients=NULL)
fit 
an 
contrasts 
numeric matrix with rows corresponding to coefficients in 
coefficients 
vector indicating which coefficients are to be kept in the revised fit object. An alternative way to specify the 
This function accepts input from any of the functions lmFit
, lm.series
, mrlm
, gls.series
or lmscFit
.
The function reorientates the fitted model object from the coefficients of the original design matrix to any set of contrasts of the original coefficients.
The coefficients, unscaled standard deviations and correlation matrix are recalculated in terms of the contrasts.
The idea of this function is to fit a fullrank model using lmFit
or equivalent, then use contrasts.fit
to obtain coefficients and standard errors for any number of contrasts of the coefficients of the original model.
Unlike the design matrix input to lmFit
, which normally has one column for each treatment in the experiment, the matrix contrasts
may have any number of columns and these are not required to be linearly independent.
Methods of assessing differential expression, such as eBayes
or classifyTestsF
, can then be applied to fitted model object.
The coefficients
argument provides a simpler way to specify the contrasts
matrix when the desired contrasts are just a subset of the original coefficients.
Warning. For efficiency reasons, this function does not refactorize the design matrix for each probe. A consequence is that, if the design matrix is nonorthogonal and the original fit included quality weights or missing values, then the unscaled standard deviations produced by this function are approximate rather than exact. The approximation is usually acceptable. The results are always exact if the original fit was a oneway model.
An list object of the same class as fit
, usually MArrayLM
. This is a list with components
coefficients 
numeric matrix containing the estimated coefficients for each contrast for each probe. 
stdev.unscaled 
numeric matrix conformal with 

numeric 
... 
any other components found in 
Gordon Smyth
An overview of linear model functions in limma is given by 06.LinearModels.
# Simulate gene expression data: 6 microarrays and 100 genes # with one gene differentially expressed in first 3 arrays M < matrix(rnorm(100*6,sd=0.3),100,6) M[1,1:3] < M[1,1:3] + 2 # Design matrix corresponds to oneway layout, columns are orthogonal design < cbind(First3Arrays=c(1,1,1,0,0,0),Last3Arrays=c(0,0,0,1,1,1)) fit < lmFit(M,design=design) # Would like to consider original two estimates plus difference between first 3 and last 3 arrays contrast.matrix < cbind(First3=c(1,0),Last3=c(0,1),"Last3First3"=c(1,1)) fit2 < contrasts.fit(fit,contrast.matrix) fit2 < eBayes(fit2) # Large values of eb$t indicate differential expression results < classifyTestsF(fit2) vennCounts(results)