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Home > Molecule Pages > Glycan Binding Proteins > Galectins > Galectin 3 (human)
 
Galectin 3 (human)
in vitro 
Tissue Expression normal epithelial cells, fibroblasts, inflammatory and dendritic cells
Subcellular Localization cytoplasm, nucleus, exosomes, phagosomes

in vivo
KO Phenotype Maintenance of granulocyte numbers during acute peritonitis is defective in galectin-3-null mutant mice
  • reduced number of recoverable granulocytes after 4 days of thioglycollate induced peritonitis
  • granulocytes did not exhibit accelerated rates of apoptosis
  • macrophage phagocytosis of granulocytes was unaffected
Targeted disruption of the galectin-3 gene results in attenuated peritoneal inflammatory responses
  • fewer inflammatory cell infiltrations in the peritoneal cavities in response to thioglycollate broth treatment
  • lower levels of NF-kappaB response
  • different macrophage morphologies - spindle shaped
  • peritoneal macrophages more prone to undergo apoptosis when treated with apoptotic stimuli
Uncoupling of chondrocyte death and vascular invasion in mouse galectin 3 null mutant bones
  • abnormalities in the cells of the proliferative, mature, and hypertrophic zones and in the extracellular matrix of the hypertrophic zone
  • reduction in the total number of hypertrophic chondrocytes
  • subtle modification of Ihh expression in the galectin 3 mutant growth plate
  • striking difference was observed at the chondrovascular junction where many empty lacunae are present
  • large numbers of condensed chondrocytes exhibiting characteristic signs of cell death were found in the late hypertrophic zone
Accelerated diabetic glomerulopathy in galectin-3/AGE receptor 3 knockout mice
  • developed accelerated glomerulopathy in streptozotocin induced diabetes
  • increased proteinuria, extracellular matrix gene expression, and mesangial expansion
  • more marked renal/glomerular AGE accumulation
  • reduced expression of receptors implicated in AGE removal (macrophage scavenger receptor A and AGE-R1)
  • increased expression of receptors mediating cell activation (RAGE and AGE-R2)
Association of a macrophage galactoside-binding protein with Mycobacterium-containing phagosomes
  • reduced capacity to clear late but not early infection
Galectins-3 and -7, but not galectin-1, play a role in re-epithelialization of wounds
  • re-epithelialization of corneal wounds was significantly slower in two models of wound healing
  • corneal epithelial cell proliferation rate not perturbed
  • exogenous galectin-3 accelerated re-epithelialization in gal3(+/+) mice but, surprisingly, not in the gal3(-/-) mice
  • galectin-7 accelerated re-epithelialization of wounds in both gal3(+/+) and gal3(-/-) mice
  • healing corneas of gal3(-/-) mice contain markedly reduced levels of galectin-7
Critical role of galectin-3 in phagocytosis by macrophages
  • bone marrow derived macrophages exhibited reduced phagocytosis of IgG-opsonized erythrocytes and apoptotic thymocytes in vitro
  • attenuated phagocytic clearance of apoptotic thymocytes by peritoneal macrophages in vivo
  • reduced IgG-mediated phagocytosis of erythrocytes by Kupffer cells in a murine model of autoimmune hemolytic anemia
  • macrophages exhibited a lower degree of actin rearrangement upon Fcgamma receptor crosslinkage
Consortium KO Mice Phenotyping Data

Immunology

Histology

Hematology

Metabolism and Behavior

For general information on phenotyping, visit Core G.

Consortium KO Mice Glycoprofiling Data

Note: The MALDI-MS spectra are not completely annotated and thus are subject to change before entry into the Consortium database.
Mouse Strain Tissue
Kidney Spleen Thymus

Galectin-3
N-linked
(Low M.Wt)
(High M.Wt)
N-linked N-linked

Glycoprofiling Data of tissues from Wild type and other KO Mice



Diseases
Human Pathophysiology Tumor cell marker
  1. Anaplastic large cell lymphoma
  2. HTLV-I infected T cells
  3. Hepatocellular carcinoma
  4. Thyroid carcinoma
Prognostic indicator in tumors
  1. Decreased expression in breast cancer associated with increased progression
  2. Decreased expression in colon carcinoma and loss of nuclear localization associated with increased progression
  3. Decreased expression and loss of nuclear localization in uterine adenocarcinoma associated with increased invasiveness

 
 
 

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